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Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females

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Abstract

High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.

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References cited

  • Arai, R. 1967. Androgenic effects of 11-ketotestosterone on some sexual characteristics in the teleost, Oryzias latipes. Annot. Zool. Jap. 40: 1–5.

    Google Scholar 

  • Blaquier, J., Forchielli, E. and Dorfman, R.I. 1967. In vitro metabolism of androgens in whole human blood. Acta Endocrinol. 55: 697–704.

    Google Scholar 

  • Cochran, R.C., Zabludoff, S.D., Paynter, K.T., DiMichele, L. and Plamer, R.E. 1988. Serum hormone levels associated with spawning activity in the mummichog, Fundulus heteroclitus. Gen. Comp. Endocrinol. 70: 345–354.

    Google Scholar 

  • Colombo, L. and Colombo-Belvedere, P. 1976. Steroid biosynthesis by the ovary of the European eel, at the silver stage. Gen. Comp. Endocrinol. 28: 371–385.

    Google Scholar 

  • Cuisset, B., Pelissero, C., Nunez Rodriguez, J. and Le Menn, F. 1991. Use of plasma 11-Ketotestosterone for sex determination in Siberian sturgeon, Acipenser baeri B. In Proc. 4th Int. Symp. Reprod. Physiol. Fish. p. 272. Edited by A.P. Scott, J.P. Sumpter, D.E. Kime and M.S. Rolfe. FishSymp 91, Scheffield.

  • Cuisset, B., Pradelles, P., Kime, D.E., Kühn, E.R., Babin, P., Davail, S. and Le Menn, F. 1994. Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as label: application to the measurements of 11-ketotestosterone in the plasma of Siberian sturgeon. Comp. Biochem. Physiol. 108C: 229–241.

    Google Scholar 

  • Eckstein, B. and Eylath, U. 1970. The occurrence and biosynthesis in vitro of 11-ketotestosterone in ovarian tissue of the mullet, Mugil capito, derived from two biotopes. Gen. Comp. Endocrinol. 14: 396–403.

    Google Scholar 

  • Fostier, A., Billard, R. and Breton, B. 1984. Plasma 11-oxotestosterone and gonadotrophin in relation to the arrest of spermiation in rainbow trout (Salmo gairdneri). Gen. Comp. Endocrinol. 54: 378–381.

    Google Scholar 

  • Fostier, A., Billard, R., Breton, B., Legendre, M. and Marlot, S. 1982. Plasma 11-oxotestosterone and gonadotrophin during the beginning of spermiation in rainbow trout (Salmo gairdneri). Gen. Comp. Endocrinol. 46 428–434.

    Google Scholar 

  • Fostier, A., Jalabert, B., Billard, R., Breton, B. and Zohar, Y. 1983. The gonadal steroids. In Fish Physiology. Vol. IX, part A, pp. 277–372. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, New York.

    Google Scholar 

  • Guiguen, Y., Jalabert, B., Thouard, E. and Fostier, A. 1993. Changes in plasma gonadal steroid hormones in relation to the reproductive cycle and the sex inversion process in the protandrous seabass, Lates calcarifer. Gen. Comp. Endocrinol. 92: 327–338.

    Google Scholar 

  • Idler, D.R., Bitners, I.I. and Schmidt, P.J. 1961. 11-Ketotestosterone: an androgen for sockeye salmon. Can. J. Biochem. Physiol. 39: 1737–1742.

    Google Scholar 

  • Idler, D.R. and Sangalang, G.B. 1970. Steroids of a chondrostean: in vitro steroidogenesis in yellow bodies isolated from kidneys and along the posterior cardinal veins of the american atlantic sturgeon, Acipenser oxyrhynchus Mitchill. J. Endocrinol. 48: 627–637.

    Google Scholar 

  • Idler, D.R., Schmidt, P.J. and Ronald, A.P. 1960. Isolation and identification of 11-ketotestosterone in salmon plasma. Can. J. Biochem. Physiol. 38: 1053–1057.

    Google Scholar 

  • Kime, D.E. 1978. The hepatic catabolism of cortisol in teleost fish — Adrenal origin of 11-oxotestosterone precursors. Gen. Comp. Endocrinol. 35: 322–328.

    Google Scholar 

  • Kime, D.E. and Manning, N.J. 1982. Seasonal patterns of free and conjugated androgens in the brown trout Salmo trutta. Gen. Comp. Endocrinol. 48: 222–231.

    Google Scholar 

  • Lutes, P.B., Doroshov, S.I., Chapman, F., Harrah, J., Fitzgerald, R. and Fitzpatrick, M. 1987. Morpho-physiological predictors of ovulatory success in white sturgeon, Acipenser transmontanus Richardson. Aquaculture 66: 43–53.

    Google Scholar 

  • Mayer, I., Borg, B. and Schulz, R. 1990a. Converion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species. Gen. Comp. Endocrinol. 77: 70–74.

    Google Scholar 

  • Mayer, I., Berglund, I., Rydevik, M., Borg, B. and Schulz, R. 1990b. Plasma levels of five androgens and 17α-hydroxy,20β-dihydroprogesterone in immature and mature male baltic salmon (Salmo salar) parr, and the effects of castration/androgen-replacement in mature parr. Can. J. Zool. 86: 263–267.

