Abstract
Cryo-microprobe analysis of quench-frozen fronds of a Zn-tolerant clone of Lemna minor exposed to a high level of Zn (300 μM) showed the presence of cellular deposits consisting of Zn, Mg, K and P or Zn, K and P (Zn phytate). The same Zn-tolerant clone of Lemna minor, when exposed to a high level of Cd (30 μM), showed the presence of globular deposits consisting of Cd, K and P in mature fronds, but the immature cells of the enclosed daughter fronds contained relatively large deposits with Cd and S as the main components (Cd-phytochelatin?). Selection for Zn tolerance in a population of Lemna minor was easily achieved but selection for Cd tolerance has so far not been successful. The Zn-tolerant clone also tolerates high levels of phosphate.
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References
Alexander T G and Robertson J A 1970 Ascorbic acid as a reductant for inorganic phosphorus determination in Chang and Jackson fractionation procedure. Soil Science 110, 361–362.
Casterline J L and Yip G 1975 The distribution and binding of cadmium in oyster, soybean and rat liver and kidney. Arch. Environ. Contam. Toxicol. 3, 319–329.
Cosgrove D J 1980 Inositol Phosphates: Their Chemistry, Biochemistry and Physiology. Elsevier Scientific Publishing, Amsterdam, Oxford, New York. 191 p.
DeVos C H R, Schat H, Vooijs R and Ernst W H O 1977 Copper-induced damage to the permeability barrier in roots of Silene cucubalis. J. Plant Physiol. 135, 164–169.
Dixon K W, Kuo J and Pate J S 1983 Storage reservoir of the seed-like aestivating organs of geophytes inhabiting granite outcrops in Southwestern Australia. Aust. J. Bot. 31, 85–103.
Enrst W H O 1975 Physiology of heavy metal resistance in plants. In Proc. Int. Conf. on Heavy Metals in the Environment. Eds. T C Huchinson, S Epstein, A L Page, J Van Loon and T Davey. pp 121–136. CEP Consultants, Toronto, Canada.
Franceschi V R and Schueren A M 1989 Incorporation of strontium into plant calcium oxalate crystals. Protoplasma 130, 199–205.
Franceschi V R 1989 Calcium oxalate formation is a rapid and reversible process in Lemna minor L. Protoplasma 148, 130–137.
Godbold D L, Horst W J, Collins J C, Thurman D A and Marschner H 1984 Accumulation of zinc and organic acids in roots of zinc tolerant and nontolerant ecotypes of Deschampsia caespitosa. J. Plant Physiol. 116, 59–69.
Grill E, Winnacker E-L and Zenk M H 1985 Phytochelatins: The principal heavy-metal complexing peptides of higher plants. Science 230, 674–676.
Grill E, Winnacker E-L and Zenk M H 1987 Phytochelatins, a class of heavy-metal binding peptides from plants, are functionally analogous to metallothioneins. Proc. Nat. Acad. Sci. 84, 439–443.
Hirt H, Sommergruber K and Barta A 1990 Effects of cadmium on tobacco: Synthesis and regulation of cadmium-binding peptides. Biochem. Physiol. Pflanzen 186, 153–163.
Huang B, Hatch E and Goldbrough P B 1987 Selection and characterization of cadmium tolerant cells in tomato. Plant Science 52, 211–221.
Jackson P J, Unkefer C J, Doolen J A, Watt K and Robinson N J 1987 Poly (γ-glutamyl cysteinyl) glycine: Its role in cadmium resistance in plant cells. Proc. Nat. Acad. Sci. 84, 6619–6623.
Krotz R M, Evangelou B P and Wagner G J 1989 Relation-ships between cadmium, zinc, Cd-peptide and organic acid in tobacco suspension cells. Plant Physiol. 91, 780–787.
Marschner H 1986 Mineral Nutrition of Higher Plants. Academic Press, London. 674 p.
Matthys W 1977 The role of malate, oxalate and mustard oil glycosides in the evolution of zinc-resistance in herbage plants. Physiol. Plant. 33, 161–165.
Michael G 1939 Phosphate fractions in oat grains and spinach related to a varied application of phosphorus. Bodenkd. Pflanzenernaehr. 14, 148–171.
Organ M G, Greenwood J S and Bewley J D 1988 Phytin is synthesized in the cotyledons of germinated castor-bean seeds in response to exogenously supplied phosphate. Planta 174, 513–517.
