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Thein vivo pattern of firefly luciferase expression in transgenic plants

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Abstract

Expression of the firefly luciferase gene in transgenic plants produces light emission patterns when the plants are supplied with luciferin. We explored whether inin vivo pattern of light emission truly reveals the pattern of luciferase gene expression or whether it reflects other parameters such as the availability of the substrate, luciferin, or the tissue-specific distribution of organelles in which luciferase was localized. The tissue-specific distribution of luciferase activity and thein vivo pattern of light were examined when the luciferase gene was driven by different promoters and when luciferase was redirected from the peroxisome, where it is normally targeted, to the chloroplast compartment. It was found that the distribution of luciferase activity closely correlated with the tissue-specific pattern of luciferase mRNA. However, thein vivo light pattern appeared to reflect not only tissue-specific distribution of luciferase activity, but also the pattern of luciferin uptake.

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Author notes

  1. Michel Schneider

    Present address: Sanofi Elf Bio Recherches, Labege-Innopole, BP 137, 31328, Labège Cedex, France

  2. David W. Ow

    Present address: USDA/University of California Berkeley Plant Gene Expression Center, 800 Buchanan Street, 94710, Albany, CA, USA

Authors and Affiliations

  1. Boyce Thompson Insitute, Cornell University, Tower Road, 14853-1801, Ithaca, NY, USA

    Stephen H. Howell

  2. Biology Department, University of California San Diego, 92093, LaJolla, CA, USA

    Michel Schneider & David W. Ow

Authors
  1. Michel Schneider
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  2. David W. Ow
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  3. Stephen H. Howell
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Schneider, M., Ow, D.W. & Howell, S.H. Thein vivo pattern of firefly luciferase expression in transgenic plants. Plant Mol Biol 14, 935–947 (1990). https://doi.org/10.1007/BF00019391

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  • DOI: https://doi.org/10.1007/BF00019391

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