Abstract
The marine bryozoan Amathia wilsoni contains several brominated secondary metabolites, the alkaloid amathamides A–F. Energy dispersive X-ray analysis coupled with scanning electron microscopy was used to localise bromine in sections of Amathia wilsoni. Bromine concentrations higher than background levels were only found on the surface of the bryozoan and not within any of the different internal cell types. The correlation between bromine levels and a rod-shaped bacterium, ubiquitous to the tip region, points to the bacterium being closely associated with the range of amathamides found in Amathia wilsoni.
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Anthoni, U., P. H. Neilson, M. Pereira & C. Christophersen, 1990. Bryozoan secondary metabolites: a chemotaxonomical challenge. Comp. Biochem. Physiol. 96B: 431–437.
Araragi, M., 1979. Comparison of actinomycete flora between tropical and Japanese upland farm soils. 1. Physiological characteristics. Soil Sci. Plant Nutr. 25: 245–254.
Becker, R. P. & M. Sogard, 1979. Visualization of subsurface structures in cells and tissues by backscattered electron imaging. Scanning Electron Microscopy 3: 835–870.
Blackman, A. J. & S. L. Fu, 1991. A ß —phenylethylamine derived possible biosynthetic precursor to the amathamides, alkaloids from the Bryozoan Amathia wilsoni. J. Nat. Prod. 52: 436.
Blackman, A. J. & R. D. Green, 1987. Further amathamide alkaloids from the bryozoan Amathia wilsoni. Aust. J. Chem. 40: 1655–1562.
Blackman, A. J. & D. J. Mathews, 1985. Amathamide alkaloids from the marine bryozoan Amathia wilsoni Kirkpatrick. Heterocycles 23: 2829–2833.
Blankenship, J. E., 1976. Tetrodotoxin: from poison to powerful tool. Persp. Biol. Med. 19: 509–512.
Burkholder, P. R., R. M. Pfister & F. H. Leitz, 1966. Production of a pyrrole antibiotic by a marine bacterium. Appl. Microbiol. 14: 649–653.
Jandik, P. & W. R. Jones, 1991. Optimization of detection sensitivity in the capillary electrophoresis of inorganic anions. J. Chromatogr. 546: 431–433.
Mocros, A. S., 1973. A table for the ionic composition of seawater based on 1967 atomic weights. J. Cons. Int. explor. Mer. 35: 94–95.
Noguchi, T., J. Jeon, O. Arakawa, H. Sugita, Y. Deguchi, Y. Shida & K. Hashimoto, 1986. Occurrence of tetrodotoxin in Vibrio. sp. isolated from the intestines of a xanthid crab, Atergatis floridus. J. Biochem. 99: 331–335.
Noguchi, T., H. Narita, J. Maruyama & K. Hashimoto, 1982. Tetrodotoxin in the starfish Astropecten polyacanthus in association with toxification of a trumpet shell, ‘Boshibora’ Charonia sauliae. Bull. Japan. Soc. scient. Fish. 48: 1173–1177.
Okami, Y., 1986. Marine microorganism as a source of bioactive agents. Microb. Ecol. 12: 65–78.
Pouchou, J.-L. & F. Pichoir, 1984. A new model for quantitative x-ray microanalysis, part I: application to the analegens of homogenous samples. La Recherche Aerospatiale 3: 167–192.
Thompson, J. E., K. D. Barrow & D. J. Faulkner, 1983. Localization of Two Brominated Metabolites, Aerothionin and Homoaerothionin, in Spherulous Cells of the Marine Sponge Aplysina fistularis (= Verongia thiona). Acta Zool. 64: 199–210.
Thuesen, E. V., K. Kogure, K. Hashimoto & T. Nemoto, 1988. Poison arrowworms: a tetrodotoxin venom in the marine phylum Chaetognatha. J. exp. mar. Biol. Ecol. 116: 249–256.
Walls, J. T., A. J. Blackman & D. A. Ritz, 1991. Distribution of amathamide alkaloids within single colonies of the bryozoan Amathia wilsoni. J. Chem. Ecol. 17: 1871–1881.
Walls, J. T., D. A. Ritz & A. J. Blackman, 1983. Fouling, surface bacteria and antibacterial agents of four bryozoan species found in Tasmania, Australia J. exp. mar. Biol. Ecol. 169: 1–13.
Yotsu, M., T. Yamazaki, Y. Meguro, A. Endo, M. Murata, H. Naoki & T. Yasumoto, 1987. Production of tetrodotoxin and its derivatives by Pseudomonas sp. Isolated from the skin of a pufferfish. Toxicon 25: 225–228.
Young, D. N., B. M. Howard & W. Fenical, 1980. Subcellular localization of brominated secondary metabolites in the red alga Laurencia synderae. J. Phycol. 16: 182–185.
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Walls, J.T., Blackman, A.J. & Ritz, D.A. Localisation of the amathamide alkaloids in surface bacteria of Amathia wilsoni Kirkpatrick, 1888 (Bryozoa: Ctenostomata). Hydrobiologia 297, 163–172 (1995). https://doi.org/10.1007/BF00017482
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DOI: https://doi.org/10.1007/BF00017482