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The effects of gonadal development and sex steroids on growth hormone secretion in the male tilapia hybrid (Oreochromis niloticus × O. aureus)

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Abstract

Profiles of plasma growth hormone (GH) in male tilapia hybrid (Oreochromis niloticus x O. aureus) were measured and compared at different times of the year. The profiles did not appear to be repetitive, however, differences in their nature were observed at the different seasons; the most erratic profiles were seen in the height of the reproductive season (July), while the peaks were more subdued in the spring and disappeared in the autumn. Peaks in male fish were more prominent than in the females when measured in July. Perifused pituitary fragments from fish with a high GSI responded to salmon gonadotropin-releasing hormone (sGnRH) analog (10 nM-1 μM), while those from fish with a low GSI barely responded to even the highest dose. Exposure of perifused pituitary fragments from sexually-regressed fish to carp growth hormone-releasing hormone (cGHRH; 0.1 μM) or sGnRH (I μM) stimulated GH release only after injection of the fish with methyl testosterone (MT; 3 injections of 0.4 mg kg 1). The same MT pretreatment did not alter the response to dopamine (DA; 1 or 10 μM). GH pituitary content in MT-treated fish was lower than in control fish, which may be explained by the higher circulating GH levels in these fish, but does not account for the increased response to the releasing hormones. Castration abolished the response of cultured pituitary cells to sGnRH (I fM-100 nM) without altering either their basal rate of secretion or circulating GH levels. Addition of steroids to the culture medium (MT or estradiol at 10 nM for 2 days) enabled a GH response to sGnRH stimulation in cells from sexually regressed fish. Pituitary cells which had not been exposed to steroids failed to respond to sGnRH, although their response to forskolin or TPA was similar to that of steroid-exposed cells. It would appear, therefore, that at least one of the effects of the sex steroids on the response to GnRH is exerted proximally to the formation of cAMP, or PKC, presumably at the level of the receptor. An increase in the number of receptors to the GH-releasing hormones, following steroid exposure, would explain also the changing nature of the GH secretory profile in different stages of the reproductive season.

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Authors and Affiliations

  1. Department of Zoology, Tel-Aviv University, Tel Aviv, 69978, Israel

    Philippa Melamed, Noa Eliahu, Michal Ofir, Berta Levavi-Sivan & Zvi Yaron

  2. Eurogentic, S.A., B-4102, Seraing, Belgium

    Jean Smal

  3. Laboratoire de Biologie Moleculaire et de Genie Génétique, Université de Liège, B-4000, Belgium

    Francoise Rentier-Delrue

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  1. Philippa Melamed
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  2. Noa Eliahu
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  3. Michal Ofir
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  4. Berta Levavi-Sivan
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  6. Francoise Rentier-Delrue
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  7. Zvi Yaron
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Correspondence to Philippa Melamed.

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Melamed, P., Eliahu, N., Ofir, M. et al. The effects of gonadal development and sex steroids on growth hormone secretion in the male tilapia hybrid (Oreochromis niloticus × O. aureus). Fish Physiol Biochem 14, 267–277 (1995). https://doi.org/10.1007/BF00004065

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