Abstract
The ability of the benthic cyanobacterium Lyngbya wollei to fix nitrogen was studied using field samples and axenic cultures. L. wollei was collected and isolated from Lake Okeechobee, Florida, where it forms extensive mats. Rates of acetylene reduction up to 39.1 nmol mg dry wt−1 h−1 were observed for field samples. The maximum observed rate of acetylene reduction in axenic laboratory cultures was 200 nmol mg dry wt−1 h−1. Aerobic conditions limited nitrogen fixation activity, but dark/light cycles promoted the development of activity. Reduced oxygen levels appeared to be required for the development of significant levels of nitrogenase activity. The level of irradiance also had a significant impact on the level of activity. The potential significance of nitrogen fixation to Lyngbya production is discussed.
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References
American Public Health Association, 1985. Standard Methods. 16th Edition. American Public Health Association, Washington D.C., USA.
Ashton, P. J., 1981. Nitrogen fixation and the nitrogen budget of a eutrophic impoundment. Water Res. 15: 823–833.
Bautista, M. F. & H. W. Paerl, 1985. Diel N2 fixation in an intertidal marine cyanobacterial mat community. Mar. Chem. 16: 369–377.
Beer, S., W. Spencer & G. Bowes, 1986. Photosynthesis and growth of the filamentous blue-green alga Lyngbya wollei in Relation to its Environment. J. Aquat Plant Mgmt 4: 61–65.
Bebout, B. M., H. W. Paerl, K. M. Crocker & L. E. Prufert, 1987. Diel interactions of oxygenic photosynthesis and N2 fixation (acetylene reduction) in a marine microbial mat community. Appl. Envir. Microbiol. 55: 369–384.
Capone, D. G., 1983. Benthic nitrogen fixation. In E. Carpenter & D. Capone (eds), Nitrogen in the Marine Environment. Academic Press, New York: 105–138.
Carlton, R. G. & H. W. Paerl, 1989. Oxygen-induced changes in morphology of aggregates of Aphanizomenon flos-aquae (Cyanophyceae): implications for nitrogen fixation potentials. J. Phycol. 25: 326–333.
Horne, A. J. & A. B. Viner, 1971. Nitrogen fixation and its significance in tropical Lake George, Uganda. Nature 232: 417–418.
Griffiths, M. S. H., J. R. Gallon & A. E. Chaplin, 1987. The diurnal pattern of dinitrogen fixation by cyanobacteria in situ. New Phytol. 107: 649–657.
Hardy, R. W. F., R. D., Holsten E. K. Jacskon & R. C. Burns, 1968. The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Pl. Physiol. 43: 1185–1207.
Howarth, R. W., R. Marino, J. Lane & J. J. Cole, 1988. Nitrogen fixation in freshwater, estuarine, and marine ecosystems. 1. rates and importance. Limnol. Oceanogr. 33: 669–687.
Kallas, T., R. Rippka, T. Coursin, M. C. Rebiere, N. Tandeau de Marsac & G. Cohen-Bazire, 1983. Aerobic nitrogen fixation by non-heterocystous cyanobacteria. In G. C. Papageorgiou & L. Packer (eds), Photosynthetic Prokaryotes, Cell Differentiation and Function. Elsevier, New York, Amsterdam, Oxford: 281–302.
Khamees, H. S., J. R. Gallon & A. E. Chaplin, 1987. The pattern of acetylene reduction by cyanobacteria grown under alternating light and darkness. Br. Phycol. J. 22: 55–60.
Mullineaux, P. M., J. R. Gallon & A. E. Chaplin, 1981. Nitrogen fixation in cultures of the cyanobacterium Gloeocapsa sp. 1430/3 incubated in the dark. J. Gen. Microbiol. 124: 141–146.
Paerl, H. W., 1985. Microzone formation: its role in the enhancement of aquatic N2 fixation. Limnol. Oceanogr. 30: 1246–1252.
