Abstract
Two rice cDNA clones (COS6 and COS9) were isolated, corresponding to genes that were highly expressed in roots from seedlings and mature plants. A genomic clone (GOS9) corresponding to cDNA clone COS9 was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. 5′ ends and 3′ ends of the mRNA were determined by primer extension and S1-nuclease mapping respectively. The open reading frame present in GOS9 potentially encodes a protein (14kDa) that does not show any significant homology to other proteins in databases.
This is a preview of subscription content, log in via an institution to check access.
References
Conkling MA, Cheng C-L, Yamamoto YT, Goodman HM: Isolation of transcriptionally regulated root-specific genes from tobacco. Plant Physiol 93: 1203–1211 (1990).
Keller B, Lamb CJ: Specific expression of a novel cell wall hydroxyproline glycoprotein gene in lateral root initiation. Genes Devel 3: 1639–1646 (1989).
Lerner DR, Raikhel NV: Cloning and characterization of root-specific barley lectin. Plant Physiol 91: 124–129 (1989).
dePater S, Hensgens LAM, Schilperoort RA: Structure and expression of a light-inducible shoot-specific rice gene. Plant Mol Biol 15: 399–406 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Pater, B.S., Schilperoort, R.A. Structure and expression of a root-specific rice gene. Plant Mol Biol 18, 161–164 (1992). https://doi.org/10.1007/BF00018473
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00018473