Abstract
The kinetics of oxidation and reduction of P700, plastocyanin, cytochrome f and cytochrome b-563 were studied in a reconstituted system consisting of Photosystem I particles, cytochrome bf complex and plastocyanin, all derived from pea leaf chloroplasts. Decyl plastoquinol was the reductant of the bf complex. Turnovers of the system were initiated by laser flashes. The reaction between oxidised P700 and plastocyanin was non-homogeneous in that a second-order rate coefficient of c. 5×10−7 M−1 s−1 applied to 80% of the P700+ and c. 0.7×107 M−1 s−1 to the remainder. In the presence of bf complex, but without quinol, the electron transfer between cytochrome f and oxidised plastocyanin could be described by a second-order rate coefficient of c. 4×107 M−1 s−1 (forward), and c. 1.6×107 M−1 s−1 (reverse). The equilibrium coefficient was thus 2.5. Unexpectedly, there was little reduction of cytochrome f + or plastocyanin+ by electrons from the Rieske centre. With added quinol, reduction of cytochrome b-563 occurred. Concomitantly, electrons appeared in the oxidised species. It was inferred that either the Rieske centre was not involved in the high-potential chain of electron transfer events, or that, only in the presence of quinol, electrons were quickly passed from the Rieske centre to cytochrome f +. Additionally, the presence of quinol altered the equilibrium coefficient for the cyt f/PC interaction from 2.5 to c. 5. The reaction between quinol and the bf complex was describable by a second-order rate coefficient of about 3×106 M−1 s−1. The pattern of the redox reactions around the bf complex could be simulated in detail with a Q-cycle model as previously found for chloroplasts.
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Abbreviations
- AQS:
-
anthraquinone sulphonate
- cyt:
-
cytochrome
- cyt b-563(H):
-
high-potential cyt b-563
- cyt b-563(L):
-
low potential cyt b-563
- FeS(R):
-
the Rieske protein of the cyt bf complex, containing an Fe2S2 centre
- PC:
-
plastocyanin
- PS:
-
photosystem
- P700:
-
reaction centre in PS I
References
Chain RK and Malkin R (1991) The chloroplast cytochrome b 6 f complex can exist in monomeric and dimeric states. Photosynth Res 28: 59–68
Christensen HEM, Conrad LS and Ulstrup J (1992) Effects of NO2-modification of Tyr83 on the reactivity of spinach plastocyanin with cytochrome f. Biochim Biophys Acta 1099: 35–44
Gray JC (1978) Purification and properties of monomeric cytochrome f from Charlock, Sinapis arvensis L. Eur J Biochem 82: 133–141
Haehnel W, Propper A and Krause H (1980) Evidence of complexed plastocyanin as the immediate electron donor of P-700. Biochim Biophys Acta 593: 384–99
Haehnel W, Ratajczak R and Robenek H (1989) Lateral distribution and diffusion of plastocyanin in chloroplast thylakoids. J Cell Biol 108: 1397–1405
Hauska G (1986) Preparations of electrogenic, proton-transporting cytochrome complexes of the b 6 f-type (chloroplasts and cyanobacteria) and bc 1-type (Rhodopseudomonas sphaeroides). Methods Enzymol 126: 271–279
He S, Modi S, Bendall DS and Gray JC (1991) The surface-exposed tyrosine residue Tyr83 of pea plastocyanin in involved in both binding and electron transfer reactions with cytochrome f. EMBO J 10: 4011–4016
Hope AB (1993) The chloroplast cytochrome bf complex: A critical focus on function. Biochim Biophys Acta 1143: 1–22
Hope AB, Liggins J and Matthews DB (1989) The kinetics of reactions in and near the cytochrome b/f complex of chloroplasts. II. Cytochrome b-563 reduction. Aust J Plant Physiol 16: 353–364
Hope AB, Huilgol RR, Panizza M, Thompson M and Matthews DB (1992) The flash-induced turnover of cytochrome b-563, cytochrome f and plastocyanin in chloroplasts. Models and estimation of kinetic parameters. Biochim Biophys Acta 1100: 15–26
Jones RW and Whitmarsh J (1985) Origin of the electrogenic reaction in the chloroplast cytochrome b/f complex. Photobiochem Photobiophys 9: 119–27
Jones RW and Whitmarsh J (1988) Inhibition of electron transfer and the electrogenic reaction in the cytochrome b/f complex by 2-n-nonyl-4-hydroxyquinoline N-oxide (NQNO) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). Biochim Biophys Acta 933: 258–68
Malkin R (1992) Cytochrome bc 1 and b 6 f complexes of photosynthetic membranes. Photosynth Res 33: 121–136
Morand LZ, Frame M, Colvert KK, Johnson DA, Krogmann DW and Davis DJ (1989) Plastocyanin cytochrome f interaction. Biochemistry 28: 8039–8047
Morand LZ, Frame M, Krogmann DW and Davis DJ (1990) Characterisation of a covalently linked plastocyanin-cytochrome f adduct. In: Baltscheffky M (ed) Current Research in Photosynthesis, pp 303–305, Vol III. Kluwer Academic Publishers, Dordrecht
Moss DA and Rich PR (1987) The effect of pre-reduction of cytochrome b-563 on the electron-transfer reactions of the cytochrome bf complex in higher plant chloroplasts. Biochim. Biophys. Acta 894: 189–197
Mullet JE, Burke JJ and Arntzen CJ (1980) Chlorophyll proteins of Photosystem I. Plant Physiol 65: 814–822
Nitschke W, Hauska G and Crofts AR (1988) Fast electron transfer from low-to high-potential cytochrome b 6 in isolated cytochrome b 6 f complex. FEBS Lett 232: 204–208
Niwa S, Ishikawa H, Nikai S and Takabe T (1980) Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. J Biochem 88: 1177–1183
Qin L and Kostic NM (1992) Electron-transfer reactions of cytochrome f with flavin semiquinones and with plastocyanin. Importance of protein-protein electrostatic interactions and of donor-acceptor coupling. Biochemistry 31: 5145–5150
Ratajczak R, Mitchell R and Haehnel W (1988) Properties of the oxidising site of Photosystem I. Biochim Biophys Acta 933: 306–318
Rich PR, Heathcote P and Moss DA (1987) Kinetic studies of electron transfer in a hybrid system constructed from the cytochrome bf complex and Photosystem I. Biochim Biophys Acta 892: 138–51
Rich PR (1986) A perspective on Q-cycles. J Bioenerg Biomembr 18: 145–156
Selak MA and Whitmarsh J (1982) Kinetics of the electrogenic step and cytochrome b 6 and f redox changes in chloroplasts. FEBS Lett 150: 286–292.
Tanaka K, Takahashi M and Asada K (1981) Oxidation rate of monomeric radish cytochrome f with plastocyanin. Plant Cell Physiol 22: 33–39
Whitmarsh J, Bowyer JR and Crofts AR (1982) Modification of the apparent redox reaction between cytochrome f and the Rieske iron-sulfur protein. Biochim Biophys Acta 682: 404–412
Widger WR and Cramer WA (1991) The cytochrome b 6 /f complex. In: Bogorad L and Vasil IK (eds) Cell Culture and Somatic Cell Genetics of Plants, Vol 7B, The Molecular Biology of Plastids, ch4. Academic Press, San Diego
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Hope, A.B., Matthews, D.B. & Valente, P. The kinetics of reactions around the cytochrome bf complex studied in an isolated system. Photosynth Res 40, 199–206 (1994). https://doi.org/10.1007/BF00019337
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DOI: https://doi.org/10.1007/BF00019337