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Dynamic characteristics of serotonin and dopamine metabolism in the rainbow trout brain: a regional study using liquid chromatography with electrochemical detection

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Abstract

Aminergic metabolism was studied in discrete brain regions of the postovulated female rainbow trout using a liquid chromatography electrochemical detection method. 3 Methoxytyramine (3MT) was the major dopaminergic catabolite, suggesting that catechol-o-methyl transferase is the main dopamine (DA) catabolic enzyme. Two populations of brain regions were found: one with a high DA content and low 3MT/DA ratio (hypothalamus and telencephalon), suggesting that these regions could present a high density of DA perikarya; the other with a high 3MT/DA ratio (pituitary, preoptic area, myelencephalon and optic tectum) suggesting that these regions could present a high density of DA axonal endings. 5 Hydroxytryptamine (5HT) content differed, but an homogeneous distribution of monoamine oxidase was found in different brain regions. High 5HT content was found in the hypothalamus and telencephalon; 5HT was however not detectable in the pituitary.

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Authors and Affiliations

  1. Laboratoire de Physiologie des Régulations, UA CNRS 256Campus de Beaulieu, 35042, Rennes Cédex, France

    C. Saligaut, T. Bailhache, G. Salbert & P. Jego

  2. Laboratoire de Physiologie des Poissons (INRA), Campus de Beaulieu, 35042, Rennes Cédex, France

    B. Breton

Authors
  1. C. Saligaut
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  2. T. Bailhache
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  3. G. Salbert
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  4. B. Breton
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  5. P. Jego
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Saligaut, C., Bailhache, T., Salbert, G. et al. Dynamic characteristics of serotonin and dopamine metabolism in the rainbow trout brain: a regional study using liquid chromatography with electrochemical detection. Fish Physiol Biochem 8, 199–205 (1990). https://doi.org/10.1007/BF00004458

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