Abstract
Two-dimensional gel electrophoresis of in vitro translation products was used to examine differences between the steady state RNA populations of an untransformed tobacco plant line and a non-rooting tobacco shoot line transformed with a T l -DNA segment from Agrobacterium tumefaciens carrying the cytokinin gene (T-cyt). The analysis comprised about 240 translation products representing the more abundant mRNAs. Approximately 8% of the translation products were found to have significantly different concentrations, due to both increases and decreases, when the shoot parts of the transformed and untransformed lines were compared. Only a few of these differences were specific for the comparison of transformed and untransformed shoots. Most of the differences were also observed when the shoot and root parts of the untransformed line were compared. This implies that the shoot or root prevalence of several mRNA species in normal plants is altered in transgenic T-cyt shoots. The observed changes in the mRNA population of transgenic T-cyt shoots are discussed in relation to the transformed phenotype and previously cloned mRNAs showing similar changes in tissue-specific prevalence.
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References
Burrell MM, Temple S, Ooms G: Changes in translatable poly(A) RNA from differentiated potato tissues transformed with shoot-inducing Ti TL-DNA from Agrobacterium tumefacies. Plant Mol Biol 6: 213–220 (1986).
Chen C-M, Leisner SM: Cytokinin-modulated gene expression in excised pumpkin cotyledons. Plant Physiol 77: 99–103 (1985).
De Vries SC, Springer J, Wessels JGH: Diversity of abundant mRNA sequences and patterns of protein synthesis in etiolated and greened pea seedlings. Planta 156: 129–135 (1982).
Eichholz R, Harper J, Felix G, Meins FJr: Evidence for an abundant 33,000-dalton polypeptide regulated by cytokinins in cultured tobacco tissues. Planta 158: 410–415 (1983).
Felix G, Meins F.Jr: Purification, immunoassay and characterization of an abundant, cytokinin-regulated polypeptide in cultured tobacco tissues. Planta 164: 423–428 (1985).
Flores S, Tobin EM: Benzyladenine modulation of the expression of two genes for nuclear-encoded chloroplast proteins in Lemna gibba: Apparent posttranscriptional regulation. Planta 168: 340–349 (1986).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Linsmaier EM, Skoog F: Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 8: 100–127 (1965).
Memelink J, de Pater BS, Hoge JHC, Schilperoort RA: T-DNA hormone biosynthetic genes: Phytohormones and gene expression in plants. Dev Genet 8: 321–337 (1987).
Memelink J, Hoge JHC, Schilperoort RA: Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco. EMBO J 6: 3579–3583 (1987).
Meyer Y, Aspart L, Chartier Y: Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplasts cultivated in vitro. I. Characteristics of auxin-sensitive proteins. Plant Physiol 75: 1027–1033 (1984).
Meyer Y, Aspart L, Chartier Y: Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplasts cultivated in vitro. II. Time course and level of auxin control. Plant Physiol 75: 1034–1039 (1984).
Mohnen D, Shinshi H, Felix G, Meins FJr: Hormonal regulation of β-1,3-glucanase messenger RNA levels in cultured tobacco tissues. EMBO J 4: 1631–1635 (1985).
O'Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007–4021 (1975).
Peerbolte R, Floor M, Ruigrok P, Hoge JHC, Wullems GJ, Schilperoort RA: Stability and expression of transferred DNA in F1 tobacco transformants studied at various states of differentiation. Planta 172: 448–462 (1987).
Shinshi H, Mohnen D, Meins FJr: Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci USA 84: 89–93 (1987).
Teyssendier de la Serve B, Axelos M, Péaud-Lenoël C: Cytokinins modulate the expression of genes encoding the protein of the light-harvesting chlorophyll a/b complex. Plant Mol Biol 5: 155–163 (1985).
Theologis A: Rapid gene regulation by auxin. Ann Rev Plant Physiol 37: 407–438 (1986).
Van der Zaal EJ, Memelink J, Mennes AM, Quint A, Libbenga KR: Auxin-induced mRNA species in tobacco cell cultures. Plant Mol Biol 10: 145–157 (1987).
Van der Zaal EJ, Mennes AM, Libbenga KR: Auxin-induced rapid changes in translatable mRNAs in tobacco cell suspensions. Planta 172: 514–519 (1987).
Van Slogteren GMS, Hoge JHC, Hooykaas PJJ, Schilperoort RA: Clonal analysis of heterogeneous crown gall tumor tissues induced by wild-type and shooter mutant strains of Agrobacterium tumefaciens-expression of T-DNA genes. Plant Mol Biol 2: 321–333 (1983).
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Memelink, J., de Vries, S.C., Schilperoort, R.A. et al. Changes in the tissue-specific prevalence of translatable mRNAs in transgenic tobacco shoots containing the T-DNA cytokinin gene. Plant Mol Biol 11, 625–631 (1988). https://doi.org/10.1007/BF00017462
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DOI: https://doi.org/10.1007/BF00017462