Skip to main content
Log in

Screening maize inbred lines for tolerance to low-P stress condition

  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Genotype screening and selection for tolerance to low-phosphorus stress conditions is an important strategy for the development of cultivars growing on soils low in available P. This study was conducted to adjust an existing screening methodology which provides stable, diffusion-limited low-P concentrations for use with maize, and then use the modified methodology to select maize inbreds tolerant to low-P stress during the vegetative stage of development. Low and high-P concentration levels were established that provided a reproducible and diffusion-controlled availability of P to the plants at concentrations of 8–10 μM and 40–50 μM, respectively, of culture medium solution P at the time of transplanting. The procedure was also effective in providing low concentrations of P during the period of plant growth. The sand-alumina culture medium was used to screen 20 maize inbreds known to be efficient in P uptake or accumulation and/or tolerant to aluminum toxicity. The inbreds B37, Oh40B, NY821, Pa36, and MS1334 were selected as tolerant to low-P and WH, H99, H84, Pa32, and W37A were selected as intolerant to low-P in sand-alumina medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baker D E, Jarrel A E, Marshall L E and Thomas W I 1970 Phosphorus uptake from soils by corn hybrids selected for high and low phosphorus accumulation. Agron. J. 62, 103–106.

    Google Scholar 

  • Barber S A 1980 Soil-plant interactions in the phosphorus nutrition of plants. In The Role of Phosphorus in Agriculture. Eds. F E Khasawneh and E C Sample. Soil Sci. Soc. Am. Madison, WI.

    Google Scholar 

  • Bertramson B R 1942. Phosphorus analysis of plant material. Plant Physiol. 17, 447–455.

    Google Scholar 

  • Boawn L C and Brown J C 1968 Further evidence for P-Zn imbalance in plants. Soil Sci. Soc. Am. Proc. 32, 94–97.

    Google Scholar 

  • Brown J C 1972 Competition between phosphate and the plant for Fe from Fe2+ ferrozine. Agron. J. 64, 240–243.

    Google Scholar 

  • Burleson C A and Page N R 1967 Phosphorus and zinc interactions in flax. Soil Sci. Soc. Am. Proc. 31, 510–513.

    Google Scholar 

  • Buso G S C 1986 Variability among lettuce grown under diffusion limited conditions of phosphorus supply. M.Sc. Thesis, Univ. of Wisconsin, Madison, WI.

    Google Scholar 

  • Chaney R L and Coulombe B A 1982 Effect of phosphate on regulation of Fe-stress response in soybean and peanut. J. Plant Nutr. 5, 469–487.

    Google Scholar 

  • Clark R B 1982 Nutrient solution growth of sorghum and corn in mineral nutrition studies. J. Plant Nutr. 5, 1039–1057.

    Google Scholar 

  • Clark R B 1983 Plant genotype differences in the uptake, translocation, accumulation, and use of mineral elements required for plant growth. Plant and Soil 72, 175–196.

    Google Scholar 

  • Clark R B and Brown J C 1974 Differential phosphorus uptake by phosphorus-stressed corn inbreds. Crop Sci. 14, 505–508.

    Google Scholar 

  • Coltman R R 1983 Intraspecific variation in tomato for dry matter accumulation under maintained and diffusion-controlled phosphorus deficiency. Ph.D. Thesis, University of Wisconsin, Madison, WI.

    Google Scholar 

  • Coltman R R, Gerloff G C and Gabelman W H 1982 A sand culture system for simulating plant responses to phosphorus in soil. J. Amer. Soc. Hort. Sci. 107, 938–942.

    Google Scholar 

  • Elliott G C and Lauchli A 1985 Phosphorus efficiency and phosphate-iron interaction in maize. Agron. J. 77, 399–403.

    Google Scholar 

  • Elliott G C, Carlson R M, Lauchli A and Rosen C J 1983. A solid-phase buffer technique to maintain low concentrations of phosphate in nutrient solutions. J. Plant Nutr. 6, 1043–1058.

    Google Scholar 

  • Gerloff G C 1987 Intact-plant screening for tolerance of nutrient-deficiency, stress. In Genetic Aspects of Plant Mineral Nutrition. Eds. W H Gabelman and B C Loughman. pp 55–68. Martinus Nijhoff Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Gorsline G W, Thomas W I and Baker D E 1964 Inheritance of P, K, Mg, Cu, B, Zn, Al and Fe concentrations by corn (Zea mays L.) leaves and grain. Crop. Sci. 4, 207–210.

    Google Scholar 

  • Hoagland D R and Arnon D I 1938. The Water-culture Method for Growing Plants without Soil. Univ. California, Agric. Exp. Station, Berkeley, CA. Circular 347.

    Google Scholar 

  • Jackson M L 1958 Soil Chemical Analysis. Prentice Hall Inc., Englewood Cliffs, NJ.

    Google Scholar 

  • Magnavaca R 1982 Genetic variability and the inheritance of aluminum tolerance in maize (Zea mays L.). Ph.D. Thesis, University of Nebraska, Lincoln, NE. 135 p.

    Google Scholar 

  • Marschner H 1978 Role of the rhizosphere in iron nutrition of plants. Iran. J. Agric. Res. 6, 69–80.

    Google Scholar 

  • Marschner H 1986 Mineral Nutrition of Higher Plants. Academic Press, London. 674 p.

    Google Scholar 

  • Millikan C R 1963 Effects of different levels of zinc and phosphorus on the growth of subterranean clover (Trifolium subterraneum L.). Austr. J. Aric. Res. 14, 180–205.

    Google Scholar 

  • Murphy J and Riley J P 1962 A modified single solution method for the determination of phosphate in natural waters. Anal. Chem. Acta 27, 31–36.

    Google Scholar 

  • Nielsen N E and Barber S A 1978 Differences among genotypes of corn in the kinetics of P uptake. Agron. J. 70, 695–698.

    Google Scholar 

  • Pereira P A A 1987 Improvement of N2 fixation in common bean (Phaseolus vulgaris L.) at different levels of available phosphorus. Ph.D. Thesis, University of Wisconsin, Madison, WI.

    Google Scholar 

  • Rhue R D and Grogan C O 1977 Screening corn for Al tolerance using different Ca and Mg concentrations. Agron. J. 69, 755–760.

    Google Scholar 

  • Silva A E 1990 Inheritance studies of phosphorus acquisition and utilization efficiency in maize (Zea mays L.). Ph.D. Thesis, University of Wisconsin, Madison, WI. 149 p.

    Google Scholar 

  • Tisdale S L, Nelson W L and Beaton J D 1985 Soil Fertility and Fertilizers. Macmillan, New York. 754 p.

    Google Scholar 

  • White R E 1980 Retention and release of phosphate by soil and soil constituents. In Soil and Agriculture: Critical Reports on Applied Chemistry. Vol. 2. Ed. P B Tinker. pp. 71–114. Blackwell Scientific Publications, Oxford.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Da Silva, Á.E., Gabelman, W.H. Screening maize inbred lines for tolerance to low-P stress condition. Plant Soil 146, 181–187 (1992). https://doi.org/10.1007/BF00012011

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00012011

Key words

Navigation