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PCR cloning of the full-length cDNA for the seed protein canavalin from the jack bean plant,Canavalis ensiformis

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References

  1. Mullis KB, Faloona FA, Scharf SJ, Saiki RK, Horn GT, Erlich HA: Specific enzymatic amplification of DNAin vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 51: 263–273 (1986).

    PubMed  Google Scholar 

  2. Krug MS, Berger SL: First-stranded cDNA synthesis primed with oligo(dT). Meth Enzymol 152: 316–325 (1987).

    PubMed  Google Scholar 

  3. Yamauchi D, Nakamura K, Asahi T, Ninamikawa T: cDNAs for canavalin and concanavalin A fromCanavalia gladiata. Eur J Biochem 170: 515–520 (1988).

    PubMed  Google Scholar 

  4. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  5. Gibbs FEM, Strongin KB, McPherson A: Evolution of legume seed storage protein: a domain common to legumins and vicilins is duplicated in vicilins. Mol Biol Evol 6: 614–623 (1989).

    PubMed  Google Scholar 

  6. Ko TP, Ng JD, Day J, Greenwood A, Greene M, McPherson A: Cloning, expression and crystallographic analysis of the reserve proteins of the jack bean. In preparation. (1991).

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Ng, J.D., Stinchcombe, T., Ko, TP. et al. PCR cloning of the full-length cDNA for the seed protein canavalin from the jack bean plant,Canavalis ensiformis . Plant Mol Biol 18, 147–149 (1992). https://doi.org/10.1007/BF00018469

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