Abstract
When N deficient 10-day-old wheat plants were supplied with 1.5 mol m−3 NH4 +, net NH4 + uptake rapidly decreased during the first 6h, while root free-NH4 + and free amino acids concentration increased. However, after 24 h the NH4 + uptake rate increased again, as the internal NH4 + concentration decreased. When plants were pretreated during 40 h with different external NH4 + concentrations, net uptake measured on 1.0 mol m−3 NH4 + decreased with the increasing ion concentration during the pretreatment. This decrement coincided with both root free-NH4 + and total free amino acids levels. When N-starved and NH4 + fed plants were treated during 0, 3 or 6 h with 1.0 mol m−3 NH4 + in the presence of 1.0 mol m−3 MSX2, net uptake (measured without MSX) decreased with the length of the inhibitor treatment. In both groups, MSX significantly increased root free-NH4 + concentration, while the level of total free amino acids was only increased in N-starved plants. When N-starved plants were externally supplied with 1.0 mol m−3 of different amino acids or amides, net NH4 + uptake was only strongly inhibited in the presence of glutamine or asparagine. It is concluded that rapid changes in the concentration of certain amino acids during NH4 + nutrition might regulate the ion absorption, though at high endogenous levels of free NH4 + net uptake could be suppressed independently of the root concentration of free amino acids.
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Abbreviations
- AUR:
-
ammonium uptake rate
- MSX:
-
L-methionine sulphoximine
References
Arima Y 1979 15N-nitrate assimilation in association with glutamine synthesis in rice seedling roots. Soil Sci. Pl. Nutr. 25, 311–322.
Arima Y and Kumazawa K 1977 Evidence of ammonium assimilation via the glutamine synthetase-glutamate synthase system in rice seedling roots. Plant Cell Physiol. 18, 1121–1129.
Arnozis P A and Barneix A J 1989 PEP-carboxylase activity during ammonium assimilation in wheat plants. J. Pl. Nutr. 12, 85–94.
Arnozis P A and Findenegg G R 1986 Electrical charge balance in the xylem sap of Beet and Sorghum plants grown with either NO3 − or NH4 + nitrogen. J. Plant Physiol. 125, 441–449.
Arnozis P A, Nelemans J A and Findenegg G R 1988 Posphoenolpyruvate carboxylase activity in plants grown with either NO3 − or NH4 + as inorganic nitrogen sources. J. Plant. Physiol. 132, 23–27.
Breteler H and Arnozis P A 1985 Effect of amino compounds on nitrate utilization by roots of dwarf bean. Phytochemistry 24, 653–657.
Cataldo D, Schrader L and Youngs V 1974 Analysis by digestion and colorimetric assay of total nitrogen in plant tissues high in nitrate. Crop Sci. 14, 854–856.
Clarkson D T 1986 Regulation of the absorption and release of nitrate by plant cells: A review of current ideas and methodology. In Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Eds. H Lambers, J J Neeteson and I Stulen. Vol. 19 pp 3–27. Martinus Nijhoff Publishers, Dordrecht.
Clarkson D T, Saker L R and Purves J V 1989 Depression of nitrate and ammonium transport in barley plants with diminished sulfate status: Evidence of co-regulation of nitrogen and sulfate intake. J. Exp. Bot. 218, 953–963.
Cooper H D and Clarkson D T 1989 Cycling of amino nitrogen and other nutrients between shoots and roots in cereals: A possible mechanism integrating shoot and root in the regulation of nutrient uptake. J. Exp. Bot. 216, 1–10.
Doddema H and Otten H 1979 Uptake of nitrate by mutants of Arabidopsis thaliana disturbed in uptake or reduction of nitrate. III. Regulation. Physiol. Plant. 45, 339–346.
Etherthon B and Rubinstein B 1977 Evidence for amino acid-H+ cotransport in oat coleoptiles. Plant Physiol. 59, 117 (Abs).
Goyal S and Huffaker R C 1986 The uptake of NO3 −, NO2 − and NH4 + by intact wheat (Triticum aestivum) seedlings. Plant Physiol. 82, 1051–1056.
Jackson E A, Kwik K D and Volk R J 1976 Nitrate influx and efflux by intact wheat seedlings: Effects of prior nitrate nutrition. Planta 132, 149–156.
Joy K W 1988 Ammonia, glutamine and asparagine: A carbon — nitrogen interface. Can. J. Bot. 66, 2103–2109.
Kropf D L, Caldwell J H, Gow N A R and Harold F M 1984 Transcellular ion currents in the water mold Achlya: Amino acid proton symport as a mechanism of current entry. J. Cell Biol. 99, 486–496.
Lee R B and Rudges K A 1986 Effect of nitrogen deficiency on the absorption of nitrate and ammonium by barley plants. Ann. Bot. 57, 471–486.
MacKown C T 1987 Nitrate uptake following nitrate deprivation. J. Exp. Bot. 38, 1079–1090.
MacKown C T, Volk R J and Jackson W A 1981 Nitrate accumulation, assimilation and transport by decapitated corn roots: Effects of prior nitrate nutrition. Plant Physiol. 68, 135–138.
Mengel K and Viro M 1978 The significance of plant energy status for the uptake and incorporation of NH4-nitrogen by young rice plants. Soil Sci. Plant Nutr. 24, 407–416.
Morgan M A and Jackson W A 1988a Suppression of ammonium uptake by nitrogen supply and its relief during nitrogen limitation. Physiol. Plant. 73, 38–45.
Morgan M A and Jackson W A 1988b Inward and outward movement of ammonium in root systems: Transient responses during recovery from nitrogen deprivation in presence of ammonium. J. Exp. Bot. 199, 179–191.
Morgan M A and Jackson W A 1989 Reciprocal ammonium transport into and out of plant roots: Modifications by plant nitrogen status and elevated root ammonium concentration. J. Exp. Bot. 211, 207–214.
Muller B and Touraine B 1992 Inhibition of NO3 uptake by various phloem translocated amino acids in soybean seedings. J. Exp. Bot. 43, 617–623.
Oji Y and Izawa G 1972 Quantitative changes of free amino acids and amides in barley plants during ammonia and nitrate assimilation. Plant Cell Physiol. 13, 249–259.
Pressland M R and McNaughton G S 1986 Whole plant studies using radioactive 13-Nitrogen. J. Exp. Bot. 184, 1619–1632.
Rodgers C O and Barneix A J 1988 Cultivar differences in the rate of nitrate uptake as releated to growth rate. Physiol. Plant. 72, 121–126.
Rodgers C O and Barneix A J 1989 The effect of N-deprivation on nitrate uptake and growth rate of two wheat cultivars selected for different fertility levels. Plant Physiol. Biochem. 27, 387–392.
Rodgers C O and Barneix A J 1992 The effect of amino acids and amides on the regulation of nitrate uptake by wheat seedlings. J. Pl. Nutr. 16. (In press).
Rufty T W, MacKown C T and Volk R J 1990 Alterations in nitrogen partioning in nitrogen stressed plants. Physiol. Plant. 79, 95–95.
Yemm E W and Cocking E C 1955 The determination of amino acids with ninhydrin. Analyst 80, 209–213.
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Causin, H.F., Barneix, A.J. Regulation of NH4 + uptake in wheat plants: Effect of root ammonium concentration and amino acids. Plant Soil 151, 211–218 (1993). https://doi.org/10.1007/BF00016286
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DOI: https://doi.org/10.1007/BF00016286