Abstract
A gene, sts14, coding for a highly expressed mRNA in pistils of Solanum tuberosum, was isolated. Northern blot and in situ analyses demonstrated that the gene was expressed throughout pistil development in both the stylar cortex and the stigma. The deduced STS14 protein displays similarity to the pathogenesis-related PR-1 proteins. A possible function for protection or guidance of the pollen tubes through the pistil is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atkinson AH, Heath RL, Simpson RJ, Clarke AE, Anderson MA: Proteinase inhibitors in Nicotiana alata stigmas are derived from a precursor protein which is processed into 5 homologous inhibitors. Plant Cell 5: 203–213 (1993).
Casacuberta JM, Puigdomènech P, Segundo BS: A gene coding for a basic pathogenesis-related (PR-like) protein from Zea mays. Molecular cloning and induction by a fungus (Fusarium moniliforme) in germinating maize seeds. Plant Mol Biol 16: 527–536 (1991).
Cheung AY: Pollen-pistil interactions in compatible pollination. Proc Natl Acad Sci USA 92: 3077–3080 (1995).
Constabel CP, Brisson N: Stigma- and vascular-specific expression of the PR-10a gene of potato: A novel pattern of expression of a pathogenesis-related gene. Mol Plant-Microbe Interact 8: 104–113 (1995).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Gu Q, Kawata EE, Morse M-J, Wu H-M, Cheung AY: A flower-specific cDNA encoding a novel thionin in tobacco. Mol Gen Genet 234: 89–96 (1992).
Herrero M, Arbelo A: Influence of the pistil on pollen tube kinetics in peach (Prunus persica). Am J Bot 76: 1441–1447 (1989).
Hülskamp M, Schneitz K, Pruitt RE: Genetic evidence for a long-range activity that directs pollen tube guidance in Arabidopsis. Plant Cell 7: 57–64 (1995).
Joshi CP: Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucl Acids Res 15: 9627–9640 (1987).
Kandasamy MK, Nasrallah JB, Nasrallah ME: Pollenpistil interactions and developmental regulation of pollen tube growth in Arabidopsis. Development 120: 3405–3418 (1994).
Karunanandaa B, Singh A, Kao TH: Characterization of a predominantly pistil-expressed gene encoding a γ-thionin-like protein of Petunia inflata. Plant Mol Biol 26: 459–464 (1994).
Kroh M, Miki-Hirosige H, Rosen W, Loewus F: Incorporation of label into pollen tube walls from myoinositol-labeled Lilium longiflorum pistils. Plant Physiol 45: 92–94 (1970).
Labarca C, Loewus F: The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. Plant Physiol 52: 87–92 (1973).
Leung DWM: Involvement of plant chitinase in sexual reproduction of higher plants. Phytochemistry 31: 1899–1900 (1992).
Lord EM, Sanders LC: Roles for the extracellular matrix in plant development and pollination: a special case of cell movement in plants. Devel Biol 153: 16–28 (1992).
Lotan T, Ori N, Fluhr R: Pathogenesis-related proteins are developmentally regulated in tobacco flowers. Plant Cell 1: 881–887 (1989).
Mo Y, Nagel C, Taylor LP: Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc Natl Acad Sci USA 89: 7213–7217 (1992).
Muradov A, Petrasovits L, Davidson A, Scott KJ: A cDNA clone for a pathogenesis-related protein 1 from barley. Plant Mol Biol 23: 439–442 (1993).
Neale AD, Wahleithner JA, Lund M, Bonnett HT, Kelly A, Meeks-Wagner DR, Peacock WJ, Dennis ES: Chitinase, B-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. Plant Cell 2: 673–684 (1990).
Ori N, Sessa G, Lotan T, Himmelhoch S, Fluhr R: A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass. EMBO J 9: 3429–3436 (1990).
Pfitzner UM, Goodman HM: Isolation and characterization of cDNA clones encoding pathogenesis-related proteins from tobacco mosaic virus infected tobacco plants. Nucl Acids Res 15: 4449–4465 (1987).
Szybiak-Strozycka U, Lescure N, Cullimore JV, Gamas P: A cDNA encoding a PR-1-like protein in the model legume Medicago truncatula. Plant Physiol 107: 273–274 (1995).
Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E, Ryals J: Acquired resistance in Arabidopsis. Plant Cell 4: 645–656 (1992).
van Eldik GJ, Vriezen HW, Wingens M, Ruiter RK, van Herpen MMA, Schrauwen JAM, Wullems GJ: A pistil-specific gene of Solanum tuberosum is predominantly expressed in the stylar cortex. Sex Plant Reprod 8: 173–179 (1995).
van Kan JAL, Joosten MHAJ, Wagemakers CAM, van den Berg-Velthuis GCM, de Wit PJGM: Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato induced by virulent and avirulent races of Cladosporium fulvum. Plant Mol Biol 20: 513–527 (1992).
Von Heijne G: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683–4690 (1986).
Ylstra B, Busscher J, Franken J, Hollman PCH, Mol JNM, van Tunen AJ: Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth. Plant J 6: 201–212 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Eldik, G.J., Wingens, M., Ruiter, R.K. et al. Molecular analysis of a pistil-specific gene expressed in the stigma and stylar cortex of Solanum tuberosum . Plant Mol Biol 30, 171–176 (1996). https://doi.org/10.1007/BF00017811
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00017811