Abstract
Two cultivars of Phaseolus vulgaris L., one responsive to colonization with microsymbionts (Mexico 309) and one less-responsive (Rio Tibagi) were grown in Leonard jars containing sand/vermiculite under greenhouse conditions. Bean plants were either left non-inoculated (controls) or were inoculated with the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus etunicatum or a strain of Rhizobium leguminosarum bv. phaseoli (UMR-1899). Plants from the Mexico 309 cultivar maintained a higher growth rate, supported proportionately more nodules and mycorrhizae, and assimilated relatively more N or P when colonized by Rhizobium or Glomus, respectively, than did plants of the Rio Tibagi cultivar. Estimated specific nodule activity for Mexico 309 beans was more than twice that of Rio Tibagi, whereas the specific phosphorus uptake rate (SPUR) was 35% greater in the non-inoculated roots of Rio Tibagi compared to Mexico 309. Colonization by G. etunicatum more than doubled the SPUR for each cultivar compared to control roots. New acid phosphatase isozymes appeared in VAM-colonized roots of both cultivars compared to controls. Acid and alkaline phosphatase activities were significantly higher in G. etunicatum-colonized Mexico 309 roots, but not in Rio Tibagi mycorrhizae, compared to uninfected roots. Polyphosphate hydrolase activity was elevated in mycorrhizae of both cultivars compared to control roots. These results indicate that the dependence of a host on a specific endophyte increases when there are limitations to the supply of a nutrient that the endophyte can provide. The greater the increase in absorption or utilization capacity following colonization by the microsymbiont, the greater the dependence by the host. More importantly, identification of enzymatic activities that influence these plant-microbe associations opens the possibility that the specific genes that code for these enzymes could be targeted for future manipulation.
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References
Barea J M and Azcon-Aguilar C 1983 Mycorrhizas and their significance in nodulating nitrogen-fixing plants. Adv. Agron. 36, 1–54.
Bethlenfalvay G J, Pacovsky R S, Bayne H G and Stafford A E 1982 Interactions between nitrogen fixation, mycorrhizal colonization, and host plant growth in the Phaseolus-Rhizobium-Glomus symbiosis. Plant Physiol. 70, 446–450.
Bieleski R L 1973 Phosphate pools, phosphate transport and phosphate ability. Annu. Rev. Plant Physiol. 24, 225–252.
Callow J A, Capaccio L C M, Parish T and Tinker P B 1978 Detection and estimation of polyphosphate in vesicular-arbuscular mycorrhizas. New Phytol. 80, 125–134.
Capaccio L C M and Callow J A 1982 The enzymes of polyphosphate metabolism in vesicular-arbuscular mycorrhizas. New Phytol. 91, 81–91.
Chapman H D and Pratt P F 1982 Methods of Analysis of Soils, Plants and Waters. Second Edn. University of California Press, Berkeley, CA. 310 p.
Daft M J and El-Giahmi A A 1978 Effect of an arbuscular mycorrhiza on plant growth VII. Effects of defoliation and light on selected hosts. New Phytol. 82, 365–372.
Davis B J 1964 Disc electrophoresis II. Method and application to human serum proteins. Ann. N Y Acad. Sci. 121, 404–427.
Duff S M G, Lefebvre D D and Plaxton W C 1989 Phosphate starvation inducible ‘bypasses’ of adenylate and phosphate dependent glycolytic enzymes in Brassica nigra suspension cells, Plant Physiol. 90, 1275–1278.
Freund R J and Littell R C 1981 SAS for linear models: A guide to the ANOVA and GLM procedures. SAS Institute, Raleigh, NC.
Gianinazzi-Pearson V and Gianinazzi S 1976 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. I. Effect of mycorrhiza formation and phosphorus nutrition on soluble phosphatase activities in onion roots. Physiol. Veg. 14, 833–841.
Gianinazzi-Pearson V and Gianinazzi S 1978 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. II. Soluble alkaline phosphatase specific to mycorrhizal infection in onion roots. Physiol. Plant Pathol. 12, 45–53.
Goldstein A H, Baertlein D A and McDaniel R G 1988 Phosphate starvation inducible metabolism in Lycopersicon esculentum. I. Excretion of acid phosphatase by tomato plants and suspension cultured cells. Plant Physiol. 87, 711–715.
Graham P H and Temple S R 1984 Selection for improved nitrogen fixation in Glycine max (L.) Merr. and Phaseolus vulgaris L. Plant and Soil 82, 315–327.
