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Influence of three vesicular-arbuscular mycorrhizal fungi (Glomaceae) on the activity of specific enzymes in the root system of Cucumis sativus L.

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Abstract

The influence of three vesicular-arbuscular mycorrhizal (VAM) Glomus species on the activity of enzymes in the roots of Cucumis sativus was tested. Cucumber plants were grown in a split-root system, in which colonized and uncolonized roots of a single plant could be separated. The activity of the host root malate dehydrogenase (MDH), glucose 6-phosphate dehydrogenase (Gd), glutamate oxaloacetate transaminase (GOT) and glutamate dehydrogenase (GDH) was measured on a densitometer after separation of the host and fungal enzymes on polyacrylamide gels.

The results showed that only minor changes in the activity of the host root enzymes occurred after VAM inoculation. Gd was stimulated by VAM and phosphorus, and one of the fungi decreased the activity of GDH in the host plant when both parts of the root system were colonized.

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References

  • Abbott L K and Robson A D 1984 The effect of mycorrhizas on plant growth. In VA Mycorrhizae. Eds. C L Powell and D J Bagyaraj. pp 113–130. CRC Press, Boca Raton, FL.

    Google Scholar 

  • Blair D A, Peterson R L and Bowley S R 1988 Nuclear DNA content in root cells of Lotus and Trifolium colonized by the VAM fungus Glomus versiforme. New Phytol. 109, 167–170.

    Google Scholar 

  • Bollard E G and Matthews R E F 1966 The physiology of parasitic disease. In Plant Physiology. A Treatise. Vol. IVB: Metabolism: Intermediary Metabolism and Pathology. Eds. F C Steward. pp 417–550. Academic Press, New York, London.

    Google Scholar 

  • Capaccio C M and Callow J A 1982 The enzymes of polyphosphate metabolism in vesicular-arbuscular mycorrhizas. New Phytol. 91, 81–91.

    Google Scholar 

  • Carling D E, Riehle W G, Brown M F and Johnson D R 1978 Effects of a vesicular-arbuscular mycorrhizal fungus on nitrate reductase and nitrogenase activities in nodulating and non-nodulating soybeans. Phytopathology 68, 1590–1596.

    Google Scholar 

  • Dehne H W 1986 Influence of VA mycorrhizae on the host plant physiology. In Mycorrhizae: Physiology and Genetics. Eds. V Gianinazzi and S Gianinazzi. pp 431–435. INRA, Dijon, France.

    Google Scholar 

  • Dehne H W 1987 Zur Nutzung der VA Mykorrhiza als Antistressfaktor. Angew. Bot. 61, 135–143.

    Google Scholar 

  • Dehne H W, Schönbeck F and Baltruschat H 1978 The influence of endotrophic mycorrhiza on plant diseases. 3. Chitinase-activity and ornithine-cycle. Z. Pflanzenkr. Pflanzenschutz 85, 666–678.

    Google Scholar 

  • Dehne H W and Schönbeck F 1979 Unterschungen zum Einfluss der endotrophen Mykorrhiza auf Pflanzenkrankheiten. II. Phenolstoffwechsel and Lignifizierung. Phytopath. Z. 95, 210–216.

    Google Scholar 

  • Dodd J C, Burton C C, Burns R G and Jeffries P 1987 Phosphatase activity associated with the roots and the rhizosphere of plants infected with vesicular-arbuscular mycorrhizal fungi. New Phytol. 107, 163–172.

    Google Scholar 

  • Gianinazzi-Pearson V and Gianinazzi S 1976 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. I. Effect of mycorrhiza formation and phosphorus nutrition on soluble phosphatase activities in onion roots. Physiol. Veg. 14, 833–841.

    Google Scholar 

  • Gianinazzi-Pearson V and Gianinazzi S 1978 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. II. Soluble alkaline phosphatase specific to mycorrhizal infection in onion roots. Physiol. Pl. Pathol. 12, 45–53.

    Google Scholar 

  • Harris H and Hopkinson D A 1978 Handbook of Enzyme Electrophoresis in Human Genetics (with supplements). Oxford American Publishing Co., New York.

    Google Scholar 

  • Hepper C M 1977 A colorimetric method for estimating vesicular-arbuscular mycorrhizal infection in roots. Soil Biol. Biochem. 9, 15–18.

    Google Scholar 

  • Hepper C M, Sen R and Maskall C S 1986 Identification of vesicular-arbuscular mycorrhizal fungi in roots of leek (Allium porrum L.) and maize (Zea mays L.) on the basis of enzyme mobility during polyacrylamide gel electrophoresis. New Phytol. 102, 529–539.

    Google Scholar 

  • Krishna K R, Bagyaraj D J and Papavinsasundaram K G 1983 Acid and alkaline phosphatase activities in mycorrhizal and uninfected roots of Arachis hypogaea L. Ann. Bot. 51, 551–553.

    Google Scholar 

  • Lowry O H, Rosenburg N J, Farr A L and Randall R J 1951 Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • McGee P A and Smith S E 1990 Activity of succinate dehydrogenase in vesicular-arbuscular mycorrhizal fungi after enzymic digestion from roots of Allium porrum. Mycol. Res. 94, 305–308.

    Google Scholar 

  • Miflin B J and Lea P J 1977 Amino acid metabolism. Annu. Rev. Plant Physiol. 28, 299–329.

    Google Scholar 

  • Oliver A J, Smith S E, Nicholas D J D, Wallace W W and Smith F A 1983 Activity of nitrate reductase in Trifolium subterraneum: Effects of mycorrhizal infection and phosphate nutrition. New Phytol. 94, 63–79.

    Google Scholar 

  • Reddy M N and Stahmann M A 1975 Malate dehydrogenase in the Fusarial wilt disease of peas. Physiol. Plant Pathol. 7, 99–111.

    Google Scholar 

  • Rosendahl S 1989 Comparisons of spore-cluster forming Glomus species (Endogonaceae) based on morphological characteristics and isoenzyme banding patterns. Opera Bot. 100, 215–223.

    Google Scholar 

  • Rosendahl S, Sen R, Hepper C M and Azcon-Aguilar C 1989 Quantification of three vesicular-arbuscular mycorrhizal fungi (Glomus spp.) in roots of leek (Allium porrum) on the basis of activity of diagnostic enzymes after polyacrylamide gel electrophoresis. Soil Biol. Biochem 21, 519–522.

    Google Scholar 

  • Sen R and Hepper C M 1986 Characterization of vesicular-arbuscular mycorrhizal fungi (Glomus spp.) by selective enzyme staining following polyacrylamide gel electrophoresis. Soil Biol. Biochem. 18, 29–34.

    Google Scholar 

  • Smith S E, StJohn B J, Smith F A and Nicholas D J D 1985 Activity of glutamine synthetase and glutamate dehydrogenase in Trifolium subterraneum L. and Allium cepa L.: Effects of mycorrhizal infection and phosphate nutrition. New Phytol. 99, 211–227.

    Google Scholar 

  • Tanksley S D and Orton T J 1983 Isozymes in plant genetics and breeding, Part A. Elsevier, Amsterdam, Oxford, New York.

    Google Scholar 

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Rosendahl, S. Influence of three vesicular-arbuscular mycorrhizal fungi (Glomaceae) on the activity of specific enzymes in the root system of Cucumis sativus L.. Plant Soil 144, 219–226 (1992). https://doi.org/10.1007/BF00012878

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