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Phototransformation of protochlorophyllideF657 in etiochloroplasts isolated from pine cotyledons; dark reformation of this pigment-complex from a pool of ALA-protochlorophyllideF635 in the presence of NADPH

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Abstract

Etiochloroplasts isolated from dark-grown pine cotyledons fed with δ-aminolevulinic acid (ALAFootnote 1) contain, in addition to the chlorophyll forms, two protochlorophyllide complexes which emit fluorescence around 635 nm and 657 nm respectively (ALA-PChlideF635 and PChlideF657). By a combination of light flashes and periods of darkness, it is possible to phototransform PChlideF657 and thereafter, if NADPH is added to the system, to re-form this pigment-complex from the pool of ALA-PChlideF635 during the dark periods. The process of phototransformation followed by the re-formation of PChlideF657 in the presence of NADPH can be obtained in vitro five to six times consecutively. The role of NADPH in the formation of the PChlideF657 complex is discussed.

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Notes

  1. Abbreviations: ALA=\Gd-aminolevulinic acid; Chl(ide) = chlorophyll(ide); PChlide = protochlorophyllide; PChlideA636, PChlideA650=forms of protochlorophyllide absorbing light maximally at the wavelength indicated in nm; PChlideF635, PChlideF657=PChlide forms exhibiting a fluorescence maximum at the wavelength indicated; ALA-Pchlide=PChlide formed by an exogenous ALA treatment; NADPH = nicotineamide adeninedinucleotide phosphate.

References

  1. Brouers M and Sironval C (1974) Evidence for energy transfer from protochlorophyllide to chlorophyllide in leaves treated with δ-aminolevulinic acid. Plant Sci Lett 2: 67–72.

    Google Scholar 

  2. Brouers M and Wolwertz MR (1980) Stability of etiochloroplasts isolated from pine cotyledons as studied by low temperature absorption and fluorescence spectroscopy. Photosynthesis Res. 1: 93–104.

    Google Scholar 

  3. Griffiths W (1974) Protochlorophyll and protochlorophyllide as precursors for chlorophyll synthesis in vitro. FEBS Lett 49: 196–200.

    Google Scholar 

  4. Griffiths W (1975) Some observations on chlorophyll(ide) synthesis by isolated etioplasts. Biochem J 146: 17–24.

    Google Scholar 

  5. Griffiths W (1975) Characterization of the terminal stages of chlorophyll(ide) synthesis in etioplast membrane preparations. Biochem J 152: 623–635.

    Google Scholar 

  6. Griffiths W (1974) Source of reducing equivalents for the in vitro synthesis of chlorophyll from protochlorophyll. FEBS Lett 46: 301–304.

    Google Scholar 

  7. Mattheis J and Rebeiz C (1977) Chloroplast biogenesis. Net synthesis of protochlorophyllide from protoporphyrin IX by developing chloroplasts. J B Chem 252: 8347–8349.

    Google Scholar 

  8. Michel-Wolwertz MR and Bronchart R (1974) Formation of prolamellar bodies without correlative accumulation of protochlorophyllide or chlorophyllide in pine cotyledons. Plant Sci Lett 2: 45–54.

    Google Scholar 

  9. Michel-Wolwertz MR (1977) Chlorophyll formation in cotyledons of Pinus jeffreyi during germination in the dark, occasional accumulation of protochlorophyll(ide) forms. Plant Sci Lett 8: 125–134.

    Google Scholar 

  10. 10.Oku T, Sugahara K and Tomita G (1974) Functional development of photosystems I and II in dark-grown pine seedlings. Plant Cell Physiol 15: 175–178.

    Google Scholar 

  11. Rebeiz CA and Castelfranco PA (1971) Chlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol 47: 33–37.

    Google Scholar 

  12. sundqvist Chr (1969) Transformation of PChlide, formed from exogenous δ-aminolevulinic acid, in continuous light or flashlight. Physiol Plant 22: 147–156.

    Google Scholar 

  13. Sundqvist Chr (1970) The conversion of protochlorophyllide636 to protochlorophyllide650 in leaves treated with δ-aminolevulinic acid. Physiol Plant 23: 412–424.

    Google Scholar 

  14. VonWettstein D (1958) The formation of plastid structures. The photochemical apparatus, its structure and function. Brookl Symp Biol 11: 138–159. New York: Upton.

    Google Scholar 

  15. Wolwertz M-R (1978) Two alternative pathways of chlorophyll biosynthesis in Pinus jeffreyi. In Akoyunoglou G et al., eds. Chloroplast development, pp. 111–118.

  16. Brodersen P (1976) Factors affecting the photoconversion of protochlorophyllide to chlorophyllide in etioplast membranes isolated from barley. Photosynthetica 10: 33–39.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biochemical Systematics, Department of Botany, University of Liège, Sart Tilman B.22, B-4000, Liège, Belgium

    M. R. Wolwertz & M. Brouers

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  1. M. R. Wolwertz
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  2. M. Brouers
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Wolwertz, M.R., Brouers, M. Phototransformation of protochlorophyllideF657 in etiochloroplasts isolated from pine cotyledons; dark reformation of this pigment-complex from a pool of ALA-protochlorophyllideF635 in the presence of NADPH. Photosynth Res 1, 105–113 (1980). https://doi.org/10.1007/BF00018227

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  • DOI: https://doi.org/10.1007/BF00018227

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