Abstract
Polyclonal antibodies against pea phytochrome detect 2 protein bands (about 116 and 120 kDa) on blots of crude protein extracts and protein of microsomal preparations of dark-grown tomato seedlings. Both protein bands are undetectable in Western blots of the aurea mutant extracts. Neither protein band is detectable after isogenic wild-type seedlings are illuminated with 3 h of red light, either in the crude extract or in the membrane fraction of the irradiated seedlings; this result is consistent with the hypothesis that both bands are phytochrome. When dark-grown wild-type seedlings are illuminated with 3 h of red light or blue light against a red light background, the transcript levels for chlorophyll a/b-binding proteins of photosystem I and II, plastocyanin, and the subunit II of photosystem I increase. In all cases, the same fluence rate of blue light is much more effective than red light alone, a result that indicates the involvement of a blue/UV-A light photoreceptor in addition to the involvement of the far-red-absorbing form of phytochrome, Pfr. The aurea mutant responds neither to red light nor to blue light. Thus, no Pfr-independent induction of the four transcripts by a blue/UV-A light photoreceptor can be measured in the aurea mutant.
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Abbreviations
- pb:
-
base pairs
- BL:
-
blue light
- cab-PSI:
-
chlorophyll a/b-binding protein of photosystem I
- cab-PSII:
-
chlorophyll a/b-binding protein of photosystem II
- Chl:
-
chlorophyll
- D:
-
darkness
- PC:
-
plastocyanin
- Pfr:
-
far-red light absorbing form of phytochrome, physiologically active
- Pr:
-
red light absorbing form of phytochrome, physiologically inactive
- rbcS:
-
small subunit of ribulose-1,5-bisphosphate carboxylase
- RL:
-
red light
- SDS:
-
sodium dodecyl sulfate
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Oelmüller, R., Kendrick, R.E. & Briggs, W.R. Blue-light mediated accumulation of nuclear-encoded transcripts coding for proteins of the thylakoid membrane is absent in the phytochrome-deficient aurea mutant of tomato. Plant Mol Biol 13, 223–232 (1989). https://doi.org/10.1007/BF00016140
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DOI: https://doi.org/10.1007/BF00016140