    Google Scholar 

  • Mayer, I., Borg, B. and Schulz, R. 1990c. Seasonal changes in and effects of castration/androgen replacement on the plasma levels of five androgens in the male three-spined stickleback, Gasterosteus aculeatus L. Gen. Comp. Endocrinol. 79: 23–30.

    Google Scholar 

  • Mayer, I., Schmidz, M., Borg, B. and Schulz, R. 1992. Seasonal endocrine changes in male and female Arctic char (Salvelinus alpinus). I. Plasma levels of three androgens, 17α-hydroxy-20β-dihydroxyprogesterone, and 17β-estradiol. Can. J. Zool. 70: 37–42.

    Google Scholar 

  • Milewich, L. and Whisenant, M.G. 1982. Metabolism of androstenedione by human platelets: a source of potent androgens. J. Clin. Endocrinol. Metab. 54: 969–974.

    Google Scholar 

  • Pelissero, C. 1988a. Mise en place des bases méthodologiques pour l'étude de la reproduction de l'esturgeon sibérien Acipenser baeri femelle. Thèse dr. ee cycle, n° 2229, Univ. Bordeaux I.

  • Pelissero, C. and Le Menn, F. 1988b. Détermination des taux plasmatiques de stéroïdes sexuels et de la vitellogénine chez l'esturgeon sibérien Acipenser baeri élevé en pisciculture. C.R. Acad. Sci. Paris 307, serie III: 749–754.

    Google Scholar 

  • Pelissero, C., Le Menn, F. and Burzawa-Gérard, E. 1988c. Vitellogenesis and steroid levels in the Siberian sturgeon Acipenser baeri bred in fishfarm. 14th Conf. Europ. Comp. Endocrinol. Salzburg.

  • Pelissero, C., Cuisset, B. and Le Menn, F. 1989. The influence of sex steroids in commercial fish meals and fish diets on plasma concentration of estrogens and vitellogenin in cultured Siberian sturgeon Acipenser baeri. Aquat. Liv. Resourc. 2: 161–168.

    Google Scholar 

  • Quérat, B., Leloup-Hatey, J. and Hardy, A. 1983. Ovarian steroid metabolism in the immature European eel (Anguilla anguilla). Abst. Int. Symp. Reprod. Physiol. Fish, Wageningen, The Netherlands.

  • Sangalang, G.B., Weisbart, M. and Idler, D.R. 1971. Steroids of a Chondrostean: Corticosteroids and testosterone in the plasma of the american atlantic sturgeon, Acipenser oxyrhynchus Mitchill. J. Endocrinol. 51: 413–421.

    Google Scholar 

  • Schulz, R. 1986. In vitro metabolism of steroid hormones in the liver and in blood cells of male rainbow trout (Salmo gairdneri Richardson). Gen. Comp. Endocrinol. 64: 312–319.

    Google Scholar 

  • Schulz, R. and Blüm, V. 1991. Extragonadal 17β-hydroxysteroid dehydrogenase activity in rainbow trout. Gen. Comp. Endocrinol. 82: 197–205.

    Google Scholar 

  • Scott, A.P., Bye, V.J., Baynes, S.M. and Springate, J.C.R. 1980. Seasonal variations in plasma concentrations of 11-ketotestosterone and testosterone in male rainbow trout Salmo gairdneri Richardson. J. Fish Biol. 17: 495–505.

    Google Scholar 

  • Scott, A.P. and Sumpter, J.P. 1989. Seasonal variations in testicular germ cell stages and in plasma concentrations of sex steroids in male rainbow trout (Salmo gairdneri) maturing at 2 years old. Gen. Comp. Endocrinol. 73: 46–58.

    Google Scholar 

  • Sower, S.A. and Iwamoto, R.N. 1985. The identification of the sex steroid, testosterone in various commercial Salmon diets. Aquaculture 49: 11–17.

    Google Scholar 

  • Williot, P., Brun, R., Rouault, T. and Rooryck, O. 1991. Management of female spawners of the Siberian sturgeon, Acipenser baeri Brandt: first results. In Acipenser. pp. 365–379. Edited by P. Williot. CEMAGREF Publ.

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Authors and Affiliations

  1. Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France

    B. Cuisset, C. Bennetau-Pelissero & F. Le Menn

  2. Institut National de la Recherche Agronomique, Laboratoire de Physiologie des Poissons, Campus de Beaulieu, 35042, Rennes Cedex, France

    A. Fostier

  3. Division Aquaculture et Pechê, Centre National du Machinisme Agricole, du Génie Rural, des Eaux des Forêts, 50 Avenue de Verdun, BP 3, 33611, Gazinet Cedex, France

    P. Williot

  4. ENITA Bordeaux, 33170, Gradigham, France

    C. Bennetau-Pelissero

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  1. B. Cuisset
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  2. A. Fostier
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  4. C. Bennetau-Pelissero
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  5. F. Le Menn
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Cuisset, B., Fostier, A., Williot, P. et al. Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females. Fish Physiol Biochem 14, 313–322 (1995). https://doi.org/10.1007/BF00004069

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