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A proline-rich chitinase from Beta vulgaris

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Abstract

A gene (Chl) encoding a novel type of chitinase was isolated from Beta vulgaris. The Ch1 protein consists of an N-terminal hydrophobic prepeptide of 25 amino acids followed by a hevein-like domain of 22 amino acid residues, an unusually long proline-rich domain of 131 amino acid residues with 90 prolines, and finally a catalytic domain of 261 amino acid residues. Proteins with similar proline-rich domains are present in some other plants. The Chl gene shows a transient expression in response to fungal infection.

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References

  1. Benhamou N, Joosten MHAJ, de Wit PJGM: Subcellular localization of chitinase and of its potential substrate in tomato root tissues infected by Fusarium oxysporium f.sp. radicis-lycopersici. Plant Physiol 92: 1108–1120 (1990).

    Google Scholar 

  2. Bol JF, Linthorst HJ, Cornelissen BJ: Plant pathogenesis-related proteins induced by virus infection. Annu Rev Phytopath 28: 113–138 (1990).

    Google Scholar 

  3. Boller T, Gehri A, Mauch F, Vögeli U: Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. Planta 157: 22–31 (1983).

    Google Scholar 

  4. Bowles DJ: Defense-related proteins in higher plants. Annu Rev Biochem 59: 873–907 (1990).

    Google Scholar 

  5. Broglie KE, Gaynor JJ, Broglie RM: Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Natl Acad Sci USA 83: 6820–6824 (1986).

    Google Scholar 

  6. Broglie K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CF, Broglie R: Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254: 1194–1197 (1991).

    Google Scholar 

  7. Collinge DB, Slusarenko AJ: Plant gene expression in response to pathogens. Plant Mol Biol 9: 389–410 (1987).

    Google Scholar 

  8. Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K: Plant chitinases. Plant J 3: 31–40 (1993).

    Google Scholar 

  9. Dixon RA, Harrison M: Activation, structure and organization of genes involved in microbial defence in plants. Adv Genet 28: 165–234 (1990).

    Google Scholar 

  10. Gaynor JJ: Primary structure of an endochitinase mRNA from Solanum tuberosum. Nucl Acids Res 16: 5210 (1988).

    Google Scholar 

  11. Hart PJ, Monzingo AF, Ready MP, Ernst SR, Robertus JD: Crystal structure of an endochitinase from Hordeum vulgare L. seeds. J Mol Biol 229: 189–193 (1993).

    Google Scholar 

  12. Leah R, Tommerup H, Svendsen I, Mundy J: Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem 266: 1564–1573 (1991).

    Google Scholar 

  13. Linthorst HJM: Pathogenesis-related proteins of plants. Crit Rev Plant Sci 10: 123–150 (1991).

    Google Scholar 

  14. Mauch F, Mauch-Mani B, Boller T: Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiol 88: 936–942 (1988).

    Google Scholar 

  15. Metraux JP, Boller T: Local and systemic induction of chitinase in cucumber plants in response to viral, bacterial and fungal infections. Physiol Mol Plant Path 28: 161–169 (1986).

    Google Scholar 

  16. Mikkelsen JD, Berglund L, Nielsen KK, Christiansen H, Bojsen K: Structure of endochitinase genes from sugar beets. In: Brine C, Sandford SA, Zikakis JP (eds) Advances in Chitin and Chitosan, pp. 344–353. Elsevier Applied Science, Amsterdam (1992).

    Google Scholar 

  17. Neuhaus J-M, Sticher L, Meins F, Boller T: A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc Natl Acad Sci USA 88: 10362–10366 (1991).

    Google Scholar 

  18. Nielsen KK, Bojsen K, Collinge DB, Mikkelsen JD: Induced resistance in sugar beet against Cercospora beticola: induction by dichloroisonicotinic acid is independent of chitinase and β-1,3-glucanase transcript accumulation. Physiol Mol Plant Path, in press (1994).

  19. Nielsen KK, Bojsen K, Roepstorff P, Mikkelsen JD: A hydroxyproline-containing class IV chitinase of sugar beet is glycosylated with xylose. Plant Mol Biol, in press (1994).