Paerl, H. W. & L. E. Prufert, 1987. Oxygen-poor microzones as potential sites of microbial N2 fixation in nitrogen depleted aerobic marine waters. Appl. Envir. Microbiol. 53: 1078–1087.
Paerl, H. W., B. M. Bebout & L. E. Prufert, 1989. Naturally occurring patterns of oxygenic photosynthesis and N2 fixation in a marine microbial mat: physiological and ecological ramifications. In Y. Cohen & E. Rosenberg (eds), Microbial Mats. American Society for Microbiology, Washington, D.C.: 326–341.
Pearson, H. W., G. Malin & R. Howsley, 1981. Physiological studies on in vivo nitrogenase activity by axenic cultures of the blue-green algae Microcoleus chthonoplastes. Br. Phycol. J. 16: 139.
Phlips, E. J., 1987. Nitrogen fixation in marine communities and ts potential role in seaweed cultivation. In K. T. Bird & P. H. Benson (eds), Seaweed Cultivation for Renewable Resources. pp. 224–284. Elsevier, New York: 224–284.
Phlips, E. J. & A. Mitsui, 1981. Role of light intensity and Temperature in the regulation of hydrogen photoproduction by the marine cyanobacterium Oscillatoria sp. Strain Miami BG 7. Appl. Envir. Microbiol. 45, 1212–1220.
Phlips, E. J., M. Willis & A. Verchick, 1986. Aspects of nitrogen fixation in Sargassum communities off the coast of Florida. J. Exp. mar. Biol. Ecol. 102: 99–119.
Potts, M. & B. A. Whitton, 1977. Nitrogen fixation by blue-green algal communities in intertidal zone of the Lagoon of Aldabra Atoll. Oecologia 27: 275–283.
Rippka, R. & R. Y. Stanier, 1978. The effects of anaerobiosis on nitrogenase synthesis and heterocyst development by nostocacean cyanobacteria. J. gen. Microbiol. 105: 83–94.
Rippka, R. & J. B. Waterbury, 1977. The synthesis of nitrogenase by non-heterocystous cyanobacteria. FEMS Microbiol. Letters 2: 83–86.
Saino, T. & A. Hattori, 1982. Aerobic nitrogen fixation by the marine non-heterocystous cyanobacterium Trichodesmium (Oscillatoria) spp.: Its protective mechanism against oxygen. Mar. Biol. 70: 251–254.
Stal, L. J. & W. E. Krumbein, 1985. Nitrogenase activity in the non-heterocystous cyanobacterium Oscillatoria sp. grown under alternating light-dark cycles. Arch. Microbiol. 143: 67–71.
Stal, L. J. & W. E. Krumbein, 1987. Temporal separation of nitrogen fixation and photosynthesis in the filamentous, non-heterocystous cyanobacterium Oscillatoria sp. Arch. Microbiol. 143: 67–71.
Stal, L. J., H. Heyer, S. Bekker, M. Villbrandt & W. E. Krumbien, 1989. Aerobic-anaerobic metabolism in the cyanobacterium Oscillatoria limosa. In Y. Cohen & E. Rosenberg (eds), Microbial Mats. American Society for Microbiology, Washington, D.C.: 255–276.
Taylor, B., 1983. Assays of microbial nitrogen transformations. In E. J. Carpenter & D. G. Capone (eds), Nitrogen in the Marine Environment. Academic Press, New York: 809–838.
Wetzel, R. G., 1983. Limnology. 2nd Edition. Saunders College Publ., Philadelphia, PA.
Whitney, D. E., G. M. Woodwell & R. W. Howarth, 1975. Nitrogen fixation in a Flax Pond: a Long Island salt marsh. Limnol. Oceanogr. 20: 640–643.
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Phlips, E.J., Ihnat, J. & Conroy, M. Nitrogen fixation by the benthic freshwater cyanobacterium Lyngbya wollei . Hydrobiologia 234, 59–64 (1992). https://doi.org/10.1007/BF00010779
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DOI: https://doi.org/10.1007/BF00010779