Abstract
Seedlings of pine, infected with two different ectomycorrhizal fungi, Suillus bovinus (Fr.) O. Kuntze and an unidentified isolate (BP), were exposed to various external Zn concentrations. An additional strain of S. bovinus, cultured on a Zn-enriched medium, was also used. The effect of mycorrhizal associations on Zn uptake and distribution within the plant was determined by AAS.
The results show that nonmycorrhizal seedlings have the capability to control the uptake and translocation of Zn to the shoot until the external Zn concentration reaches a threshold level, where no limitation of uptake is possible. Excess Zn is accumulated in the root system to protect the shoot against toxic tissue concentrations. The effect of an ectomycorrhizal infection on Zn uptake and distribution depends on (1) the fungal species (2) the external concentration and (3) the Zn content of the fungal culture medium. Under conditions of low external Zn supply, especially a mycorrhizal infection with S. bovinus led to an increased Zn uptake in root and needles of Pinus sylvestris. Under high external conditions the mycobionts varied considerably in their capability to reduce the transport of Zn to the shoot. Only by an infection with S. bovinus the plant was able to maintain the shoot tissue concentration on a low level. This effect can be enhanced by pretreatment of S. bovinus with high Zn concentrations.
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References
Bergmann W 1983 Ernährungsstörungen bei Kulturpflanzen. Gustav Fischer Verlag Stuttgart.
Bowen G D, Skinner M F and Bevege D I 1974 Zinc uptake by mycorrhizal and uninfected roots of Pinus radiata and Araucaria cunninghamii. Soil Biol. Biochem. 6, 141–144.
Bradley R, Burt A J and Read D J 1982 Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris. Nature 292, 335–337.
Bradley R, Burt A J and Read D J 1982 The biology of mycorrhiza in the Ericaceae. VIII. The role of mycorrhizal infection in heavy metal resistance. New Phytol. 91, 197–201.
Brown M T and Wilkins D A 1985a Zinc tolerance of Amanita and Paxillus. Trans. Br. Mycol. Soc. 84(2), 367–369.
Brown M T and Wilkins D A 1985b Zinc tolerance of mycorrhizal Betula. New Phytol. 99, 101–106.
Colpaert J V and VanAssche J A 1992 Zinc toxicity in ectomycorrhizal Pinus sylvestris. Plant and Soil 143, 201–211.
DeKock P C and Mitchell R L 1957 Uptake of chelated metals by plants. Soil Sci. 84, 55–62.
Denny H J and Wilkins D A 1987a Zinc tolerance in Betula spp. I. Effect of external concentration of zinc on growth and uptake. New Phytol. 106, 517–524.
Denny H J and Wilkins D A 1987b Zinc tolerance in Betula spp. II. Microanalytical studies of zinc uptake into root tissues. New Phytol. 106, 535–534.
Denny H J and Wilkins D A 1987c Zinc tolerance in Betula spp. III. Variation in response to zinc among ectomycorrhizal associates. New Phytol. 106, 535–544.
Denny H J and Wilkins D A 1987d Zinc tolerance in Betula spp. IV. The mechanism of ectomycorrhizal amelioration of zinc toxicity. New Phytol. 106, 545–553.
Gildon A and Tinker P B 1983 Interactions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. I. The effects of heavy metals on the development of vesicular-arbuscular mycorrhizas. New Phytol. 95, 247–261.
Harley J L and Smith S E 1983 Mycorrhizal Symbiosis. Academic Press, London.
Heinrichs H, Brumsack H-J, Loftfield N and König N 1986 Verbessertes Druckaufschlusssystem fur biologische und anorganische Materialien. Z. Pflanzenernaehr. Bodenkd. 149, 350–353.
Hilger A B, Thomas K I and Krause H H 1986 The effect of several buffers on growth and phosphorus nutrition of selected ectomycorrhizal fungi. Soil Biol. Biochem. 18, 61–67.
Ingestad T 1960 Studies on the nutrition of forest tree seedlings. III. Mineral nutrition of pine. Physiol. Plant. 13, 513–533.
Jarvis S C, Jones L H P and Hopper M J 1976 Cadmium uptake from solution by plants and its transport from roots to shoots. Plant and Soil 44, 179–191.
Jones M D, Dainty J and Hutchinson T C 1988 The effect of infection by Lactarius rufus or Scleroderma flavidum on the uptake of 63Ni by paper birch. Can. J. Bot. 66, 934–940.
Jones M D and Hutchinson T C 1988a Nickel toxicity in mycorrhizal birch seedlings infected with Lactarius rufus or Scleroderma flavidum. I. Effects on growth, photosynthesis, respiration and transpiration. New Phytol. 108, 451–459.
Jones M D and Hutchinson T C 1988b Nickel toxicity in mycorrhizal birch seedlings infected with Lactarius rufus or Scleroderma flavidum. II. Uptake of nickel, calcium, magnesium, phosphorus and iron. New Phytol. 108, 461–470.
Kumpfer W 1991 Untersuchungen zur Bedeutung der Ektomycorrhiza für Zinkernährung und Zinktoleranz von Fagus sylvatica L. und Pinus sylvestris L., Ph.D. Thesis, University of Bremen, FRG.
Lyr H, Fiedler H-J and Tranquillini W 1992 Physiologie und Ökologie der Gehölze. Gustav Fischer, Jena. pp 71–78.
Mitchell C D and Fretz T A 1977 Cadmium and zinc toxicity in white pine, red maple, and norway spruce. J. Am. Soc. Hortic. Sci. 102(1), 81–84.
Morselt A F W, Smits W T M and Limonard T 1986. Histochemical demonstration of heavy metal tolerance in ectomycorrhizal fungi. Plant and Soil 96, 417–420.
Thornton F C, Schaedle M and Raynal D J 1987 Effects of aluminium on red spruce seedlings in solution culture. Environ. Exp. Bot. 27(4), 489–498.
Tinker P B and Gildon A 1983 Mycorrhizal fungi and ion uptake. In Metals and Micronutrients. Uptake and Utilization by Plants. Eds. D ARobb and W SPierpoint. pp 21–32. Academic Press, London.
Turner R G 1969 Heavy metal tolerance in plants. In Ecological Aspects of the Mineral Nutrition of Plants. Ed. I HRorison. pp 399–410. Blackwell Scientific Publications, Oxford.
Wellings N P, Wearing A H and Thompson J P 1991 Vesicular-arbuscular mycorrhizae (VAM) improve phosphorus and zinc nutrition and growth of pigeonpea in a vertisol. Aust. J. Agric. Res. 42, 835–845.
Zhu H, Higginbotham K O, Dancik B P and Navratil S 1988 Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus. Can. J. Bot. 66, 588–594.
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Bücking, H., Heyser, W. The effect of ectomycorrhizal fungi on Zn uptake and distribution in seedlings of Pinus sylvestris L. Plant Soil 167, 203–212 (1994). https://doi.org/10.1007/BF00007946
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DOI: https://doi.org/10.1007/BF00007946