Abstract
We have isolated a gene from bean (Vicia faba L.), called Vein1, that encodes a novel protein. The Vein1 cDNA was isolated as a result of a differential screen for genes that are expressed in leaves but not in the most common cell type, the mesophyll cell. Northern blot analysis revealed that Vein1 transcripts are differentially expressed in the plant with expression in leaves, stems and sepals but not in petals, mesophyll cells or roots. In situ hybridization studies of stem and leaf sections indicate that the expression of Vein1 is localized to the phloem tissue. Interestingly, Vein1 was differentially expressed in stem tissue with the highest expression in the oldest internodes. The deduced Vein1 protein sequence does not share homology with any known protein sequences. The 17 kDa Vein 1 protein is highly hydrophilic and contains a histidine-rich motif, where six out of seven amino acids are histidines. The function of Vein1 is unknown, although the expression patterns suggests that it may play a role in mature phloem tissue in the aerial parts of the plant.
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References
Bostwick DE, Dannenhoffer JM, Skaggs MI, Lister RM, Larkins BA, Thompson GA: Pumpkin phloem lectin genes are specifically expressed in companion cells. Plant Cell 4: 1539–1528 (1992).
Cassab GI, Varner JE: Cell wall proteins. Annu Rev Plant Physiol 39: 321–353 (1988).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19–21 (1983).
DeWitt ND, Harper JF, Sussman MR: Evidence for a plasma membrane proton pump in phloem cells of higher plants. Plant J 1: 121–128 (1991).
Domingo SC, Gomez MD, Canas L, Herandez-Yago J, Conejero V, Vera P: A novel extracellular matix protein from tomato associated with lignified secondary cell walls. Plant Cell 6: 1035–1047 (1994).
Edwards JW, Walker EL, Coruzzi GM: Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proc Natl Acad Sci USA 87: 3459–3463 (1990).
Foley RC, Grossman C, Ellis JG, Llewellyn DJ, Dennis ES, Peacock WJ, Singh KB: Isolation of a maize bZIP subfamily; candidates for the ocs-element transcription factor. Plant J 3: 669–679 (1993).
Foley RC, Singh KB: Isolation of Vicia faba metallothionein-like gene: expression in foliar trichomes. Plant Mol Biol 2: 435–444 (1994).
Herouart D, van Montagu M, Inze D: Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco. Plant Physiol 104: 873–880 (1994).
Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943 (1989).
Ishiwatari Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H, Chino M: Thioredoxin h is one of the major proteins in rice phloem sap. Planta 195: 456–463 (1995).
Kieliszewski MJ, Kamyab A, Leykam JF, Lamport DTA: A histidine-rich extensin from Zea mays is an arabinogalactan protein. Plant Physiol 99: 538–547 (1992).
McKhann HI, Hirsch AM: In situ localization of specific mRNAs in plant tissues. In: Glick BR, Thompson JE (eds) Methods in Plant Molecular Biology and Biotechnology, pp. 179–205. CRC Press, Boca Raton, FL (1993).
Nolte KD, Koch KE: Companion-cell specific localization of sucrose synthase in zones of phloem loading and unloading. Plant Physiol 101: 899–905 (1993).
Read SM, Northcote DH: Subunit structure and interactions of the phloem proteins of Cucurbita maxima (pumpkin). Eur J Biochem 134: 561–569 (1983).
Ryser U, Keller B: Ultrastructural localization of a bean glycine-rich protein in unlignified primary walls of protoxylem cells. Plant Cell 4: 773–783 (1992).
Sheng J, D'Ovidio R, Mehdy MC: Negative and positive regulation of a novel proline-rich protein mRNA by fungal elicitor and wounding. Plant J 1: 345–354 (1991).
Showalter AM: Structure and function of plant cell wall proteins. Plant Cell 5: 9–23 (1993).
Smith LM, Sabnis DD, Johnson RPC: Immunochemical localization of phloem lectin from Cucurbita maxima using peroxidase and colloidal-gold labels. Planta 170: 461–470 (1987).
Stiefel V, Ruiz-Avila L, Raz R, Valles MP, Gomez J, Pages M, Martinez-Izquierdo JA, Ludevid MD, Langdale JA, Nelson T, Puigdomenech P: Expression of a maize cell wall hydroxyproline-rich glycoprotein gene in early leaf and root vascular differentiation. Plant Cell 2: 785–793 (1990).
Truernit E, Sauer N: The promoter of the Arabidopsis thaliana SUC2 sucrose-H + symporter gene directs expression of β-glucuronidase to the phloem: Evidence for phloem loading and unloading by SUC2. Planta 196: 564–570 (1995).
Walker TS: The purification and some properties of a protein causing gelling in phloem sieve tube exudate from Cucurbita pepo. Biochim Biophys Acta 257: 433–444 (1992).
Wyatt RE, Nagao RT, Key JL: Patterns of soybean proline-rich protein gene expression. Plant Cell 4: 99–110 (1992).
Yang NS, Russell D: Maize sucrose synthase-1 promoter directs phloem cell-specific expression of GUS gene in transgenic plants. Proc Natl Acad Sci USA 87: 4144–4148 (1990).
Ye Z-H, Song Y-R, Marcus A, Varner JE: Comparative localization of three classes of cell wall proteins. Plant J 1: 175–183 (1991).
Ye Z-H, Varner JE: Tissue-specific expression of cell wall proteins in developing soybean tissues. Plant Cell 3: 23–37 (1991).
Zhang S, Medhy MC: Binding of a 50 kD protein to a u-rich sequence in an mRNA encoding a proline-rich protein that is destabilized by fungal elicitor. Plant Cell 6: 135–145 (1994).
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Foley, R.C., Liang, Z.M. & Singh, K.B. A novel phloem-specific gene is expressed preferentially in aerial portions of Vicia faba . Plant Mol Biol 30, 687–695 (1996). https://doi.org/10.1007/BF00019004
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DOI: https://doi.org/10.1007/BF00019004