Summary
During the blue light-dependent chloroplast differentiation in suspension cultured cells of tobacco (Nicotiana tabacum var. ‘Samsun’) chlorophylls and other pigments as well as specific membrane and stroma proteins are synthesized de-novo: the 32-kD membrane protein of photosystem II, the small subunit (SSU) and the large subunit (LSU) of ribulose-bisphosphate carboxylase/oxygenase (EC. 4.1.1.39; RuBPCase). Parallel with their accumulation the steady-state concentration of the corresponding mRNAs increases rapidly and coordinately as was detected by employing cloned DNA sequences complementary to these plastid and nuclear transcripts as hybridization probes. The blue light-induced change in the concentration of the mRNAs analyzed here is abolished by red light when applied to cells at an early or late stage of chloroplast differentiation. The results indicate that blue light exerts a positive and coordinate influence on both genomes, nuclear and plastid, in chloroplast development of tobacco cells. re]19850308 rv]19850605 ac]19850627
Similar content being viewed by others
References
ApelK, KloppstechK: The plastid membranes of barley (Hordeum vulgare) Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a, b protein. Eur J Biochem 85:581–588, 1978.
BergmannL, BergerCH: Farblicht und Plastidendifferenzierung von Zellkulturen von Nicotiana tabacum var. Samsun. Planta 69:58–69, 1966.
GroßM, RichterG: Influence of sugars on blue light-induced synthesis of chlorophyll in cultured plant cells. Plant Cell Reports 1:288–290, 1982.
HandaAK, BressanRA, HandaS, HasegawaM: Clonal variation for tolerance to polyethylene glycol-induced water stress in cultured tomato cells. Plant Physiol 72:645–653, 1983.
LinkG: Cloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.). Nucleic Acids Res 9:3681–3694, 1981.
LinkG: Phytochrome control of plastid mRNA in mustard (Sinapis alba L.). Planta 154:81–86, 1982.
PinckL, FleckJ, PinckM, HaddaneR, HirthL: Sequence of a cDNA clone encoding part of the small subunit of ribulosebisphosphate carboxylase of Nicotiana silvestris. FEBS Lett 154:145–148, 1983.
RichterG: Blue light control of the level of two plastid mRNAs in cultured plant cells. Plant Mol Biol 3:271–276, 1984.
RichterG: Blue light effects on the level of translation and transcription. In: SengerH (ed) Blue light effects in biological systems. Springer, Berlin, Heidelberg, New York, 1984, pp 253–263.
RichterG, BeckmannJ, GroßM, HundrieserJ, SchneiderCh: Blue light-induced synthesis of chloroplast proteins in cultured plant cells. In: Progr Clinical a. Biol Res, Vol 102B-Cell function and differentiation, part B (FEBS Vol 65). Alan R Liss, New York, 1982, pp 267–276.
RichterG, HundrieserJ, GroßM, SchultzS, BottländerK, SchneiderCh: Blue light effects in cell cultures. In: SengerH (ed) Blue light effects in biological systems. Springer, Berlin, Heidelberg, New York, 1984, pp 387–396.
RodlandKD, RussellPJ: Simplified techniques for hybridization of DNA immobilized on GeneScreen and Gene-Screen-Plus, using 32P-labeled or 35S-labeled DNA probes. New Prod News 2:1–6, 1983.
SasakiY, SakihamaT, KamikuboT, ShinozakiK: Phytochrome-mediated regulation of two mRNAs, encoded by nuclei and chloroplasts, of ribulosebisphosphate carboxylase/oxygenase. Eur J Biochem 257:8569–8572, 1983.
SouthernEM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517, 1975.
StiekemaWJ, WimpeeChF, SilverthorneJ, TobinEM: Phytochrome control of the expression of two nuclear genes encoding chloroplast proteins in Lemna gibba L. G-3. Plant Physiol 72:717–724, 1983.
WhiteBA, BancroftFC: Cytoplasmic dot hybridization. J Biol Chem 257:8569–8572, 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Richter, G., Wessel, K. Red light inhibits blue light-induced chloroplast development in cultured plant cells at the mRNA level. Plant Mol Biol 5, 175–182 (1985). https://doi.org/10.1007/BF00015681
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00015681