Abstract
The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment(t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5′ A/TTTTATPuTTTA/T 3′) essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4).
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Ohtani, T., Kiyokawa, S., Ohgawara, T. et al. Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast. Plant Mol Biol 5, 35–39 (1985). https://doi.org/10.1007/BF00017871
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DOI: https://doi.org/10.1007/BF00017871