Skip to main content

Rapid determination of free proline for water-stress studies


Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.

This is a preview of subscription content, access via your institution.


  1. 1

    Barnett, N. M. and Naylor, A. W., Plant Physiol. 41, 1222 (1966).

    Google Scholar 

  2. 2

    Chinard, E. P., J. Biol. Chem. 199, 91 (1952).

    PubMed  Google Scholar 

  3. 3

    Kimura, Y., Anal. Chim. Acta. 37, 240 (1967).

    Article  PubMed  Google Scholar 

  4. 4

    Messer, M., Anal. Biochem. 2, 353 (1961).

    PubMed  Google Scholar 

  5. 5

    Palfi, G. and Juhasz, J., Plant and Soil 34, 503 (1971).

    Google Scholar 

  6. 6

    Thompson, J. F., et al., Plant Physiol. 41, 1578 (1966).

    Google Scholar 

  7. 7

    Troll, W., and Lindsley, J., J. Biol. Chem. 215, 655 (1955).

    PubMed  Google Scholar 

  8. 8

    Wren, J. J. and Wiggall, P. H., Biochem. J. 94 216 (1965)

    PubMed  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bates, L.S., Waldren, R.P. & Teare, I.D. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207 (1973).

Download citation


  • Plant Physiology
  • Proline
  • Water Stress
  • Leaf Material
  • Rapid Determination