Abstract
the entire mitochondrial genome (mt genome) of the unicellular green alga Platymonas subcordiformis (synonym Tetraselmis subcordiformis; Prasinophyceae) was cloned and a physical map for the four restriction enzymes Hind III, Eco RI, Bgl II and Xba I was constructed. The mt genome of P. subcordiformis is a 42.8 kb circular molecule, coding for at least 23 genes. Hybridization and sequence analysis revealed the presence of a ca. 1.5 kb inverted repeat on the mt genome of P. subcordiformis. Phylogenetic analyses based on sequences of several coxI genes were carried out. Our data indicate that mitochondria from P. subcordiformis and from land plants form a natural, monophyletic group.
References
Bibb MB, vanEtten RA, Wright CT, Walberg MW, Clayton DA: Sequence and gene organization of mouse mitochondrial DNA. Cell 26: 167–180 (1981).
Boyen C, Leblanc C, Kloareg B, Loiseaux-de Goër S: Physical map and gene organization of the mitochondrial genome of Chondrus crispus (Rhodophyta, Gigartinales). Plant Mol Biol 26: 691–697 (1994).
Boyen C, Leblanc C, Bonnard G, Grienenberger JM, Kloareg B: Nucleotide sequence of the cox3 gene from Chondrus crispus: evidence that UGA encodes tryptophan and evolutionary implications. Nucl Acids Res 22: 1400–1403 (1994).
Chapman RL, Buchheim MA: Green algae and the evolution of land plants: inferences from nuclear-encoded rRNA gene sequences. BioSystems 28: 127–137 (1992).
Clark-Walker GD: Evolution of mitochondrial genomes in fungi. In: Wolstenholme DR, Jeon KW (eds) Mitochondrial Genome, pp. 89–127. Academic Press, San Diego (1992).
Coleman AW, Goff LJ: DNA analysis of eukaryotic algal species. J Phycol 27: 463–473 (1991).
Devereux R, LoeblichIII AR, Fox GE: Higher plant origins and the phylogeny of green algae. J Mol Evol 31: 18–24 (1990).
Gray MW: The endosymbiont hypothesis revisited. In: Wolstenholme DR, Jeon KW (eds) Mitochondrial Genome, pp. 233–357. Academic Press, San Diego (1992).
Hanson MR, Folkerts O: Structure and function of the higher plant mitochondrial genome. In: Wolstenholme DR, Jeon KW (eds) Mitochondrial Genome, pp. 129–172. Academic Press, San Diego (1992).
Higgins DG, Beasby AJ, Fuchs R: Clustal V: improved software for multiple sequence alignment. CABIOS 8: 189–191 (1992).
Hintz WE, Mohan M, Anderson JB, Horgen PA: The mitochondrial DNAs of Agaricus: heterogeneity in A. bitorquis and homogeneity in A. brunnescens. Curr Genet 9: 127–132 (1985).
Lang BF, Ahne F, Bonen L: The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: the cytochrome b gene has an intron closely related to the first two introns in the Saccharomyces cerevisiae coxI gene. J Mol Biol 184: 353–366 (1985).
Lonsdale DM, Hodge TP, Howe CJ, Stern DB: Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell 34: 1007–1014 (1983).
Michaelis G, Vahrenholz C, Pratje E: Mitochondrial DNA of Chlamydomonas reinhardtii: the gene for apocytochrome b and the complete functional map of the 15.8 kb DNA. Mol Gen Genet 223: 211–216 (1990).
Nozato N, Oda K, Yamato K, Ohta E, Takemura M, Akashi K, Fukuzawa H, Ohyama K: Cotranscriptional expression of mitochondrial genes for subunits of NADH dehydrogenase, nad5, nad4, nad2, in Marchantia polymorpha. Mol Gen Genet 237: 343–350 (1993).
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K: Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223: 1–7 (1992).
Palmer JD: Comparison of chloroplast and mitochondrial genome evolution in plants. In: Hermann RG (eds) Cell Organelles, pp. 99–133. Springer-Verlag, Wien/New York (1992).
Palmer JD, Shields CR: Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307: 437–440 (1984).
Saitou N, Nei M: The neigbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 (1987).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).
Schuster W, Brennicke A: Interorganeller sequence transfer: plant mirochondrial DNA is nuclear, is plastid, is mitochondrial. Plant Sci 54: 1–10 (1988).
Schuster W, Brennicke A: The plant mitochondrial genome: physical structure, information content, RNA editing, and gene migration to the nucleus. Annu Rev Plant Physiol Plant Mol Biol 45: 61–78 (1994).
Stern DB, Palmer JD: Tripartite mitochondrial genome of spinach: physical structure, mitochondrial gene mapping, and locations of transposed chloroplast DNA sequences. Nucl Acids Res 14: 5651–5666 (1986).
Stern DB, Lonsdale DM: Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299: 698–702 (1982).
Vahrenholz C, Pratje E, Michaelis G, Dujon B: Mitochondrial DNA of Chlamydomonas reinhardtii: sequence and arrangement of URF5 and the gene for cytochrome oxidase subunit I. Mol Gen Genet 201: 213–224 (1985).
van denHoek C, Jahns HM, Mann DG: Algen. Thieme Verlag, Stuttgart/New York (1993).
van dePeer Y, deWachter R: TREECON: a software package for the construction and drawing of evolutionary trees. Comput Applic Biosci 9: 177–182 (1993).
Ward BL, Anderson RS, Bendich AJ: The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae). Cell 25: 793–803 (1981).
Wolff G, Kück U: Organization and coding capacity of mitochondrial genomes of algae. In: Brennicke A, Kück U (eds) Plant Mitochondria, pp. 103–113. VCH Chemie, Weinheim (1993).
Wolff G, Plante I, Lang BF, Kück U, Burger G: Complete sequence of the mitochondrial DNA of the chlorophyte alga Prototheca wickerhamii. J Mol Biol 237: 75–86 (1994).
Wolstenholme DR: Animal mitochondrial DNA: structure and evolution. In: Wolstenholme DR, Jeon KW (eds) Mitochondrial Genome, pp. 173–216. Academic Press, San Diego (1992).
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Kessler, U., Zetsche, K. Physical map and gene organization of the mitochondrial genome from the unicellular green alga Platymonas (Tetraselmis) subcordiformis (Prasinophyceae) . Plant Mol Biol 29, 1081–1086 (1995). https://doi.org/10.1007/BF00014979
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DOI: https://doi.org/10.1007/BF00014979