Experimental and genetic analysis of root development inArabidopsis thaliana

Abstract

The cellular organisation of theArabidopsis thaliana root is remarkably regular. A fate map of the primary root and root meristem that predicts the developmental destinies of cells within the embryonic root primordium has been constructed. Nevertheless, laser ablation experiments demonstrate that root meristem cells develop according to position and not according to lineage. Mutational analysis has identified genes required for cell specification in the radial as well as in the apical-basal dimension. The corresponding gene functions appear to be necessary during embryogenesis for the formation of a correctly patterned primary root. H Lambers Section editor

This is a preview of subscription content, access via your institution.

References

  1. Van denBerg C, Willemsen V, Hage W, Weisbeek P and Scheres B 1955 Cell fate in theArabidopsis root meristem determined by directional signalling. Nature 378, 62–65.

    Google Scholar 

  2. Berger F, Taylor A and Brownlee C 1994 Cell fate determination by the cell wall in earlyFucus development. Science 263, 1421–1423.

    Google Scholar 

  3. Berleth T and Jürgens G 1993 The role of the monopteros gene in organising the basal body region of theArabidopsis embryo. Development 118, 575–587.

    Google Scholar 

  4. Cheng J-C, Seeley K A and Sung Z R 1995 RML1 and RML2,Arabidopsis genes required for cell proliferation at the root tip. Plant Physiol. 107, 365–376.

    Google Scholar 

  5. Clowes F A L 1961 Apical Meristems. Blackwell, Oxford, UK.

    Google Scholar 

  6. Dolan L, Janmaat K, Willemsen V, Linstead P, Poethig S, Roberts K and Scheres B 1993 Cellular organisation of theArabidopsis root. Development 119, 71–84.

    Google Scholar 

  7. Dolan L, Duckett C M, Grierson C, Linstead P, Schneider K, Lawson E, Dean C, Poethig S and Roberts K 1994 Clonal relationships and cell patterning in the root epidermis ofArabidopsis. Development 120, 2465–2474.

    Google Scholar 

  8. Duckett C M, Oparka K J, Prio D A M, Dolan L and Roberts K 1994 Dye-coupling in the root epidermis ofArabidopsis is progressively reduced during development. Development 120, 3247–3255.

    Google Scholar 

  9. Guttenberg H v, Burmeister J and Brosell H-J 1955 Studien uber die Entwicklung des Wurzelvegetationspunktes der Dikotyledonen II. Planta 46, 179–222.

    Google Scholar 

  10. Hanstein J 1870 Die Entwicklung des Keimes der Monocotyledon und der Dikotylen. Bot. Abhandl. (Bonn) 1–112.

  11. Hejnowicz and Hejnowicz K 1991 Modeling the formation of root apices. Planta 184, 1–7.

    Google Scholar 

  12. Jürgens G 1995 Axis formation in plant embryogenesis: cues and clues. Cell 81, 467–470.

    Google Scholar 

  13. Kaplan D R and Hagemann W 1991 The relationship of cell and organism in vascular plants. Bioscience 41, 693–703.

    Google Scholar 

  14. Poethig R S 1987 Clonal analysis of cell lineage patterns in plant development. Am. J. Bot. 74, 581–594.

    Google Scholar 

  15. Scheres B, Wolkenfelt H, Willemsen V, Terlouw M, Lawson E, Dean and Weisbeek P 1994 Embryonic origin of theArabidopsis primary root and root meristem initials. Development 120, 2475–2487.

    Google Scholar 

  16. Scheres B, Di Laurenzio L, Willemsen V, Hauser M-T, Janmaat K, Weisbeek P and Benfey P N 1995 Mutations affecting the radial organisation of theArabidopsis root display specific defects throughout the embryonic axis. Development 121, 53–62.

    Google Scholar 

  17. Steeves T A and Sussex I M 1989 Patterns in plant development. Cambridge University Press, Cambridge, UK.

    Google Scholar 

  18. Torres-Ruiz R A and Jurgens G 1994 Mutations in the FASS gene uncouple pattern formation and morphogenesis inArabidopsis development. Development 120, 2967–2978.

    Google Scholar 

  19. Weigel D and Meyerowitz E M 1994 The ABCs of floral homeotic genes. Cell 78, 203–209.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Scheres, B., McKhann, H., van den Berg, C. et al. Experimental and genetic analysis of root development inArabidopsis thaliana . Plant Soil 187, 97–105 (1996). https://doi.org/10.1007/BF00011661

Download citation

Key words

  • Arabidopsis
  • cell fate
  • embryogenesis
  • laser ablation
  • root meristem