Abstract
A full-length cDNA (pS 18) encoding the 16 kDa rice prolamin composed of 158 amino acids was sequenced. Analysis of N-terminal amino acid sequence of a major rice prolamin indicated that an 18 amino acid signal peptide was removed from 16 kDa precursor prolamin to form the 14 kDa prolamin during seed development. Synthesis of the 16 kDa precursor prolamin began around 8 days after flowering (DAF), increased remarkably at 8–11 DAF and gradually reached maximum levels with the maturation of rice seeds.
References
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J: mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucl Acids Res 14: 2229–2240 (1986).
Kim WT, Okita TW: Structure, expression, and heterogeneity of the rice seed prolamins. Plant Physiol 88: 649–655 (1988).
Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Mandac BE, Juliano BO: Properties of prolamin in mature and developing rice grain. Phytochemistry 17: 611–614 (1978).
Maniatis T, Fritsch EF, Sambrook J: Gel electrophoresis of RNA. In: Molecular Cloning: A Laboratory Manual, pp. 199–201. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Masumura T, Hibino T, Kidzu K, Mitsukawa N, Tanaka K, Fujii S: Cloning and characterization of a cDNA encoding a rice 13 kDa prolamin. Mol Gen Genet 221: 1–7 (1990).
Masumura T, Shibata D, Hibino T, Kato T, Kawabe K, Takeba G, Tanaka K, Fujii S: cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds. Plant Mol Biol 12: 123–130 (1989).
O'Farrell PZ, Goodman HM, O'Farrell PH: High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133–1142 (1977).
Padhye VW, Salunkhe DK: Extraction and characterization of rice proteins. Cereal Chem 56: 389–393 (1979).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Shyur LF, Chen CS: Nucleotide sequence of two rice prolamin cDNAs. Nucl Acids Res 18: 6683 (1990).
Sugimoto T, Tanaka K, Kasai Z: Improved extraction of rice prolamin. Agric Biol Chem 50: 2409–2411 (1986).
Sugiyama T, Rafalski JA, Söll D: The nucleotide sequence of a wheat γ-gliadin genomic clone. Plant Sci 44: 205–209 (1986).
Sumner-Smith M, Rafalski JA, Sugiyama T, Stoll M, Söll D: Conservation and variability of wheat α/β-gliadin genes. Nucl Acids Res 13: 3905–3916 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shyur, LF., Wen, TN. & Chen, CS. cDNA cloning and gene expression of the major prolamins of rice. Plant Mol Biol 20, 323–326 (1992). https://doi.org/10.1007/BF00014501
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00014501