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Neuropeptide Y (NPY) modulatesin vitro gonadotropin in release from rainbow trout pituitary glands

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Abstract

This work investigated the action of neuropeptide Y (NPY) on thein vitro pituitary release of the maturing gonadotropic hormone (GtH) of the rainbow trout using a perifusion system employing trout balanced salt solution (pH 7.5) at 15°C and a 12.5 ml/h flow rate. In vitellogenic females a 20 minutes NPY application (10−7 M) induced a 20–30% decrease in GtH secretion. Removal of NPY was followed by a rebound in GTH secretion. On the contrary, in ovulated females, NPY (15 minutes, 10−7 M) directly stimulated GTH secretion. The greatest stimulation was obtained the day of ovulation where the stimulatory effect of NPY was similar to those induced by s.GnRH in the same conditions, reaching 400% of the basal GTH level. In vitellogenic females treated with 1-4-6 androstadien 3–7 dione, an inhibitor of aromatase activity, the pituitary response to NPY was similar to that obtained in ovulated females. Thus thein vitro action of NPY might depend on thein vivo steroidogenic environment.

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Authors and Affiliations

  1. Laboratoire de Physiologie des Poissons, INRA, Campus de Beaulieu, 35042, Rennes Cedex, France

    Bernard Breton, Tomasz Mikolajczyk & Francis Gonnet

  2. Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Unité Affiliée à l'INSERM, Université de Rouen, 76134, Mont-Saint-Aignan, France

    Jean-Michel Danger & Hubert Vaudry

  3. Institut National de la Recherche Scientifique, Departement Santé, Université du Québec, Pointe Claire, P.Q., H9R 1G6, Canada

    Serge Saint-Pierre

Authors
  1. Bernard Breton
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  2. Tomasz Mikolajczyk
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  3. Jean-Michel Danger
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  4. Francis Gonnet
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  5. Serge Saint-Pierre
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  6. Hubert Vaudry
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Breton, B., Mikolajczyk, T., Danger, JM. et al. Neuropeptide Y (NPY) modulatesin vitro gonadotropin in release from rainbow trout pituitary glands. Fish Physiol Biochem 7, 77–83 (1989). https://doi.org/10.1007/BF00004692

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