  20. Nielsen KK, Jørgensen P, Mikkelsen JD: Antifungal activity of sugar beet chitinase against Cercospora beticola: an autoradiographic study on cell wall degradation. Plant Path, in press (1994).

  21. Nielsen KK, Mikkelsen JD, Kragh KM, Bojsen K: An acidic class III chitinase in sugar beet: induction by Cercospora beticola, characterization, and expression in transgenic tobacco plants. Mol Plant-Microbe Interact 6: 495–506 (1993).

    Google Scholar 

  22. Parsons TJ, Bradshaw HD, Gordon MP: Systemic accumulation of specific mRNAs in response to wounding in poplar trees. Proc Natl Acad Sci USA 87: 7895–7899 (1989).

    Google Scholar 

  23. Payne G, Ahl P, Moyer M, Harper A, Beck J, Meins F, Ryals J: Isolation of complementary DNA clones encoding pathogenesis-related proteins P and Q, two acidic chitinases from tobacco. Proc Natl Acad Sci USA 87: 98–102 (1990).

    Google Scholar 

  24. Raikel NV, Lee H-I, Broekaert WF: Structure and function of chitin-binding proteins. Annu Rev Plant Physiol Plant Mol Biol 44: 591–615 (1993).

    Google Scholar 

  25. Schlumbaum A, Mauch F, Vogeli U, Boller T: Plant chitinases are potent inhibitors of fungal growth. Nature 324: 365–367 (1986).

    Google Scholar 

  26. Shinshi H, Mohnen D, Meins F: Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci USA 84: 89–93 (1987).

    Google Scholar 

  27. Sticher L, Hofsteenge J, Milani A, Neuhaus JM, Meins F: Vacuolar chitinases of tobacco: A new class of hydroxyproline-containing proteins. Science 257: 655–657 (1992).

    Google Scholar 

  28. Tata SJ, Beitema JJ, Balabaskaran S: The lysozyme of Hevea brasiliensis lates. Isolation, purification, enzyme kinetics and a partial amino acid sequence. J Rubber Res Inst Malaysia 31: 35–48 (1983).

    Google Scholar 

  29. Verburg JG, Smith CE, Lisek CA, Khai Huynh Q: Identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from Zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminoproply)-carbodiimide. J Biol Chem 267: 3886–3893 (1992).

    Google Scholar 

  30. von Heijne G: Patterns of Amino acids near signal-sequence cleavage sites. Eur J Biochem 133: 17–21 (1983).

    Google Scholar 

  31. Wessels JGH, Sietsma JH: Fungal cell walls: a survey. In: Tanner W, Loewus FA (eds) Encyclopedia of Plant Physiology, New Series, Vol 13B, Plant Carbohydrates II, pp. 352–394. Springer-Verlag, Berlin (1981).

    Google Scholar 

  32. Wubben JP, Joosten MHAJ, Van Kan JAL, de Wit PJGM: Subcellular localization of plant chitinases and 1–3,-b-glucanases in Cladosporium fulvum (syn. Fulvia fulva)-infected tomato leaves. Physiol Mol Plant Path 41: 23–32 (1992).

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000, Aarhus C, Denmark

    Lars Berglund, Zhaochun Chen & Kjeld A. Marcker

  2. Danisco Biotechnology, Langebrogade 1, DK-1001, Copenhagen, Denmark

    Lars Berglund, Janne Brunstedt, Klaus K. Nielsen & Jørn D. Mikkelsen

Authors
  1. Lars Berglund
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  2. Janne Brunstedt
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  3. Klaus K. Nielsen
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  4. Zhaochun Chen
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  5. Jørn D. Mikkelsen
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  6. Kjeld A. Marcker
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Berglund, L., Brunstedt, J., Nielsen, K.K. et al. A proline-rich chitinase from Beta vulgaris . Plant Mol Biol 27, 211–216 (1995). https://doi.org/10.1007/BF00019193

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  • DOI: https://doi.org/10.1007/BF00019193

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