Skip to main content
SpringerLink
Log in

Organisation and expression of a wound/ripening-related small multigene family from tomato

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

cDNA clones derived from a ripe tomato fruit cDNA library were used to investigate changes in the abundance of specific mRNAs in ripening fruit and wounded leaves. mRNAs related to one cDNA clone (pTOM 13) were expressed in both situations. This clone was used to identify homologous sequences in a tomato genomic library. Three groups of related clones that hybridised to the pTOM 13 cDNA insert were identified and subcloned into plasmid vectors. Genomic Southern analysis of tomato DNA using gene-specific DNA fragments isolated from the subcloned DNAs indicated that all pTOM 13 closely related genes had been isolated. RNA dot blot analysis with these DNA fragments as probes indicated differential expression of this small multigene family in leaves and fruit.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Acaster MA, Kende H: Properties and partial purification of 1-amino-cyclopropane-1-carboxylate synthase. Plant Physiol 72: 139–145 (1983).

    Google Scholar 

  2. Adams DO, Yang SF: Ethylene biosynthesis: identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate of the conversion of methionine to ethylene. Proc Natl Acad Sci USA 76: 170–174 (1979).

    Google Scholar 

  3. Aviv H, Leder P: Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci USA 69: 1408–1412 (1972).

    Google Scholar 

  4. Birnboim HC, Doly J: A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7: 1513–1523 (1979).

    Google Scholar 

  5. Boller T, Gehri A, Mauch F, Vogeli U: Chitinase in bean leaves: induction by ethylene, purification, and possible function. Planta 157: 22–31 (1983).

    Google Scholar 

  6. Boller T, Kende H: Regulation of wound ethylene synthesis in plants. Nature 286: 259–260 (1980).

    Google Scholar 

  7. Broglie KE, Gaynor JJ, Broglie RM: Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Natl Acad Sci USA 83: 6820–6824 (1986).

    Google Scholar 

  8. Chalutz E: Ethylene-induced phenylalanine ammonia lyase activity in carrot roots. Plant Physiol 51: 1033–1036 (1973).

    Google Scholar 

  9. Christoffersen RE, Laties GG: Ethylene regulated gene expression in carrots. Proc Natl Acad Sci USA 79: 4060–4063 (1982).

    Google Scholar 

  10. Dellaporta SL, Wood J, Hicks JB: A plant DNA mini preparation: Version 2. Plant Mol Biol Rep 1: 19–21 (1983).

    Google Scholar 

  11. Frischauf A, Lehrach H, Poustka A, Murray N: Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842 (1983).

    Google Scholar 

  12. Grierson D, Maunders MJ, Slater A, Ray J, Bird CR, Schuch W, Holdsworth MJ, Tucker GA, Knapp JE: Gene expression during tomato ripening. Phil Trans R Soc Lond B314: 399–410 (1986).

    Google Scholar 

  13. Grierson D, Slater A, Speirs J, Tucker GA: The appearance of polygalacturonase mRNA in tomatoes: one of a series of changes in gene expression during development and ripening. Planta 163: 263–271 (1985).

    Google Scholar 

  14. Grierson D, Tucker GA: Timing of ethylene and polygalacturonase synthesis in relation to the control of tomato fruit ripening. Planta 157: 174–179 (1983).

    Google Scholar 

  15. Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W: Sequencing and identification of a cDNA clone for tomato polygalacturonase. Nucl Acids Res 14: 8595–8603 (1986).

    Google Scholar 

  16. Hightower RC, Meagher RB: Divergence and differential expression of soybean actin genes. EMBO J 4: 1–8 (1985).

    Google Scholar 

  17. Holdsworth MJ, Bird CR, Ray J, Schuch W, Grierson D: Structure and expression of an ethylene-related mRNA from tomato. Nucl Acids Res 15: 731–739 (1987).

    Google Scholar 

  18. Jeffery D, Smith C, Goodenough P, Prosser I, Grierson D: Ethylene independent and ethylene dependent biochemical changes in ripening tomatoes. Plant Physiol 74: 32–38 (1984).

    Google Scholar 

  19. Kelly P, Trewavas AJ, Lewis LN, Durbin ML, Sexton R: Translatable mRNA changes in ethylene induced abscission zones of Phaseolus vulgaris (Red Kidney). Plant Cell and Env 10: 11–16 (1987).

