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Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana

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Abstract

Genes homologous to the auxin-inducible Nt103 glutathione S-transferase (GST) gene of tobacco, were isolated from a genomic library of Arabidopsis thaliana. We isolated a λ clone containing an auxin-inducible gene, At103-1a, and part of a constitutively expressed gene, At103-1b. The coding regions of the Arabidopsis genes were highly homologous to each other and to the coding region of the tobacco gene but distinct from the GST genes that have been isolated from arabidopsis thusfar. Overexpression of a cDNA clone in Escherichia coli revealed that the AT103-1A protein had GST activity.

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, RUL-TNO Centre for Phytotechnogy, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands

    Dianne A. M. van der Kop, Monique Schuyer, Bert J. van der Zaal & Paul J. J. Hooykaas

  2. Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Ben Scheres

Authors
  1. Dianne A. M. van der Kop
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  2. Monique Schuyer
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  3. Ben Scheres
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  4. Bert J. van der Zaal
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  5. Paul J. J. Hooykaas
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van der Kop, D.A.M., Schuyer, M., Scheres, B. et al. Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana . Plant Mol Biol 30, 839–844 (1996). https://doi.org/10.1007/BF00019016

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  • DOI: https://doi.org/10.1007/BF00019016

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