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Inflorescence-specific expression of AtK-1, a novel Arabidopsis thaliana homologue of shaggy/glycogen synthase kinase-3

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Abstract

We report here the isolation of the Arabidopsis thaliana gene AtK-1. The predicted protein sequence of AtK-1 show 70% identity to the Arabidopsis ASK and alfalfa MsK kinases that are homologs of the Drosophila shaggy and rat GSK-3 serine/threonine protein kinases playing an important role in signal transduction processes in animals. Northern analysis of different organs revealed exclusive expression in inflorescences suggesting an involvement of the AtK-1 kinase in reproduction-specific processes.

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References

  1. Bianchi MW, Guivarc'h D, Thomas M, Woodgett J, Kreis M: Arabidopsis homologs of the shaggy and GSK-3 protein kinases: molecular cloning and functional expression in Escherichia coli. Mol Gen Genet 242: 337–345 (1994).

    Google Scholar 

  2. Bourois M, Moore P, Ruel L, Grau Y, Heitzler P, Simpson P: An early embryonic product of the gene shaggy encodes a serine/threonine protein kinase related to the CDC28/cdc2+ subfamily. EMBO J 9: 2877–2884 (1990).

    Google Scholar 

  3. Boyle WB, Smeal T, Defize LHK, Angel P, Woodgett JR, Karin M, Hunter T: Activation of protein kinase C decreases phosphorylation of c-jun at sites that negatively regulate its DNA-binding activity. Cell 64: 573–584 (1991).

    Google Scholar 

  4. Brown JWS: A catalogue of splice junction and putative branch point sequences from plant introns. Nucl Acids Res 14: 9549–9559 (1986).

    Google Scholar 

  5. Chomzynski P, Sacchi N: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 165–159 (1987).

    Google Scholar 

  6. de Groot R, Auwerx J, Bourois M, Sassone-Corsi P: Negative regulation of jun/AP-1: conserved function of glycogen synthase kinase-3 and the Drosophila kinase shaggy. Oncogene 8: 841–847.

  7. Drews GN, Weigel D, Meyerowitz EM: Floral patterning. Curr Opin Gen Develop 1: 174–178 (1991).

    Google Scholar 

  8. Embi N, Rylatt DB, Cohen P: Glycogen synthase kinase-3 from rabbit skeletal muscle. Eur J Biochem 107: 519–527 (1980).

    Google Scholar 

  9. Hanks SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42–52 (1988).

    Google Scholar 

  10. Heitzler P, Simpson P: The choice of cell fate in epidermis of Drosophila. Cell 64: 1081–1092 (1991).

    Google Scholar 

  11. Hemmings BA, Yellowless D, Kernohan JC, Cohen P: Purification of glycogen synthase kinase 3 from rabbit skeletal muscle. Eur J Biochem 119: 443–451 (1982).

    Google Scholar 

  12. Hughes K, Nikolakaki A, Plyte SE, Totty NF, Woodgett JR: Modulation of the glycogen synthase kinase-3 family by tyrosine phosphorylation. EMBO J 12: 803–808 (1993).

    Google Scholar 

  13. Joshi CP: An inspection of the domain between putative TATA box and transcription start site in plant genes. Nucl Acids Res 15: 6643–6653 (1987).

    Google Scholar 

  14. Nikolakaki E, Coffer PJ, Hemelsoet R, Woodgett JR, Defize LHK: Glycogen synthase kinase-3 phosphorylates Jun family members in vitro and negatively regulates their transactivating potential in intact cells. Oncogene 8: 883–840 (1993).

    Google Scholar 

  15. Norman C, Runswick M, Pollock R, Treisman R: Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. Cell 55: 989–1003 (1988).

    Google Scholar 

  16. Passmore S, Maine GT, Elble R, Christ C, Tye BK: A Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MATa cells. J Mol Biol 2004: 593–606 (1988).

    Google Scholar 

  17. Pay A, Jonak C, Bögre L, Meskiene I, Mairinger T, Szalay A, Heberle-Bors E, Hirt H: The Msk family of alfalfa protein kinase genes encodes homologues of shaggy/glycogen synthase kinase-3 and shows differential expression patterns in plant organs and development. Plant J. 3: 847–856 (1993).

    Google Scholar 

  18. Ruel L, Pantesco V, Lutz Y, Simpson P and Bourouis M: Functional significance of a family of protein kinases encoded at the shaggy locus in Drosophila. EMBO J 12: 1657–1669 (1993).

    Google Scholar 

  19. Saksela K, Mäkelä TP, Hughes K, Woodgett JR, Alitalo K: Activation of protein kinase C increases phosphorylation of the L-myc transactivator domain at a GSK-3 target site. Oncogene 7: 347–353 (1992).

    Google Scholar 

  20. Siegfried E, Perkin LA, Capaci TM, Perrimon N: Putative protein kinase product of Drosophila segment polarity gene zeste-white 3. Nature 345: 825–828 (1990).

    Google Scholar 

  21. Siegfried E, Chou T-B Perrimon N: wingless signalling acts through zeste-white 3, the Drosophila homolog of glycogen synthase kinase-3, to regulate engrailed and establish cell fate. Cell 71: 1167–1179 (1992).

    Google Scholar 

  22. Vandeheede JR, Yand S-D, Goris J, Merleved W: ATP-Mg-dependent protein phosphate from rabbit skeletal muscle. J Biol Chem 255: 11768–11774 (1980).

    Google Scholar 

  23. Woodgett JR: Molecular cloning and expression of glycogen synthase kinase-3/factor A. EMBO J 9: 2431–2438 (1990).

    Google Scholar 

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Jonak, C., Heberle-Bors, E. & Hirt, H. Inflorescence-specific expression of AtK-1, a novel Arabidopsis thaliana homologue of shaggy/glycogen synthase kinase-3. Plant Mol Biol 27, 217–221 (1995). https://doi.org/10.1007/BF00019194

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  • DOI: https://doi.org/10.1007/BF00019194

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