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Promoter for a Brassica napus ribulose bisphosphate carboxylase/oxygenase small subunit gene binds multiple nuclear factors and contains a negative-strand open reading frame encoding a putative transmembrane protein

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Abstract

Using a fractionated genomic bank, we have cloned and characterized a Brassica napus gene (rbcSF1) encoding the small sub-unit of ribulose 1,5-bisphosphate carboxylase. The promoter of this gene contains a 29 bp direct repeat capable of forming a single or a double hairpin loop, and three elements that are recognized by leaf nuclear proteins in vitro. The most upstream are the S-box, a small A/T-rich sequence between −516 and −512, and the F-box between −492 and −475. Finally, we have also observed binding to the G-box, a regulatory element common to numerous plant promoters. The promoter of rbcSF1 also has a 113 amino acids open reading frame (ORF113) in the non-coding strand. When used to probe a northern blot of leaf RNA, this ORF hybridizes to a 1.5 kb transcript. The protein encoded by ORF113 contains a transmembrane domain.

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Authors and Affiliations

  1. Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, G1K 7P4, Québec, Canada

    André M. Nantel, France Lafleur, Rodolphe Boivin & Guy Bellemare

  2. Allelix Crop Technologies, 6850 Goreway Drive, L4V 1P1, Mississauga, Ontario, Canada

    Chris L. Baszczynski

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  1. André M. Nantel
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  2. France Lafleur
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  3. Rodolphe Boivin
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  4. Chris L. Baszczynski
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  5. Guy Bellemare
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Nantel, A.M., Lafleur, F., Boivin, R. et al. Promoter for a Brassica napus ribulose bisphosphate carboxylase/oxygenase small subunit gene binds multiple nuclear factors and contains a negative-strand open reading frame encoding a putative transmembrane protein. Plant Mol Biol 16, 955–966 (1991). https://doi.org/10.1007/BF00016068

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  • DOI: https://doi.org/10.1007/BF00016068

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