Abstract
A culture of the bacterivorous rotifer H. rosa was separated from other Eucaryota in activated sludge and then purified by passages from most representatives of the bacterial microflora. A fully axenic culture was finally obtained by use of a lysing buffer and certain antibiotics.
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Gilleland, H. E. Jr, Stinnett, J. D., Eagon, R. 1974. Ultrastructural and chemical alteration of the cell envelope of Pseudomonas aeruginosa, associated with resistance to ethylenediamine-tetraacetate resulting from growth in Mg2+ deficient medium. J. Bact. 117: 302.
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Plasota, K., Plasota, M. & Kunicki-Goldfinger, W.J.H. Methods for obtaining an Axenic culture of Habrotrocha Rosa Donner 1949. Hydrobiologia 73, 37–38 (1980). https://doi.org/10.1007/BF00019420
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DOI: https://doi.org/10.1007/BF00019420