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Hole burning study of excited state structure and energy transfer dynamics of bacteriochlorophyll c in chlorosomes of green sulphur photosynthetic bacteria

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Abstract

Results of low temperature fluorescence and spectral hole burning experiments with whole cells and isolated chlorosomes of the green sulfur bacterium Chlorobium limicola containing BChl c are reported. At least two spectral forms of BChl c (short-wavelength and long-wavelength absorbing BChl c) were identified in the second derivative fluorescence spectra. The widths of persistent holes burned in the fluorescence spectrum of BChl c are determined by excited state lifetimes due to fast energy transfer. Different excited state lifetimes for both BChl c forms were observed. A site distribution function of the lowest excited state of chlorosomal BChl c was revealed. The excited state lifetimes are strongly influenced by redox conditions of the solution. At anaerobic conditions the lifetime of 5.3 ps corresponds to the rate of energy transfer between BChl c clusters. This time shortens to 2.6 ps at aerobic conditions. The shortening may be caused by introducing a quencher. Spectral bands observed in the fluorescence of isolated chlorosomes were attributed to monomeric and lower state aggregates of BChl c. These forms are not functionally connected with the chlorosome.

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Abbreviations

BChl:

bacteriochlorophyll

EET:

electronic energy transfer

FWHM:

full width at half maximum

SDF:

site distribution function

RC:

reaction centre

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Authors and Affiliations

  1. Department of Chemical Physics, Faculty of Mathematics & Physics, Charles University, Ke Karlovu 3, 121 16, Prague2, Czech Republic

    Jakub P\>sen\>cík, Martin Vácha, Franti\>sek Adamec, Milan Ambro\>z & Juraj Dian

  2. Institute of Microbiology CAV, Opatovický mlýn, 379 81, Trebon, Czech Republic

    Jan Bo\>cek

Authors
  1. Jakub P\>sen\>cík
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  2. Martin Vácha
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  3. Franti\>sek Adamec
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  4. Milan Ambro\>z
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  5. Juraj Dian
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  6. Jan Bo\>cek
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  7. Jan Hála
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P\>sen\>cík, J., Vácha, M., Adamec, F. et al. Hole burning study of excited state structure and energy transfer dynamics of bacteriochlorophyll c in chlorosomes of green sulphur photosynthetic bacteria. Photosynth Res 42, 1–8 (1994). https://doi.org/10.1007/BF00019052

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  • DOI: https://doi.org/10.1007/BF00019052

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