Abstract
A Cd-tolerant arbuscular mycorrhizal fungus, Glomus mosseae, isolated from a polluted soil (P2 culture), was compared with a Cd-sensitive reference Glomus mosseae (Gm) for its capacity to colonize maize (Zea mays L.) roots and to affect plant growth and Cd uptake in sand culture at increasing exposure to Cd added with the nutrient solution (0, 0.1, 1, 5 and 10 mg L-1). After eight weeks, mycorrhizal colonization by P2 culture was relatively high (50% of the control without Cd added) up to 5 mg L-1 Cd, whereas colonization by Gm was almost totally suppressed at that Cd level. However, even mycorrhizal colonization by the Cd-tolerant P2 culture appeared more sensitive to Cd than plant growth and was completely suppressed at 10 mg L-1 Cd. AM colonization did not alleviate the negative effect of Cd on plant growth compared to the non-mycorrhizal treatment. On the contrary, at the 5 mg L-1 Cd level non-mycorrhizal plants were greater than mycorrhizal plants with lower Cd concentration.
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References
Babich H and Stotzky G 1978 Effects of cadmium on the biota: Influence of environmental factors. Adv. Appl. Microbiol. 23, 55–117.
Bethlenfalvay G J, Brown M S and Pakovsky R S 1982 Parasitic and mutualistic associations between a mycorrhizal fungus and soybean: Development of the host plant. Phytopathol. 72, 889–893.
Buwalda J G and Goh K M 1982 Host-fungus competition for carbon as a cause of growth depressions in vesicular-arbuscular mycorrhizal ryegrass. Soil Biol. Biochem. 14, 103–106.
Chao C-C and Wang Y-P 1990 Effects of heavy metals on the infection of vesicular-arbuscular mycorrhizae and the growth of maize. Jo. Agric. Assoc. China 152, 34–45.
Chao C-C and Wang Y-P 1991 Effects of heavy metals on vesicular-arbuscular mycorrhizae and nitrogen fixation of soybean in major soil groups of Taiwan. Jo. Chin. Agric. Chem. Soc. 29, 290–300.
Clapperton M J and Reid D M 1992 A relationship between plant growth and increasing VA mycorrhizal inoculum density. New Phytol. 120, 227–234.
Cooper K M and Tinker P B 1978 Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. II. Uptake and translocation of phosphorus, zinc and sulphur. New Phytol. 81, 43–52.
El-Kherbawy M, Angle J S, Heggo A and Chaney R L 1989 Soil pH, rhizobia, and vesicular-arbuscular mycorrhizae inoculation effects on growth and heavy metal uptake of alfalfa (Medicago sativa L.). Biol. Fertil. Soils 8, 61–65.
Foy C D, Chaney R L and White M C 1978 The physiology of metal toxicity in plants. Ann. Rev. Plant Physiol. 29, 511–566.
Gildon A and Tinker P B 1983 Interactions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. I. The effect of heavy metals on the development of vesicular arbuscular mycorrhizas. New Phytol. 95, 247–261.
Hewitt E J 1966 Sand and water culture methods used in the study of plant nutrition. Technical Communications. No 22, 2nd ed. revised. Commonwealth Agricultural Bureau, London.
Jackson A P and Alloway B J 1992 The transfer of cadmium from agricultural soils to the human food chain. In Biogoechemistry of Trace Metals. Advances in Trace Sustances Research. Ed. D C Adriano. pp 109–158. Lewis Publishers, Boca Raton, FL.
Klein H, Priebe A and Jäger H-J 1981 Grenzen der Belastbarkeit von Kulturpflanzen mit dem Schwermetall Cadmium. Angew. Bot. 55, 295–308.
Kothari S K, Marschner H and Römheld V 1991 Contribution of the VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcerous soil. Plant and Soil 131, 177–185.
Leyval C, Berthelin J, Schontz D, Weissenhorn I and Morel J-L 1991 Influence of endomycorrhizas on maize uptake of Pb, Cu, Zn and Cd applied as mineral salts or sewage sludge. In Heavy Metals in the Environment. Ed. J G Farmer. pp 204–207 CEP Consultants Ltd, Edinburgh.
Li X-L, Marschner H and Römheld V 1991 Acquisition of phosphorus and copper by VA-mycorrhizal hyphae and root-to-shoot transport in white clover. Plant and Soil 136, 49–57.
Manjunath A and Habte M 1988 Development of vesicular-arbuscular mycorrhizal infection and the uptake of immobile nutrients in Leucaena leucocephala. Plant and Soil 106, 97–103.
Pearson J N and Jakobsen I 1993 The relative contribution of hyphae and roots to phosphorus uptake by arbuscular mycorrhizal plants, measured by dual labelling with P-32 and P-33. New Phytol. 124, 489–494.
Sauerbeck D 1982 Welche Schwermetallgehalte in Pflanzen dürfen nicht überschritten werden, um Wachstumsbeeinträchtigungen zu vermeiden? Landwirtsch. Forsch., Sonderheft 39, 105–129.
Schüepp H, Dehn B and Sticher H 1987 Interaktionen zwischen VA-Mykorrhizen und Schwermetallbelastungen. In Mycorrhiza and Plant Stress. Angew. Bot. 61, 85–95.
Tisserant B, Gianinazzi-Pearson V, Gianinazzi S and Gollotte A 1993 In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections. Mycol. Res. 97, 245–250.
Trouvelot A, Kough J L and Gianinazzi-Pearson V 1986 Mesure du taux de mycorhization VA d'un système radiculaire. Recherche de méthodes d'estimation ayant une signification fonctionelle. In Physiological and Genetical Aspects of Mycorrhizae. Eds. V Gianinazzi-Pearson and S Gianinazzi. pp 217–221. INRA Press, Paris.
Weissenhorn I, Leyval C and Berthelin J 1993 Cd-tolerant arbuscular mycorrhizal (AM) fungi from heavy-metal polluted soils. Plant and Soil 157, 247–256.
Weissenhorn I, Leyval C and Berthelin J 1995a Bioavailability of heavy metals and abundance of arbuscular mycorrhiza in a soil polluted by atmospheric deposition from a smelter. Biol. Fertil. Soils 19, 22–28.
Weissenhorn I, Mench M and Leyval C 1995b Bioavailability of heavy metals and arbuscular mycorrhiza in a sewage-sludge amended soil. Soil Biol. Biochem, 27, 287–296.
Weissenhorn I, Leyval C, Belgy G and Berthelin J 1995c Arbuscular mycorrhizal (AM) contribution to heavy metal uptake by maize (Zea mays L.) in pot culture with contaminated soil. Mycorrhiza (In press).
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Weissenhorn, I., Leyval, C. Root colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture. Plant Soil 175, 233–238 (1995). https://doi.org/10.1007/BF00011359
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DOI: https://doi.org/10.1007/BF00011359