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Rapid determination of free proline for water-stress studies

Summary

Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.

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Bates, L.S., Waldren, R.P. & Teare, I.D. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207 (1973). https://doi.org/10.1007/BF00018060

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Keywords

  • Plant Physiology
  • Proline
  • Water Stress
  • Leaf Material
  • Rapid Determination