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Electromechanical effects of acetylcholine on the atrial tissues of the cultured tilapia (Oreochromis nilotica × O. aureus)

Fish Physiology and Biochemistry volume 14, pages 449–457 (1995)Cite this article

Abstract

The effects of acetylcholine (ACh) on the action potential and twitch force of atrial tissues isolated from 15 tilapia (Oreochromis nilotica × O. aureus) were studied by means of conventional microelectrode techniques. In isolated whole atrium or sinoatrial tissue, scattered pacemaker-like cells with spontaneous diastolic depolarization were found mainly near the sinoatrial junction but also occasionally throughout the atrial wall. However, most of the atrial cells recorded were myocardial fibers as judged by a stable diastolic potential and a markedly reduced action potential duration (APD) in response to low concentrations of ACh (0.1–1 μM). The shortening in APD in atrial myocardial fibers was correlated with a significant fall in twitch force in the atrial preparations. ACh at high concentrations (10–300 μM) decreased moderately the APD and the slope of diastolic depolarization of the pacemakers and prolonged the spontaneous cycle length but did not induce hyperpolarization. The negative chronotropic action of ACh was competitively inhibited by atropine, a muscarinic antagonist. The means (± SEM) negative logarithm of the dissociation constant (pKb or pA2 value) for atropine against the ACh action on muscarinic receptors were 9.10 (± 0.13) (n = 6), similar to those values obtained in mammalian atria. The present findings indicate that while the negative inotropic effects of ACh in tilapia atria are comparable to those observed in mammalian hearts, unique electrophysiological responses to ACh exist in different types of tilapia atrial cells.

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Affiliations

  1. Department of Aquaculture, College of Fisheries Science, National Taiwan Ocean University, Keelung, Taiwan

    Tsai-Chu Lin & Jia-Cheng Hsieh

  2. Department of Biomedical Engineering and Institute of Pharmacology, National Defense Medical Center, Taipei, Taiwan, R.O.C.

    Cheng-I Lin

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  1. Tsai-Chu Lin
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  2. Jia-Cheng Hsieh
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Lin, TC., Hsieh, JC. & Lin, CI. Electromechanical effects of acetylcholine on the atrial tissues of the cultured tilapia (Oreochromis nilotica × O. aureus). Fish Physiol Biochem 14, 449–457 (1995). https://doi.org/10.1007/BF00004345

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  • DOI: https://doi.org/10.1007/BF00004345

Keywords

  • fish
  • tilapia
  • heart
  • action potential
  • twitch force
  • acetylcholine
  • pA2 (atropine)
  • muscarinic receptor