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Relationship between vitamin C and plasma concentrations of testosterone in female rainbow trout, Oncorhynchus mykiss

Fish Physiology and Biochemistry volume 14, pages 409–414 (1995)Cite this article

Abstract

Two-year old rainbow trout females were fed diets containing 0, 30, 110, 220, 440 and 870 mg kg-1 ascorbyl-2-monophosphate Mg+ salt (groups 1, 2, 3, 4, 5, and 6, respectively) from August until March. At the time of spawning (February–March) blood was sampled and the ovulating females were hand stripped. Estradiol (E2) and testosterone (T) concentrations in plasma, and ascorbic acid (AA) concentrations in plasma and eggs were determined. The mean plasma concentrations of T were higher in group 4, 5, or 6 than in group 2 or 3 (p < 0.05). Moreover, the average plasma concentration of T in fish fed the diets with AA level below National Research Council (NRC) recommendations (groups 1, 2 and 3) was significantly lower (p<0.01) than the average plasma concentration in fish fed diets with AA level above NRC recommendations (groups 4, 5, and 6). These data are consistent with the hypothesis that AA can influence production of steroids in female rainbow trout.

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References cited

  • Aten, R.F., Duarte, K.M. and Behrman, H.R. 1992. Regulation of ovarian antioxidant vitamins, reduced glutathione, and lipid peroxidation by luteinizing hormone and prostaglandin F2α. Biol. Reprod. 46: 401–407.

    Google Scholar 

  • Blom, J.H. and Dabrowski, K. 1995. Reproductive success of female rainbow trout (Oncorhynchus mykiss) in response to graded dietary ascorbyl mono-phosphate levels. Biol. Reprod. 52: 1073–1080.

    Google Scholar 

  • Bromage, N. and Cumaranatunga, R. 1988. Egg production in the rainbow trout. In Recent Advances in Aquaculture. pp. 63–138. Edited by F. Muir and J. Roberts, Croom Helm, London.

    Google Scholar 

  • Bushway, R.J., King, J.M., Perkins, B. and Krishnan, M. 1988. High-performance liquid chromatographic determination of ascorbic acid in fruits, vegetables and juices. J. Liquid Chrom. 11: 3415–3423.

    Google Scholar 

  • Butcher, R., Collins, W.E. and Fugo, N.W. 1974. Plasma concentration of LH, FSH, prolactin, progesterone and estradiol-17β throughout the 4-day estrous cycle of the rat. Endocrinology 94: 1704–1708.

    Google Scholar 

  • Carlson, J.C., Wu, X.M. and Sawada, M. 1993. Oxygen radicals and the control of ovarian corpus luteum function. Free Rad. Biol. Med. 14: 79–84.

    Google Scholar 

  • Dabrowski, K. 1991. Ascorbic acid status in high-mountain charr, Salvelinus alpinus, in relation to the reproductive cycle. Env. Biol. Fish. 31: 213–217.

    Google Scholar 

  • Dabrowski, K. and Hinterleitner, S. 1989. Applications of a simultaneous assay of ascorbic acid, dehydroascorbic acid and ascorbic sulfate in biological materials. Analyst 114: 83–87.

    Google Scholar 

  • Dabrowski, K. and Blom, J.H. 1994. Ascorbic acid deposition in rainbow trout (Oncorhynchus mykiss) eggs and survival of embryos. Comp. Biochem. Physiol. 108A: 129–135.

    Google Scholar 

  • Dabrowski, K., Matusiewicz, M. and Blom, J.H. 1994. Hydrolysis, absorption and bioavailability of ascorbic acid esters in fish. Aquaculture 124: 169–192.

    Google Scholar 

  • Eskelinen, P. 1989. Effects of different diets on egg production and egg quality of Atlantic salmon (Salmo salar L.). Aquaculture 79: 275–281.

    Google Scholar 

  • Fitzpatrick, M.S., Redding, J.M., Ratti, F.D. and Schreck, C.B. 1987. Plasma testosterone concentration predicts the ovulatory response to gonadotropin-releasing hormone analog. Can. J. Fish. Aquat. Sci. 44: 1351–1357.

    Google Scholar 

  • Fostier, A., Jalabert, B., Billard, R., Breton, B. and Zohar, Y. 1983. The gonadal steroids. In Fish Physiology. pp. 227–347. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press.

  • Halliwell, B. and Gutteridge, J.M.C. 1989. Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity. In Free Radicals in Biology and Medicine. pp. 86–187. Clarendon Press, Oxford.