    Google Scholar 

  20. Kende H, Boller T: Wound ethylene and 1-aminocyclopropane-1-carboxylate synthase in ripening tomato fruit. Planta 151: 476–481 (1981).

    Google Scholar 

  21. Konze JR, Kwiatkowski GMK: Rapidly induced ethylene formation after wounding is controlled by the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis. Planta 151: 327–330 (1981).

    Google Scholar 

  22. Leiberman M: Biosynthesis and action of ethylene. Ann Rev Plant Physiol 30: 533–591 (1979).

    Google Scholar 

  23. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

    Google Scholar 

  24. Maunders MJ, Holdsworth MJ, Slater A, Knapp JE, Bird CR, Schuch W, Grierson D: Ethylene stimulates the accumulation of ripening related mRNAs in tomatoes. Plant Cell and Env 10: 177–184 (1987).

    Google Scholar 

  25. Messing J, Vieira J: A new pair of M13 vectors for selecting either DNA strand of double digest restriction fragments. Gene 19: 269–276 (1982).

    Google Scholar 

  26. Nichols SE, Laties GG: Ethylene-regulated gene transcription in carrot roots. Plant Mol Biol 3: 393–401 (1984).

    Google Scholar 

  27. Rigby PWJ, Dieckmann M, Rhodes SC, Berg P: Labelling of deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Mol Biol 113: 237–251 (1977).

    Google Scholar 

  28. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    Google Scholar 

  29. Sitrit Y, Riov J, Blumenfeld A: Regulation of ethylene biosynthesis in avocado during ripening. Plant Physiol 81: 130–135 (1986).

    Google Scholar 

  30. Slater A, Maunders MJ, Edwards K, Schuch W, Grierson D: Isolation and characterisation of cDNA clones for tomato polygalacturonase and other ripening related proteins. Plant Mol Biol 5: 137–147 (1985).

    Google Scholar 

  31. Smith CJS, Slater A, Grierson D: Rapid appearance of an mRNA correlated with ethylene synthesis encoding a protein of molecular weight 35000. Planta 168: 94–100 (1986).

    Google Scholar 

  32. Stevens M, Rick CM: Genetics in breeding. In: Atherton JG, Rudich R (eds) The Tomato Crop, pp 35–109. Chapman and Hall, London (1986).

    Google Scholar 

  33. Theologis A, Laties GG: Potentiating effect of pure oxygen on the enhancement of respiration by ethylene in plant storage organs: A comparative study: Plant Physiol 69: 1031–1035 (1982).

    Google Scholar 

  34. Thomas P: Hybridisation of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205 (1980).

    Google Scholar 

  35. Tomes ML: Temperature inhibition of carotene synthesis in tomato. Bot Gaz 124: 180–185 (1963).

    Google Scholar 

  36. Tucker GA, Brady CJ: Silver ions interrupt tomato fruit ripening. J Plant Physiol 127: 165–169 (1987).

    Google Scholar 

  37. Vieira J, Messing J: The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259–268 (1982).

    Google Scholar 

  38. Yang SF, Hoffman NE: Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol 35: 155–189 (1984).

    Google Scholar 

  39. Zurfluh LL, Guilfoyle TJ: Auxin and ethylene-induced changes in the population of translatable messenger RNA in basal sections and intact soybean hypocotyls. Plant Physiol 69: 338–340 (1982).

    Google Scholar 

Download references

Author information

Author notes

  1. Michael J. Holdsworth

    Present address: Department of Biology, UCLA, 405 Hilgard Avenue, Los Angeles, California, USA

Authors and Affiliations

  1. Department of Physiology and Environmental Science, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, UK

    Michael J. Holdsworth & Donald Grierson

  2. ICI Plant Biotechnology Group, The Heath, P.O. Box 11, WA7 4QE, Runcorn, Cheshire, UK

    Wolfgang Schuch

Authors
  1. Michael J. Holdsworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wolfgang Schuch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Donald Grierson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Holdsworth, M.J., Schuch, W. & Grierson, D. Organisation and expression of a wound/ripening-related small multigene family from tomato. Plant Mol Biol 11, 81–88 (1988). https://doi.org/10.1007/BF00015661

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00015661

Key words

Search

Navigation