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The γ-subunit of spinach chloroplast ATP synthase: isolation and characterisation of cDNA and genomic clones

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Abstract

cDNA and genomic clones encoding the complete precursor polypeptide of the γ-subunit of spinach chloroplast ATP synthase have been isolated and characterised. The longest cDNA (1320 bp), selected from a λgt 11 spinach cDNA library, encoded a 364 amino acid residue protein (M r 40 028) that included a putative 41 residue N-terminal transit peptide. All the γ-subunit cDNAs analysed were derived from the same gene and Southern blot analysis ofBam HI restricted spinach DNA showed only one hybridising band (ca. 15 kb). Analysis of the cloned 15 kb genomic fragment, selected from a λEMBL4 spinach DNA library with a cDNA probe, confirmed there was a single gene for the γ-subunit. Sequencing, primer extension and northern blot analysis showed the gene contains two introns, 1066 and 665 bp in length, a 173 bp 5′ untranslated region and a 213 bp 3′ untranslated region. A 1450 nucleotide γ-subunit transcript was detected in RNA from light-grown and dark-grown spinach.

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Authors and Affiliations

  1. Division of Plant Industry, CSIRO, G.P.O. Box 1600, 2601, Canberra, ACT, Australia

    Paul R. Whitfeld

  2. Laboratoire de Biologie Cellulaire et Moleculaire, BIOSEM, 24 avenue des Landais, 63170, Aubière, France

    John G. Mason

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  1. John G. Mason
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Mason, J.G., Whitfeld, P.R. The γ-subunit of spinach chloroplast ATP synthase: isolation and characterisation of cDNA and genomic clones. Plant Mol Biol 14, 1007–1018 (1990). https://doi.org/10.1007/BF00019397

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  • DOI: https://doi.org/10.1007/BF00019397

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