Summary
Humic substances extracted with chelating resin from a Dark Brown Chernozem (Typic Haploboroll) Ah, a Black Chernozem (Udic Haploboroll) Ah, and a Dark Gray Luvisol (Typic Cryoboralf) Ahe were fractionated by Bio-Gel P. The eluted material was combined to form three or four fractions for further characterization.
Most of the extracted polydisperse humic substances of the three soils were eluted near V0 and distributed around a single mean value. The ash content of this material varied from 1.36 to 1.78 per cent. Carboxyl acidity may thus be obtained without interference from any cations.
The humic substances from the Dark Brown and Black Chernozem Ah gave similar elution profiles, whereas those from the Dark Gray Luvisol Ahe were less aliphatic and more aromatic in nature. The materials of low molecular weight had the highest content of ash, C, and carboxyl groups. They represented only 3.6 to 14.1 per cent of the C extracted and were considered, on the basis of increased C content, decreased optical density, and infrared spectral configurations, to consist of polynuclear, fused-ring, aromatic structures.
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Dormaar, J.F. Molecular-sieve chromatography of humic substances extracted with chelating resin from chernozemic Ah horizons. Plant Soil 41, 51–64 (1974). https://doi.org/10.1007/BF00017943
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DOI: https://doi.org/10.1007/BF00017943