Abstract
In the present study, the effect of Alnus glutinosa seed extract on the in vitro growth of Frankia strain ArI3 was investigated under various culture conditions. Frankia filaments grown in static cultures showed a very slow growth with doubling time of 12 days. However, under the same growth conditions but with seed extract added to the medium, the doubling time was reduced to 6 days. A further reduction in doubling time (2.5 days) was obtained without seed extract when Frankia strain ArI3 was grown in a fermentor with continuous stirring. Under these culture conditions, seed extract did not influence the Frankia growth rate. In all our studies, Na-propionate was used as the only carbon source. Gradual addition of Na-propionate to Frankia cultures in the fermentor sustained exponential growth of the filaments over a prolonged period. The complete consumption of Na-propionate was the limiting growth factor under fermentor growth conditions. The mechanism of growth stimulation by Alnus glutinosa seed extract under growth limiting conditions in static cultures and its possible ecological significance is discussed.
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Baker D and Mullin B C 1992 Actinorhizal symbioses. In Biological Nitrogen Fixation. Eds. GStacey, R HBurris and H JEvans. pp 257–292. Chapman and Hall, New York.
Berry A and Torrey J 1979 Isolation and characterization in vivo and in vitro of an actinomycetous endophyte from Alnus rubra Bong. In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Weeler and P A Perry. pp 69–83. Forest Research Laboratory. Oregon State University.
Blom J 1981 Utilization of fatty acids and NH4 + by Frankia AvcIl. FEMS Microbiol. Lett. 10, 143–145.
Blom J 1982 Carbon and nitrogen source requirements in Frankia strains. FEMS Microbiol. Lett. 13, 51–55.
Blom J and Harkink R 1981 Metabolic pathways for gluconeogenesis and energy generation in Frankia AvcIl. FEMS Microbiol. Lett. 11, 221–224.
Blom J, Roelofsen W and Akkermans A D L 1980 Growth of Frankia AvcIl on media containing Tween 80 as C-source. FEMS Microbiol. Lett. 9, 131–135.
Burggraaf A J P and Shipton W A 1983 Studies on the growth of Frankia isolates in relation to infectivity and nitrogen fxation (acetylene reduction). Can. J. Bot. 61, 2774–2782.
Girgis M G Z and Schwencke J 1993 Differentiation of Frankia strains by their electrophoretic patterns of intracellular esterases and aminopeptidases. J. Gen. Microbiol. 139, 2225–2232.
Hartwig U A, Joseph C M and Phillips D A 1991 Flavonoids released naturally from alfalfa seeds enhance growth rate of Rhizobium meliloti. Plant Physiol. 95, 797–803.
Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951 Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.
Meesters T M, van Genesen S T and Akkermans A D L 1985 Growth, acetylene reduction activity and localization of nitrogenase in relation to vesicle formation in Frankia strain Ccl. 17 and Cpl. 2. Arch. Microbiol. 143, 137–142.
Murry M A, Fontaine M S and Torrey J G 1984 Growth kinetics and nitrogenase induction in Frankia sp. HFP ArI3 grown in batch culture. Plant and Soil 78, 61–78.
Perradin Y, Mottet M J and Lalonde M 1983 Influence of phenolics on in vitro growth of Frankia strains. Can. J. Bot. 61, 2807–2814,
Phillips D A, Dakora F D, Leon-Barris M, Sande E and Joseph C M 1993 Signals released from alfalfa regulate microbial activity in the rhizosphere. In Horizons in Nitrogen Fixation. Eds. RPalacios, JMora and W ENewton. pp 197–202. Kluwer Academic Publishers. Dordrecht.
Quispel A, Burggraaf A J P, Borsje H and Tak T 1983 The role of lipids in the growth of Frankia isolates. Can. J. Bot. 61, 2801–2807.
Quispel A, Svendsen A B, Schripsema J, Baas W J, Erkelenes C and Lugtenberg J 1989 Identification of dipterocarpol as isolation factor for the induction of primary isolation of Frankia from root nodules of Alnus glutinosa (L.) Gaertner. Mol. Plant-Microbe Interact. 2, 107–112.
Schwencke J 1991 Rapid, exponential growth and increased biomass yield of some Frankia strains in buffered and stirred mineral medium (BAP) with phosphatidyl choline. Plant and Soil 137, 37–41.
Steele B B, Ramirez K and Stowers M D 1989 Host plant growth response to inoculation with Frankia. Plant and Soil 118, 139–143.
Tisa L, McBride M and Ensign J C 1983 Studies of growth and morphology of Frankia strain EANlpec, EuIlc, CpIl, and ACNlAG Can. J. Bot. 61, 2768–2773.
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Ringø, E., Clausen, E., Løvaas, E. et al. Effects of extracts of Alnus glutinosa seeds on growth of Frankia strain ArI3 under static and fermentor culture conditions. Plant Soil 176, 283–288 (1995). https://doi.org/10.1007/BF00011792
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DOI: https://doi.org/10.1007/BF00011792