Abstract
The objective of this investigation was to quantify the differences in soil carbon stores after exposure of birch seedlings (Betula pendula Roth.) over one growing season to ambient and elevated carbon dioxide concentrations. One-year-old seedling of birch were transplanted to pots containing ‘C4 soil’ derived from beneath a maize crop, and placed in ambient (350 μL L−1) and elevated (600 μL L−1) plots in a free-air carbon dioxide enrichment (FACE) experiment. After 186 days the plants and soils were destructively sampled, and analysed for differences in root and stem biomass, total plant tissue and soil C contents and δ13C values. The trees showed a significant increase (+50%) in root biomass, but stem and leaf biomasses were not significantly affected by treatment. C isotope analyses of leaves and fine roots showed that the isotopic signal from the ambient and elevated CO2 supply was sufficiently distinct from that of the ‘C4 soil’ to enable quantification of net root C input to the soil under both ambient and elevated CO2. After 186 days, the pots under ambient conditions contained 3.5 g of C as intact root material, and had gained an additional 0.6 g C added to the soil through root exudation/turnover; comparable figures for the pots under elevated CO2 were 5.9 g C and 1.5 g C, respectively. These data confirm the importance of soils as an enhanced sink for C under elevated atmospheric CO2 concentrations. We propose the use of ‘C4 soils’ in elevated CO2 experiments as an important technique for the quantification of root net C inputs under both ambient and elevated CO2 treatments.
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References
Amthor, J S 1995 Terrestrial higher-plant response to increasing atmospheric [CO2] in relation to the global carbon cycle. Global Change Biol. 1, 243–274.
Andreux, F, Cerri, C, Vose, P B and Vitorello, V A 1989 Potential of stable isotope, 15N and 13C, methods for determining input and turnover in soils. In Nutrient Cycling in Terrestrial Ecosystems. Eds. A FHarrison, PIneson and O WHeal. pp 259–275. Elsevier Applied Science, London.
Billès, G, Rouhier, H and Bottner, P 1993 Modifications of the carbon and nitrogen allocations in the plant (Triticum aestivum L.) soil system in response to increased atmospheric CO2 concentration. Plant and Soil 157, 215–225.
Cotrufo, M F and Ineson, P 1995 Effects of enhanced CO2 and nutrient supply on the quality and subsequent decomposition of fine roots of Betula pendula Roth. and Picea sitchensis (Bong.) Carr. Plant and Soil 170, 267–277.
Curtis, P S, O'Neill, E G, Teeri, J A, Zak, D R and Pregitzer, K S 1994 Belowground responses to rising atmospheric CO2: Implications for plants, soil biota and ecosystem processes. Plant and Soil 165, 1–6.
Diaz, S, Grime, J P, Harris, J and McPherson, E 1993 Evidence of a feedback mechanism limiting plant response to elevated carbon dioxide. Nature 364, 616–617.
Eamus, D and Jarvis, P G 1989 The direct effects of increases in the global atmospheric CO2 concentration on natural and commercial temperate trees and forests. Adv. Ecol. Res. 19, 1–55.
Hendrey, G R, Lewin, K F and Nagy, J 1993 Free air carbon dioxide enrichment: development, progress, results. Vegetatio 104/105, 17–31.
Houghton, R A 1993 Is carbon accumulating in the Northern temperate zone? Global Biogeochem. Cycles 7, 611–617.
Leavitt, S W, Paul, E A, Kimball, B A, Hendrey, G R, Mauney, J R, Rauschkolb, R S, Rogers, H, Lewin, K F, Nagy, J, Pinter, P J and Johnson, H B 1994 Carbon-isotope dynamics of free-air CO2-enriched cotton and soils. Agric. For. Met. 70, 87–101.
Lekkerkerk, L J A, Van deGeijn, S C and VanVeen, J A 1990 Effects of elevated atmospheric CO2 levels on the carbon economy of a soil planted with wheat. In Soils and the Greenhouse Effect. Ed. A FBouwman. pp 423–429. John Wiley and Sons, New York.
Mary, B, Mariotti, A and Morel, J 1992 Use of 13C variations at natural abundance for studying the biodegradation of root mucilage, roots and glucose in soil. Soil Biol. Biochem. 24, 1065–1072.
Norby, R J, O'Neill, E G, Hood, W G and Luxmoore, R J 1987 Carbon allocation, root exudation and mycorrhizal colonization of Pinus echinata seedlings grown under CO2 enrichment. Tree Physiol. 3, 203–210.
Norby, R J 1994 Issues and perspectives for investigating root responses to elevated atmospheric carbon dioxide. Plant and Soil 165, 9–20.
Rogers, H G, Runion, G B and Krupa, S V 1994 Plant responses to atmospheric CO2 enrichment with emphasis on roots and the rhizosphere. Environ. Pollut. 83, 155–189.
Rouhier, H, Billès, G, El Kohen, A, Mousseau, M and Bottner, P 1994 Effects of elevated CO2 on carbon and nitrogen distribution within a tree (Castanea sativa Mill.)-soil system. Plant and Soil. 162, 281–292.
Smith, B N and Epstein, S 1971 Two categories of 13C/12C ratios for higher plants. Plant Physiol. 47, 380–384.
Swinnen, J 1994 Evaluation of the use of a model rhizodeposition technique to separate root and microbial respiration in soil. Plant and Soil 165, 89–101.
Van deGeijn, S C and vanVeen, J A 1993 Implications of increased carbon dioxide levels for carbon input and turnover in soils. Vegetatio 104/105, 283–292.
VanVeen, J A, Liljeroth, E, Lekkerkerk, L J A and van deGeijn, S C 1991 Carbon fluxes in plant-soil systems at elevated atmospheric CO2 levels. Ecol. Appl. 1, 175–181.
Whipps, J M 1985 Effects of CO2 concentration on growth carbon distribution and loss of carbon from the roots of maize. J. Exp. Bot. 36, 645–651.
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Ineson, P., Cotrufo, M.F., Bol, R. et al. Quantification of soil carbon inputs under elevated CO2: C3 plants in a C4 soil. Plant Soil 187, 345–350 (1995). https://doi.org/10.1007/BF00017099
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DOI: https://doi.org/10.1007/BF00017099