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cDNA cloning and gene expression of the major prolamins of rice

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Abstract

A full-length cDNA (pS 18) encoding the 16 kDa rice prolamin composed of 158 amino acids was sequenced. Analysis of N-terminal amino acid sequence of a major rice prolamin indicated that an 18 amino acid signal peptide was removed from 16 kDa precursor prolamin to form the 14 kDa prolamin during seed development. Synthesis of the 16 kDa precursor prolamin began around 8 days after flowering (DAF), increased remarkably at 8–11 DAF and gradually reached maximum levels with the maturation of rice seeds.

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References

  1. Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J: mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucl Acids Res 14: 2229–2240 (1986).

    Google Scholar 

  2. Kim WT, Okita TW: Structure, expression, and heterogeneity of the rice seed prolamins. Plant Physiol 88: 649–655 (1988).

    Google Scholar 

  3. Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).

    Google Scholar 

  4. Mandac BE, Juliano BO: Properties of prolamin in mature and developing rice grain. Phytochemistry 17: 611–614 (1978).

    Google Scholar 

  5. Maniatis T, Fritsch EF, Sambrook J: Gel electrophoresis of RNA. In: Molecular Cloning: A Laboratory Manual, pp. 199–201. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).

    Google Scholar 

  6. Masumura T, Hibino T, Kidzu K, Mitsukawa N, Tanaka K, Fujii S: Cloning and characterization of a cDNA encoding a rice 13 kDa prolamin. Mol Gen Genet 221: 1–7 (1990).

    Google Scholar 

  7. Masumura T, Shibata D, Hibino T, Kato T, Kawabe K, Takeba G, Tanaka K, Fujii S: cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds. Plant Mol Biol 12: 123–130 (1989).

    Google Scholar 

  8. O'Farrell PZ, Goodman HM, O'Farrell PH: High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133–1142 (1977).

    Google Scholar 

  9. Padhye VW, Salunkhe DK: Extraction and characterization of rice proteins. Cereal Chem 56: 389–393 (1979).

    Google Scholar 

  10. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    Google Scholar 

  11. Shyur LF, Chen CS: Nucleotide sequence of two rice prolamin cDNAs. Nucl Acids Res 18: 6683 (1990).

    Google Scholar 

  12. Sugimoto T, Tanaka K, Kasai Z: Improved extraction of rice prolamin. Agric Biol Chem 50: 2409–2411 (1986).

    Google Scholar 

  13. Sugiyama T, Rafalski JA, Söll D: The nucleotide sequence of a wheat γ-gliadin genomic clone. Plant Sci 44: 205–209 (1986).

    Google Scholar 

  14. Sumner-Smith M, Rafalski JA, Sugiyama T, Stoll M, Söll D: Conservation and variability of wheat α/β-gliadin genes. Nucl Acids Res 13: 3905–3916 (1985).

    Google Scholar 

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Shyur, LF., Wen, TN. & Chen, CS. cDNA cloning and gene expression of the major prolamins of rice. Plant Mol Biol 20, 323–326 (1992). https://doi.org/10.1007/BF00014501

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  • DOI: https://doi.org/10.1007/BF00014501

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