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Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii

Photosynthesis Research volume 44, pages 191–205 (1995)Cite this article

Abstract

Chloroplast transformation of Chlamydomonas reinhardtii has developed into a powerful tool for studying the structure, function and assembly of thylakoid protein complexes in a eukaryotic organism. In this article we review the progress that is being made in the development of procedures for efficient chloroplast transformation. This focuses on the development of selectable markers and the use of Chlamydomonas mutants, individually lacking thylakoid protein complexes, as recipients. Chloroplast transformation has now been used to engineer all four major thylakoid protein complexes, photosystem II, photosystem I, cytochrome b 6/f and ATP synthase. These results are discussed with an emphasis on new insights into assembly and function of these complexes in chloroplasts as compared with their prokaryotic counterparts.

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Abbreviations

ENDOR:

electron nuclear double resonance

ESEEM:

electron spin echo envelope modulation

LHC:

light harvesting complex

PSI:

Photosystem I

PS II:

Photosystem II

P680 :

primary electron donor in PS II

P700 :

primary electron donor in PS I

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  1. Department of Botany and Center for the Study of Early Events in Photosynthesis, Arizona State University, Box 871601, 85287-1601, Tempe, AZ, USA

    Andrew N. Webber, Scott E. Bingham & Hyeonmoo Lee

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  1. Andrew N. Webber
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  2. Scott E. Bingham
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  3. Hyeonmoo Lee
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Webber, A.N., Bingham, S.E. & Lee, H. Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii . Photosynth Res 44, 191–205 (1995). https://doi.org/10.1007/BF00018309

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Key words

  • photosynthesis
  • specific mutagenesis
  • chloroplast DNA