Abstract
We have characterised a DNA-binding interaction common to the upstream sequences of the wheat fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) genes. The recognition site for this sequence-specific binding activity, designated wheat FBPase factor (WF-1), is located within 125 bp of the transcription start site of each gene. Within these regions there are no sequence motifs similar to those shown to be important for light-regulated expression in other species. The binding activity was not detected in wheat root nuclear extracts, or in pea leaf extracts. There was a higher level of binding activity in light-grown than in dark-grown wheat leaves. The level was also found to decline when light-grown plants were given an extended dark treatment, but could be reinduced by light. Utilising the gradient of developmental maturity which exists within the wheat leaf it was found that WF-1 activity increases during leaf development.
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References
Buchanan BB: Regulation of CO2 assimilation in oxygenic photosynthesis: the ferredoxin/thioredoxin system. Arch Biochem Biophys 288: 1–9 (1991).
Buzby JS, Yamada T, Tobin EM: A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter. Plant Cell 2: 805–814 (1990).
Cadet F, Meunier J-C: pH and kinetic studies of chloroplast sedoheptulose-1,7-bisphosphatase from spinach (Spinacia oleracea). Biochem J 253: 249–254 (1988).
Datta N, Cashmore AR: Binding of a pea nuclear protein to promoters of certain photoregulated genes is modulated by phosphorylation. Plant Cell 1: 1069–1077 (1989).
Dean C, van denElzen P, Tamaki S, Dunsmuir P, Bedbrook J: Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multigene family. EMBO J 4: 3055–3061 (1985).
Dean C, Pichersky E, Dunsmuir P: Structure, evolution, and regulation of RbcS genes in higher plants. Annu Rev Plant Physiol Plant Mol Biol 40: 415–439 (1989).
Gilmartin PM, Sarokin L, Memelink J, Chua N-H: Molecular light switches for plant genes. Plant Cell 2: 369–378 (1990).
Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA, Cashmore AR: An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci USA 85: 7089–7093 (1988).
Green PJ, Yong M-H, Cuozzo M, Kano-Murikami Y, Silverstein P, Chua N-H: Binding site requirements for the pea nuclear protein factor GT-1 correlate with sequences required for ligh-dependent transcriptional activation of the rbcS-3A gene. EMBO J 7: 4035–4044 (1988).
Green PJ, Kay SA, Lam E, Chua N-H: In vitro DNA footprinting. In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, Supplement 1, B 11: 1–22. Kluwer Academic Publishers, Dordrecht (1989).
Henikoff S: Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).
Kossmann J, Müller-Röber B, Dyer TA, Raines CA, Sonnewald U, Willmitzer L: Cloning and expression analysis of the plastidic fructose-1,6-bisphosphatase coding sequence from potato: circumstantial evidence for the import of hexoses into chloroplasts. Planta 188: 7–12 (1992).
Lloyd JC, Horsnell PR, Dyer TA, Raines CA: Structure and sequence of a wheat phosphoribulokinase gene. Plant Mol Biol 17: 167–168 (1991).
Lloyd JC, Raines CA, John UP, Dyer TA: The chloroplast FBPase gene of wheat: structure and expression of the promoter in photosynthetic and meristematic cells of transgenic tobacco plants. Mol Gen Genet 225: 209–216 (1991).
Manzara T, Gruissem W: Organisation and expression of the genes encoding ribulose-1,5-bisphosphate carboxylase in higher plants. Photosyn Res 16: 117–139 (1988).
Murphy GJP, Ward ES: Double-stranded DNA sequencing. In: Howe CJ, Ward ES (eds) Nucleic Acids Sequencing—A Practical Approach, pp. 99–115. IRL Press, Oxford (1990).
Raines CA, Lloyd JC, Longstaff M, Bradley D, Dyer TA: Chloroplast fructose-1,6-bisphosphatase: the product of a mosaic gene. Nucl Acids Res 16: 7931–7942 (1988).
Raines CA, Lloyd JC, Dyer TA: Molecular biology of the C3 photosynthetic carbon reduction cycle. Photosyn Res 27: 1–14 (1991).
Raines CA, Lloyd JC, Willingham NM, Potts S, Dyer TA: cDNA and gene sequences of wheat chloroplast sedoheptulose-1,7-bisphosphatase reveal homology with fructose-1,6-bisphosphatases. Eur J Biochem 205: 1053–1059 (1992).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, NY (1989).
Sarokin LP, Chua N-H: Binding sites of two novel phosphoproteins, 3AF5 and 3AF3, are required for rbcS-3A expression. Plant Cell 4: 473–483 (1992).
Schimkat D, Heineke D, Heldt HW: Regulation of sedoheptulose-1,7-bisphosphatase by sedoheptulose-7-phosphate and glycerate, and of fructose-1,6-bisphosphatase by glycerate in spinach chloroplasts. Planta 181: 97–103 (1990).
Shih M-C, Goodman HM: Differential light regulated expression of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenase in Nicotiana tabacum. EMBO J 7: 893–898 (1988).
Singh H, LeBowitz JH, Baldwin AS, Sharp PN: Molecular cloning of an enhancer binding protein: isolation by screening an expression library with recognition site DNA. Cell 52: 415–423 (1988).
Thompson WF, White MJ: Physiological and molecular studies of light-regulated nuclear genes in higher plants. Annu Rev Plant Physiol Plant Mol Biol 42: 423–466 (1991).
Tobin EM, Silverthorne J: Light regulation of gene expression in higher plants. Annu Rev Plant Physiol 36: 569–593 (1985).
Woodrow IE: Control of the rate of photosynthetic carbon dioxide fixation. Biochim Biophys Acta 851: 181–192 (1986).
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Miles, A.J., Potts, S.C., Willingham, N.M. et al. A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes. Plant Mol Biol 22, 507–516 (1993). https://doi.org/10.1007/BF00015979
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DOI: https://doi.org/10.1007/BF00015979