    Google Scholar 

  • Hornsby, P.J., Harris, S.E. and Aldern, K.A. 1985. The role of ascorbic acid in the function of the adrenal cortex: studies in adrenocortical cells in culture. Endocrinology 117: 1264–1271.

    Google Scholar 

  • Kanazawa, Y., Kitawa, M., Mori, T., Inukai, Y., Imaoka, S., Funae, Y. and Kamataki, T. 1991. Ascorbic acid deficiency decreases specific forms of cytochrome P-450 in liver microsomes of guinea pigs. Molec. Pharmacol. 39: 456–460.

    Google Scholar 

  • Komarov, I.P. and Knyazeva, L.M. 1984. Effect of vitamin C-enriched feed on the physiological state of spawning rainbow trout. Hydrobiol. J. 2: 43–48.

    Google Scholar 

  • National Research Council. 1993. Nutrient Requirements of Fish. National Academy Press, Washington.

    Google Scholar 

  • Ottobre, J.S., Houmard, B.S. and Ottobre, A.C. 1989. Luteal production of steroids and prostaglandins during simulated early pregnancy in the primate: differential regulation of steroid production by chorionic gonadotropin. Biol. Reprod. 41: 393–400.

    Google Scholar 

  • Sandnes, K., Ulgenes, Y., Braekkan, O.R. and Utne, F. 1984. The effect of ascorbic acid supplementation in broodstock feed on reproduction of rainbow trout (Salmo gairdneri). Aquaculture 43: 167–177.

    Google Scholar 

  • Sandnes, K. 1991. Vitamin C in fish nutrition — a review. Fisk. Dir. Skr., Ser. Ernaering 4: 3–32.

    Google Scholar 

  • Scott, A.P., Bye, V.J. and Baynes, S.M. 1980. Seasonal variations in sex steroids of female rainbow trout (Salmo gairdneri Richardson). J. Fish Biol. 17: 587–592.

    Google Scholar 

  • Sheldrick, E.L. and Flint, A.P.F. 1989. Post-translational processing of oxytocin-neurophysin prohormone in the ovine corpus luteum: activity of peptidyl glycine α-amidating monooxygenase and concentrations of its cofactor, ascorbic acid. J. Endocrinol. 122: 313–322.

    Google Scholar 

  • Soliman, A.K., Jauncey, K. and Roberts, R.J. 1986. The effects of dietary ascorbic acid supplementation on hatchability, survival rate and fry performance in Oreochromis mossambicus (Peters). Aquaculture 59: 197–208.

    Google Scholar 

  • Trudeau, V.L., Murthy, C.H., Habibi, H.R., Sloley, B.D. and Peter, R.E. 1993. Effects of sex steroid treatments on gonadotropin-releasing hormone-stimulated gonadotropin secretion from the goldfish pituitary. Biol. Reprod. 48: 300–307.

    Google Scholar 

  • Waagbo, R., Thorsen, T. and Sandnes, K. 1989. Role of dietary ascorbic acid in vitellogenesis in rainbow trout (Salmo gairdneri). Aquaculture 80: 301–314.

    Google Scholar 

  • Young, G., Kagawa, H. and Nagahama, Y. 1982. Oocyte maturation in the amago salmon (Oncorhynchus rhodurus): in vitro effects of salmon gonadotropin, steroids, and cyanoketone (an inhibitor of 3β-hydroxy-delta5-steroid dehydrogenase). J. Exp. Zool. 224: 265–275.

    Google Scholar 

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Authors and Affiliations

  1. School of Natural Resources, The Ohio State University, Columbus, Ohio, 43210, USA

    K. Dabrowski, R. E. Ciereszko & J. H. Blom

  2. Department of Animal Sciences, The Ohio State University, Columbus, Ohio, 43210, USA

    J. S. Ottobre

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  1. K. Dabrowski
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  2. R. E. Ciereszko
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  3. J. H. Blom
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  4. J. S. Ottobre
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Dabrowski, K., Ciereszko, R.E., Blom, J.H. et al. Relationship between vitamin C and plasma concentrations of testosterone in female rainbow trout, Oncorhynchus mykiss . Fish Physiol Biochem 14, 409–414 (1995). https://doi.org/10.1007/BF00003378

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  • DOI: https://doi.org/10.1007/BF00003378

Keywords

  • ascorbic acid
  • testosterone
  • spawning
  • salmonid