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A strategy for high cell density culture of heterotrophic microalgae with inhibitory substrates

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Abstract

Substrate inhibition is one of the major problems preventing high cell densities of microalgae in heterotrophic culture, so the possibility of overcoming the problem by various culture techniques was examined. It was found that perfusion culture may be the most appropriate technique for high cell densities in heterotrophic culture using inhibitory substrates. An experimental example in which a hollow fibre cell recycle system (HFCRS) was employed to achieve high cell densities of Chlamydomonas reinhardtii on acetate under heterotrophic conditions of growth was demonstrated. The cell density in the HFCRS was much higher than that reported in the literature for this species.

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Authors and Affiliations

  1. Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong

    Feng Chen

  2. Department of Chemical Engineering, The University of Queensland, Brisbane, QLD 4072, Australia

    Michael R. Johns

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  1. Feng Chen
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  2. Michael R. Johns
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Chen, F., Johns, M.R. A strategy for high cell density culture of heterotrophic microalgae with inhibitory substrates. J Appl Phycol 7, 43–46 (1995). https://doi.org/10.1007/BF00003548

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  • DOI: https://doi.org/10.1007/BF00003548

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