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A model for the expression of CaMV nucleic acid

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Summary

Analysis of the sequence of CaMV full-length 35S transcript reveals two features which may relate to the translation of open regions I-V. There is a region in the 5′ leader sequence which could act as a ribosome binding site. This is followed by a sequence, which is complementary to sequences which are found just upstream to the open regions. The singificance of these sequences is discussed.

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References

  1. Al Ani R, Pfeiffer P, Whitechurch O, Lesot A, Lebeurier G, Hirth L: A virus specified protein produced upon infection of cauliflower mosaic virus. Ann Virol (Inst Pasteur) 131E:33–53, 1980.

    Google Scholar 

  2. Armour SL, Melcher U, Pirone TP, Lyttle DJ, Essenberg RC: Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30, 1983.

    Google Scholar 

  3. Balazs E, Guilley H, Jonard G, Richards K: Nucleotide sequence of DNA from altered-virulence isolate D/H of the cauliflower mosaic virus. Gene 19:239–249, 1982.

    Google Scholar 

  4. Baralle FE: The functional significance of leader and trailer sequences in eukaryotic mRNAs. Int Rev Cytol 81:71–106, 1983.

    Google Scholar 

  5. Cech TR: RNA splicing: three themes with variations. Cell 34:713–716, 1983.

    Google Scholar 

  6. Condit C, Hagan TJ, McKnight TD, Meagher RB: Characterization and preliminary mapping of cauliflower mosaic virus transcripts. Gene 25:101–108, 1983.

    Google Scholar 

  7. Covey SN, Hull R: Transcription of cauliflower mosaic virus DNA. Detection of transcripts, properties and location of gene encoding the virus inclusion body protein. Virology 111:463–474, 1981.

    Google Scholar 

  8. Covey SN, Lomonossoff GP, Hull R: Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts. Nucleic Acids Res 9: 6735–6747, 1981.

    Google Scholar 

  9. Darlix J-L, Spahr P-F: Binding sites of viral protein P19 onto Rous Sarcoma virus RNA and possible controls of viral functions. J Mol Biol 160:147–161, 1982.

    Google Scholar 

  10. Darlix J-L, Schwager M, Spahr P-F, Bromley PA: Analysis of the secondary and tertiary structures of Rous sarcoma virus RNA. Nucleic Acids Res 8:3335–3354, 1980.

    Google Scholar 

  11. Darlix J-L, Zuker M, Spahr P-F: Structure-function relationship of Rous sarcoma virus leader RNA. Nucleic Acids Res 10:5183–5196, 1982.

    Google Scholar 

  12. Daubert S, Richins R, Shepherd RJ, Gardner RC: Mapping of the coat protein gene of cauliflower mosaic virus by its expression in a prokaryotic system. Virology 122:444–449, 1982.

    Google Scholar 

  13. Dudley RK, Odell JT, Howell SH: Structure and 5′-termini of the large and 19S RNA transcripts encoded by the cauliflower mosaic virus genome. Virology 117:19–28, 1982.

    Google Scholar 

  14. Franck A, Guilley H, Jonard G, Richards K, Hirth L: Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–294, 1980.

    Google Scholar 

  15. Gardner RC, Howarth AJ, Hahn P, Brown-Luedi M, Shepherd RJ, Messing J: The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucleic Acids Res. 9:2871–2888, 1981.

    Google Scholar 

  16. Guilley H, Dudley RK, Jonard G, Balazs E, Richards KE: Transcription of cauliflower mosaic virus DNA: detection of promoter sequences and characterization of transcripts. Cell 30:763–773, 1982.

    Google Scholar 

  17. Hahn P, Shepherd RJ: Evidence for a 58-kilodalton polypeptide as precursor of the coat protein of cauliflower mosaic virus. Virology 116:480–488, 1982.

    Google Scholar 

  18. Hohn T, Richards K, Lebeurier G: Cauliflower mosaic virus on its way to becoming a useful plant vector. Current Topics in Microbiology and Immunology 96:193–236, 1981.

    Google Scholar 

  19. Hull R, Covey SN: Does cauliflower mosaic virus replicate by reverse transcription? Trends in Biochemical Sciences 8:119–121, 1983.

    Google Scholar 

  20. Hull R, Covey SN: Replication of cauliflower mosaic virus DNA. Sci Progr, Oxford 68:403–422, 1983.

    Google Scholar 

  21. Kozak M: How do eucaryotic ribosomes select initiation regions in messenger RNA?. Cell 15:1109–1123, 1978.

    Google Scholar 

  22. Kozak M: Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res 9:5233–5252, 1981.

    Google Scholar 

  23. Kozak M: Recognition of initiation sites in eukaryotic messenger RNAs. In: Perez-Bercoff R (ed) Protein biosynthesis in eukaryoties. Plenum Press, NY, 1982, pp 167–197.

    Google Scholar 

  24. Lockard RE, Connaught JF, Kumar A: Nucleotide sequence of the 5′ and 3′ domains for rabbit 18S ribsomal RNA. Nucleic Acids Res 10:3445–3457, 1982.

    Google Scholar 

  25. Odell JT, Howell SH: The identification, mapping and characterisation of mRNA for p66, a cauliflower mosaic viruscoded protein. Virology 102:349–359, 1980.

    Google Scholar 

  26. Odell JT, Dudley K, Howell SH: Structure of the 19S RNA transcript encoded by the cauliflower mosaic virus genome. Virology 111:377–385, 1981.

    Google Scholar 

  27. Pfeiffer P, Hohn T: Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–789, 1983.

    Google Scholar 

  28. Sieg K, Gronenborn B: Introduction and propagation of foreign DNA in plants using cauliflower mosaic virus as a vector. Abstr. NATO/FEBS Course Str Funct Plant Genomes p 154, 1982.

  29. Shine J, Dalgarno L: Determinant of cistron specificity in bacterial ribosomes. Nature, Lond 254:34–38, 1974.

    Google Scholar 

  30. Toh H, Hayashida H, Mijota T: Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus. Nature, Lon 305:827–829, 1983.

    Google Scholar 

  31. Varmus H, Swanstrom R: Replication of retroviruses. In: Weiss R, Teich N, Varmus H, Coffin J (eds) Molecular biology of tumor viruses. 2nd edn pt2, ‘RNA tumor viruses’. Cold Spring Harbor, NY 1982, pp 369–512.

  32. Woolston CJ, Covey SN, Penswick JR, Davies JW: Aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome. Gene 23: 15–23, 1983.

    Google Scholar 

  33. Xiong C, Muller S, Lebeurier G, Hirth L: Identification by immunoprecipitation of cauliflower mosaic virus in vitro major translation product with a specific serum against viroplasm protein. The EMBO Jour 1:971–976, 1982.

    Google Scholar 

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K.

    Roger Hull

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  1. Roger Hull
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Hull, R. A model for the expression of CaMV nucleic acid. Plant Mol Biol 3, 121–125 (1984). https://doi.org/10.1007/BF00016059

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  • DOI: https://doi.org/10.1007/BF00016059

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