Abstract
Methods have been evaluated and standardised to measure the phosphatase, saccharase, amylase and lipase enzymatic activities in aquatic environments. The analytical reproducibility, the ecological applicability and the etiology of these activities have been investigated. The usefulness of the various enzymatic assays for routine analyses and for fundamental research of aquatic environments is discussed.
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De Maeseneer, J. 1972. Limnological observations on the ‘Nationale Watersportbaan Georges Nachez’ at Ghent in 1968, 1969, 1970 and 1971. Med. v. d. Fac. Landbouwwetenschappen R.U.G. 37(1): 289–313.
Dobbs, R. A. & Williams, R. T. 1963. Elimination of chloride interference in the Chemical Oxygen Demand test. Anal. Chem. 35 (8): 1064–1067.
Hoffmann, G. & Dedeken, M. 1965. Eine Methode zur kolorimetrischen Bestimmung der β-Glucosidaseaktivität in Böden. Z. Pfl. Ernähr. Düng. Bodenk. 108: 193–198.
Hofmann, E. D. & Hoffmann, G. G. 1966. Die Bestimmung der biologischen Tätigkeit in Böden mit Enzymenmethoden. Adv. Enzymology 28: 365–390.
Jones, J. G. 1970. A medium for freshwater bacteria. J. appl. Bact. 33: 679–686.
Jones, J. G. 1971. Studies on freshwater bacteria: factors which influence the population and itsactivity. J.Ecol. 59 (2): 593–613.
Jones, J. G. 1972a. Studies on freshwater bacteria: association with algae and alkaline phosphatase activity. J. Ecol. 60(1): 54–75.
Jones, J. G. 1972b. Studies on freshwater microorganisms: phosphatase activity in lakes of differing degrees of eutrofication. J. Ecol. 60 (3): 777–791.
Jones, J. G. 1973. Use of nonparametric tests for the analysis of data obtained from preliminary surveys: a review. J. appl. Bact. 36: 197–210.
Lawrence, R. C., Fryer, T. F. & Reiter, B. 1967. The production and characterization of lipase from a Micrococcus and a Pseudomonas. J. Gen. Microbiol. 48: 401–418.
Lenhard, G. 1967. Determination of protease activity in bottom deposits of sewage stabilization ponds. Hydrobiologia 29: 67–79.
Lenhard, G. 1968. A standardized procedure for the determination of dehydrogenase activity in samples from anaerobic treatment systems. Water Res. 2: 161–167.
Overbeck, J. & Babenzien, H. D. 1964. Über den Nachweis von freien enzymen in Gewässer. Arch. Hydrobiol. 60: 107–114.
Reichardt, W. 1971. Catalytic mobilization of phosphate in lake water and by Cyanophyta. Hydrobiologia 38: 377–394.
Richardt, W., Overbeck, J. & Steubing, L. 1967. Free dissolved enzymes in lake waters. Nature 216: 1345–1347.
Schwoerbel, J. 1972. Methods of hydrobiology. Pergamon Press.
Smith, W. & Roe, J. H. 1949. A photometric method for the determination of α-amylase in blood and urine, with use of the starch-iodine color. J. Biol. Chem. 179: 53–59.
Sridhar, M. K. C. & Pillai, S. C. 1969. Catalase activity in polluted waters. Effl. Water. Treat. J. 9: 81.
Standard methods for the examination of water and wastewater. 1971. (13th edit.) American Public Health Association, 1015 18th St., N.W., Washington D.C. 20036 USA.
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Verstraete, W., Voets, J.P. & van Lancker, P. Evaluation of some enzymatic methods to measure the bio-activity of aquatic environments. Hydrobiologia 49, 257–266 (1976). https://doi.org/10.1007/BF00014519
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DOI: https://doi.org/10.1007/BF00014519