Graham P H 1981 Some problems of nodulation and symbiotic nitogen fixation in Phaseolus vulgaris. A Review. Field Crops Res. 4, 93–112.
Graham P H and Rosas J C 1977 Growth and development of intermediate bush and climbing cultivars of Phaseolus vulgaris L. inoculated with Rhizobium. J. Agric. Sci. (Cambridge) 88, 503–508.
Hohenberg J S, Munns D N and Tucker C L 1982 Rhizobium host specificities in Phaseolus coccineus L. and Phaseolus vulgaris L. Crop Sci. 22, 455–459.
Hungria M and Neves M C P 1986 Ontogenia da fixacão biológica do nitrogenio em Phaseolus vulgaris. Pesq. agropec. bras. 21, 715–730.
Iruthayathas E E and Vlassak K 1982 Symbiotic specificity in nodulation and nitrogen fixation between winged bean and Rhizobium. Scientia Horticul. 16, 313–322.
Israel D W 1981 Cultivar and Rhizobium strain effects on nitrogen fixation and remobilization by soybeans. Agron. J. 73, 509–516.
Kucey R M N 1989 Responses of field bean (Phaseolus vulgaris L.) to levels of Rhizobium leguminosarum bv. phaseoli inoculation in soils containing effective R. leguminosarum bv. phaseoli populations. Can. J. Plant Sci. 69, 419–426.
Lefebvre D D, Duff S M G, Fife C A, Julien-Inalsingh C and Plaxton W C 1990 Response to phosphorus deprivation in Brassica nigra suspension cells. Plant Physiol. 93, 504–511.
McKnight T 1949 Efficiency of isolates of Rhizobium in the cowpea group, with proposed additions to this group. Queensland J. Agric. Sci. 6, 61–76.
Minchin F R, Summerfield R J and Eaglesham A R J 1978 Plant genotype × Rhizobium strain interactions in cowpea (Vigna unguiculata L. Walp.) Tropical Agric. (Trinidad) 55, 107–115.
Pacovsky R S 1989 Carbohydrate, protein and amino acid status of Glycine-Glomus-Bradyrhizobium symbioses. Physiol. Plant. 75, 346–354.
Pacovsky R S and Fuller G 1986 Development of two endomycorrhizal symbioses on soybean and comparison with phosphorus fertilization. Plant and Soil 195, 361–377.
Pacovsky R S and Fuller G 1991 Nitrogen assimilation and partitioning in two nitrogen-fixing cultivars of Phaseolus vulgaris L. Plant and Soil 132, 139–148.
Pacovsky R S, Bayne H G and Bethlenfalvay G J 1984 Symbiotic interactions between strains of Rhizobium phaseoli and cultivars of Phaseolus vulgaris L.. Crop Sci. 24, 101–105.
Pereira P A A, Barris R H and Bliss F A 1989 15N-Determined dinitrogen fixation potential of genetically diverse bean lines (Phaseolus vulgaris L.). Plant and Soil 120, 171–179.
Rennie R J and Kemp G A 1983 N2-fixation in field beans quantified by 15N isotope dilution. II. Effect of cultivars of beans. Agron. J. 75, 645–649.
Ruschel A P, Vose P B, Matsui E, Victoria R L and Saito S M T 1982 Field evaluation of N2 fixation and N utilization by Phaseolus bean varieties cetermined by 15N isotope dilution. Plant and Soil 65, 397–407.
Sen R and Hepper C 1986 Characterization of vesicular-arbuscular mycorrhizal fungi (Glomus spp.) by selective enzyme staining following polyacrylamide gel electrophoresis. Soil Biol. Biochem. 18, 29–34.
Smith S S E 1980 Mycorrhizas of autotrophic higher plants. Biol. Rev. 55, 475–510.
Vincent J M 1970 Manual for the practical Study of Root Nodule Bacteria. International Biology Program Handbook. Blackwell Scientific Publishers. Oxford. 164 p.
Westermann D T and Kolar J J 1978 Symbiotic N2 (C2H2) fixation by bean. Crop Sci. 18, 986–990.
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Pacovsky, R.S., Da Silva, P., Carvalho, M.T. et al. Growth and nutrient allocation in Phaseolus vulgaris L. colonized with endomycorrhizae or Rhizobium. Plant Soil 132, 127–137 (1991). https://doi.org/10.1007/BF00011019
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DOI: https://doi.org/10.1007/BF00011019