Rauser W E 1984 Isolation and partial purification of cadmium-binding protein from roots of the grass Agrostis gigantea. Plant Physiol. 74, 1025–1029.
Reddy G N and Prasad M N V 1990 Heavy metal-binding-proteins/peptides: Occurrence, structure, synthesis and functions — A review. Environ. Expt. Bot. 30, 251–264.
Reese R N and Wagner G J 1987 Effects of buthionine sulfoximine on Cd-binding peptide levels in suspension-cultured tobacco cells treated with Cd, Zn or Cu. Plant Physiol. 84, 574–577.
Reuter D R and Robinson 1986 Plant Analysis: An Interpretation Manual. Inkata Press, Melbourne, Sydney. 218 p.
Roberts R M and Loewus F 1968 Inositol metabolism in plants. VI. Conversion of myo-inositol to phytic acid in Wolffiella floridana. Plant Physiol. 43, 1710–1716.
Robinson N J and Jackson P J 1986 ‘Metallothionein-like’ metal complexes in angiosperms: Their structure and function. Physiol. Plant. 67, 499–506.
Samotus B and Schwimmer S 1962 Phytic acid as a phosphorus reservoir in the developing potato tuber. Nature 194, 578–579.
Schultz C L and Hutchinson T C 1988 Evidence against a key role for methallothionein-like protein in the copper tolerance mechanism of Deschampsia caespitosa (L.). Beauv. New Phytol. 110, 163–171.
VanBalen E, Van deGeijn S C and Desomet G M 1980 Autoradiographic evidence for the incorporation of cadmium into calcium oxalate crystals. Z. Pflanzenphysiol. 97, 123–133.
VanSteveninck R F M, VanSteveninck M E, Fernando D R, Horst W J and Marschner H 1987 Deposition of zinc phytate in globular bodies in roots of Deschampsia caespitosa ecotypes: A detoxification mechanism? J. Plant Physiol. 131, 247–257.
VanSteveninck R F M, VanSteveninck M E, Wells A J and Fernando D R 1990 Zinc tolerance and the binding of zinc as zinc phytate in Lemna minor. X-ray microanalytical evidence. J. Plant' Physiol. 137, 140–146.
VanSteveninck R F M and VanSteveninck M E 1991 Microanalysis. In Electron Microscopy of Plant Cells. Eds. J L Hall and C Hawes. pp 415–455. Academic Press, London.
Verkley J A C and Schat H 1990 Mechanisms of metal tolerance in higher plants. In Evolutionary Aspects of Heavy-Metal Tolerance in Plants. Ed. J Shaw. pp 179–193. CRC Press Boca Raton, FL.
Verkley J A C, Koevoets P, Van't Riet J, Bank R, Nydam Y and Ernst W H O 1990 Poly (g-glutamyl cysteinyl) glycines or phytochelatins and their role in cadmium tolerance of Silene vulgaris. Plant, Cell Environ. 13, 913–921.
Verkley J A C, Lolkema P C, DeNeeling A L and Harmens H 1991 Heavy-metal resistance in higher plants: Biochemical and genetic aspects. In Ecological Responses to Environmental Stresses. Eds. J Rozema and J A C Verkley. pp 8–19. Kluwer Acadmeic Publishers, Dordrecht, The Netherlands.
Wainwright S J and Woolhouse H W 1977 Some physiological effects of copper and zinc tolerance in Agrostis tenuis Sibth.: Cell elongation and membrane damage. J. Exp. Bot. 28, 1029–1036.
Wagner G J 1984 Characterization of cadmium binding complex of cabbage leaves. Plant Physiol. 76, 797–805.
Wagner G J and Krotz R M 1989 Perspectives on Cd and Zn accumulations, accommodation and tolerance in plant cells: The role of Cd-binding peptides versus other mechanisms. UCLA Symp. Mol. Cell Biol. (New Series) 98, 325–336.
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Van Steveninck, R.F.M., Van Steveninck, M.E. & Fernando, D.R. Heavy-metal (Zn, Cd) tolerance in selected clones of duck weed (Lemna minor). Plant Soil 146, 271–280 (1992). https://doi.org/10.1007/BF00012021
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DOI: https://doi.org/10.1007/BF00012021