Abstract
A portable field system for simultaneous measurement of transpiration and CO2 exchange from leaves of fruit trees is described. CO2 concentration is measured by means of infra-red gas analysis, using small gas samples collected in syringes. Methods for analysing small gas samples are compared. The leaf chamber described can also be used in a conventional laboratory open gas-exchange system, its small volume permitting measurement of very rapid changes in gas exchange in response to experimental stimuli.
Resumen
Este artículo describe un sistema portátil para medir simultaneamente transpiración e intercambio de CO2 en hojas de árboles frutales en el campo. Para medir la velocidad de fotosíntesis se colectan pequeñas muestras de gas en jeringas y se inyectan al analizador de gases de luz infra-roja. Se comparan varios métodos para analizar las muestras. Se describe la cámara de absorción de CO2 utilizada, la cual puede tambien incorporarse a un sistema abierto convencional, su pequeño volumen permite detectar cambios rápidos en el intercambio de gases cuando se producen alteraciones en el ambiente.
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References
Austin RB and Longden PC (1967) A rapid method for the measurement of rates of photosynthesis using 14CO2. Ann Bot 31: 245–253.
Bosian G (1964) Der peltiereffekt im einsatz sur kuvettenklimaregulierung. Ber Dtsch Bot Ges 77: 22–23.
Bravdo BA (1972) Photosynthesis, transpiration, leaf stomatal and mesophyll resistance measurements by the use of a ventilated diffusion porometer. Physiol Plant 27: 209–215.
Cary JW (1977) Relation between CO2 exchange rate, CO2 compensation, and mesophyll resistance from a simple field method. Crop Sci 17: 454–457.
Clegg MD and Sullivan CY (1975) A rapid method for measuring carbon dioxide concentrations. Agron Abstr p. 70.
Clegg MD, Sullivan CY and Eastin JD (1978) A sensitive technique for rapid measurement of carbon dioxide concentrations. Plant Physiol 62: 924–926.
Day W (1977) A direct reading continuous flow porometer. Agric Meteor 18: 81–89.
Fernández J (1978) A simple system to determine photosynthesis in field conditions by means of 14CO2. Photosynthetica 12: 145–149.
Jarvis PG (1971) The estimation of resistances to carbon dioxide transfer. In: Sëstak Z, Cätzky J and Jarvis PG, eds. Plant photosynthetic production. Manual of methods, pp. 566–622. The Hague: Junk.
Jones HG (1973) Limiting factors in photosynthesis. New Phytol 72: 1089–1094.
Jones HG and Norton TA (1979) Internal factors controlling the rate of evaporation from fronds of some intertidal algae. New Phytol 83: 771–781.
Kriedemann PE and Smart RE (1971) Effects of irradiance, temperature, and leaf water potential on photosynthesis of vine leaves. Photosynthetica 5: 6–15.
Lange OL (1962) Eine ‘klapp-kuvette’ zur CO2-gaswechselregistrierung and blattern von freil and pflanzen mit dem URAS. Ber. Dtsch Bot Ges 75: 41–50.
Lange OL and Schulze ED (1971) Measurement of CO2 gas-exchange and transpiration in the beech (Fagus silvatica L.). In: Ellenberg H, ed. Ecological studies. Analysis and synthesis, vol. 2, pp 16–28. Berlin: Springer-Verlag.
Larson DW and Kershaw KA (1975) Measurement of CO2 exchange in lichens: a new method. Can J Bot 53: 1535–1541.
Monteith JL (1973) Principles of environmental physics, pp 135–136. London: Edward Arnold.
Parkinson KJ and Day W (1979) The use of orifices to control the flow rate of gases. J Appl Ecol 16: 623–632.
Parkinson KJ, Day W, Croft J, Day AT, French BK, Leach JE, Dunlop MW, Joyce J, Scott P and Strange SA (1978) Rothamsted Exp Sta Report, Part 1.
Parkinson KJ and Legg BJ (1972) A continuous flow porometer. J Appl Ecol 9: 669–675.
Parkinson KJ and Legg BJ (1978) Calibration of infra-red analysers for carbon dioxide. Photosynthetica 12: 65–67.
Sestak Z, Catsky J and Jarvis PG (1971) Plant photosynthetic production: manual of methods. The Hague: Junk.
Strebeyko P (1967) Rapid method for measuring photosynthetic rates using 14CO2. Photosynthetica 1: 45–49.
Tranquillini R (1957) Standortklima, wasserbilanz and CO2-gaswechsel junger Zirben (Pinus cembra L.) an der alpinen waldgrenze. Planta 49: 612–661.
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Fanjul, L., Jones, H.G. & Treharne, K.J. A portable system for simultaneous measurement of transpiration and CO2 exchange. Photosynth Res 1, 83–92 (1980). https://doi.org/10.1007/BF00018225
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DOI: https://doi.org/10.1007/BF00018225