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Carotenoids in photosynthesis: Protection of D1 degradation in the light

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Abstract

Photosynthesis has been determined with mutants of Anacystis which form different amounts of carotenoids. With these cultures a highly significant correlation between photosynthetic oxygen evolution and the amounts of synthesized carotenoids was observed. In addition, the influence of carotenoids on light-dependent degradation of thylakoid proteins was investigated with Scenedesmus cultures grown in darkness in the presence of norflurazon, an inhibitor of carotenoid biosynthesis. Pre-illumination of cells resulted in decrease of photosynthetic activity accompanied by loss of the D1 protein. This effect is dependent on the length of illumination, and the light intensity, and increased when carotenoid content was lowered during previous growth of the norflurazon-treated cultures.

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Abbreviations

BSA:

bovine serum albumin

D1:

32 kDa QB-binding protein

EDTA:

ethylenediaminetetraacetic acid

LHCII:

light-harvesting complex II

PMSF:

phenylmethylsulfonyl fluoride

PS:

photosystem

tricine:

N-[tris(hydroxymethyl)methyl] glycine

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Authors and Affiliations

  1. Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, P.O. Box 5560, D-7750, Konstanz, Germany

    Gerhard Sandmann, Matthias Kuhn & Peter Böger

Authors
  1. Gerhard Sandmann
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  2. Matthias Kuhn
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  3. Peter Böger
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Sandmann, G., Kuhn, M. & Böger, P. Carotenoids in photosynthesis: Protection of D1 degradation in the light. Photosynth Res 35, 185–190 (1993). https://doi.org/10.1007/BF00014749

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  • DOI: https://doi.org/10.1007/BF00014749

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