Abstract
Cleavage of Vicia faba nuclear DNA with the restriction endonuclease BamHI yielded discrete size classes of 250, 850, 900, 990, 1 150, 1 500 and 1 750 bp of highly repetitive DNA. Each of these sequence families comprised about 3% of the total genomic DNA. Some sequence members from each sequence family were cloned in pBR322 and their primary structures determined. Computer analyses of nucleotide sequences suggested the existence of about 60 bp sequence periodicity within the repeating unit of the 990 bp sequence family, though the extent of homology among the surmised shorter subrepeat units was very low. With other BamHI sequence families, however, the data did not show any clear internal sequence periodicity. The repeat units of the 850 bp and 1 750 bp sequence families contained nucleotide sequences homologous to the 250 bp family sequence. No sequence relationship between or among other sequence families was observed. There was 13–25% sequence variation among 6 cloned members of the 250 bp family and probably also among those of other BamHI repeat families. DNA sequences homologous to these V. faba BamHI repeat families were detected in Pisum sativum DNA by Southern blot hybridization. Furthermore, very weak cross-hybridization was observed with plant DNAs from Phaseolus vulgaris, Triticum aestivum, Cucumis sativus and Trillium kamtschaticum.
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Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB: A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560, 1980.
Carlson M, Brutlag D: Cloning and characterization of a complex satellite DNA from Drosophila melanogaster. Cell 11:371–381, 1977.
Chambers CA, Schell MP, Skinner DM: The primary sequence of a Crustacean satellite DNA containing a family of repeats. Cell 13:97–110, 1978.
Deumling B: Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla, a tandemly repeated inverted repeat. Proc Natl Acad Sci USA 78:338–342, 1981.
Döring H-P, Starlinger P: Barbara McClintock's controlling elements: Now at the DNA level. Cell 39:253–259, 1984.
Endow SA: Analysis of Drosophila melanogaster satellite IV with restriction endonuclease MboII. J Mol Biol 114: 441–449, 1977.
Harr R, Hagblom P, Gustafson P: Two-dimensional graphic analysis of DNA sequence homologies. Nucl Acids Res 10:365–374, 1982.
Hemleven V, Lewek B, Roth A, Stadler J: Organization of highly repetitive satellite DNA of two Cucurbitaceae species (Cucumis melo and Cucumis sativus). Nucl Acids Res 10:631–644, 1982.
Holmes DS, Quigley M: A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114: 193–197, 1981.
Hörz W, Altenburger W: Nucleotide sequence of mouse satellite DNA. Nucl Acids Res 9:683–696, 1981.
Howley PM, Israel MA, Law Ming-Fan, Martin MA: A rapid method for detecting and mapping homology between heterogeneous DNAs. J Biol Chem 254:4876–4883, 1979.
Kato A, Yakura K, Tanifuji S: Sequence analysis of Vicia faba repeated DNA, the FokI repeat element. Nucl Acids Res 12:6415–6425, 1984.
Manuelidis L: Nucleotide sequence definition of major human repeated DNA, the HindIII 1.9 kb family. Nucl Acids Res 10:3211–3219, 1982.
Maxam AM, Gilbert W: Sequencing end-labeled DNA with base-specific chemical cleavages. In: Grossman L (ed) Methods in enzymology, Vol. 65, Academic Press, New York, 1980, pp 449–560.
Novotny J: Matrix program to analyze primary structure homology. Nucl Acids Res 10:127–131, 1982.
Peacock WJ, Dennis ES, Rhoades MM, Pryor AJ: Highly repeated DNA sequence limited to knob heterochromatin in maize. Proc Natl Acad Sci USA 78:4490–4494, 1981.
Plucienniczak A, Akowronski J, Jaworski J: Nucleotide sequence of bovine 1.715 satellite DNA and its relation to other bovine satellite sequences. J Mol Biol 158:293–304, 1982.
Rowland RE: Distribution of repetitive DNA sequences in Vicia faba chromosomes. Exp Cell Res 126:444–448, 1980.
Sellers H: On the theory and computation of evolution distances. SIAM J Appl Math 26:787–793, 1974.
Shepherd NS, Schwarz-Sommer Z, Blumberg vel Spalve J, Gupta M, Wienand U, Saedler H: Similarity of the Cinl repetitive family of Zea mays to eukaryotic transposable elements. Nature 307:185–187, 1984.
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517, 1975.
Taparowsky EJ, Gerbi SA: Sequence analysis of bovine satellite I DNA (1.715 gm/cm3). Nucl Acids Res 10: 1271–1281, 1982.
Timmis JN, Deumling B, Ingle J: Localisation of satellite DNA sequences in nuclei and chromosomes of two plants. Nature 257:152–155, 1975.
Yakura K, Fukuei K, Tanifuji S: Chromatin subunit structure in different tissues of higher plants. Plant & Cell Physiol 19:1381–1390, 1978.
Yakura K, Kato A, Tanifuji S: Length heterogeneity of the large spacer of Vicia faba rDNA is due to the differing number of a 325 bp repetitive sequence element. Mol Gen Genet 193:400–405, 1984.
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Kato, A., Iida, Y., Yakura, K. et al. Sequence analysis of Vicia faba highly repeated DNA: the BamHI repeated sequence families. Plant Mol Biol 5, 41–53 (1985). https://doi.org/10.1007/BF00017872
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DOI: https://doi.org/10.1007/BF00017872