Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.
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Bates, L.S., Waldren, R.P. & Teare, I.D. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207 (1973). https://doi.org/10.1007/BF00018060
- Plant Physiology
- Water Stress
- Leaf Material
- Rapid Determination